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Role of GalNAc4S-6ST in Astrocytic Tumor Progression
http://hdl.handle.net/10091/00019824
http://hdl.handle.net/10091/00019824b93f0271-7a8e-4e5a-998c-564f78db4938
名前 / ファイル | ライセンス | アクション |
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Role_of_GalNAc4S-6ST_in_Astrocytic_Tumor_Progression.pdf (831.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-09-20 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Role of GalNAc4S-6ST in Astrocytic Tumor Progression | |||||
言語 | ||||||
言語 | eng | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1371/journal.pone.0054278 | |||||
関連名称 | 10.1371/journal.pone.0054278 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Kobayashi, Tatsuya
× Kobayashi, Tatsuya× Yan, Huimin× Kurahashi, Yasuhiro× Ito, Yuki× Maeda, Hiroshi× Tada, Tsuyoshi× Hongo, Kazuhiro× Nakayama, Jun |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Tada, Tsuyoshi | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OeLFupkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Hongo, Kazuhiro | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jecFHCkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Nakayama, Jun | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HCApHFkh.html | |||||
出版者 | ||||||
出版者 | PUBLIC LIBRARY SCIENCE | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PLOS ONE. 8(1):e54278 (2013) | |||||
書誌情報 |
PLOS ONE 巻 8, 号 1, p. e54278, 発行日 2013-01-16 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | N-Acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST) is the sulfotransferase responsible for biosynthesis of highly sulfated chondroitin sulfate CS-E. Although involvements of CS-E in neuronal cell functions have been extensively analyzed, the role of GalNAc4S-6ST in astrocytic tumor progression remains unknown. Here, we reveal that GalNAc4S-6ST transcripts were detected in astrocytic tumors derived from all 30 patients examined using quantitative reverse transcription-PCR analysis. Patients with high GalNAc4S-6ST mRNA expression had significantly worse outcome compared with patients with low expression, and multivariate survival analysis disclosed that GalNAc4S-6ST is an independent poor prognostic factor for astrocytic tumors. We then tested whether CS-E enhanced haptotaxic migration of glioblastoma U251-MG cells that endogenously express both the CS-E's scaffold tyrosine phosphatase xi (PTP xi) and GalNAc4S-6ST, in the presence of CS-E's preferred ligands, pleiotrophin (PTN) or midkine (MK), using a modified Boyden chamber method. Haptotaxic stimulation of cell migration by PTN was most robust on control siRNA-transfected U251-MG cells, while that enhancing effect was cancelled following transduction of GalNAc4S-6ST siRNA. Similar results were obtained using MK, suggesting that both PTN and MK enhance migration of U251-MG cells by binding to CS-E. We also found that PTP xi as well as PTN and MK were frequently expressed in astrocytic tumor cells. Thus, our findings indicate that GalNAc4S-6ST mRNA expressed by astrocytic tumor cells is associated with poor patient prognosis likely by enhancing CS-E-mediated tumor cell motility in the presence of PTN and/or MK. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 1932-6203 | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://pubmed.ncbi.nlm.nih.gov/23349846 | |||||
関連名称 | 23349846 | |||||
権利 | ||||||
権利情報 | © 2013 Kobayashi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000313738900059 |