Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2018-01-10 |
タイトル |
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タイトル |
Multiple aster formation is frequently observed in bovine oocytes retrieved from 1 day-stored ovaries |
言語 |
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言語 |
eng |
DOI |
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関連識別子 |
https://doi.org/10.1017/S096719941400080X |
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関連名称 |
10.1017/S096719941400080X |
キーワード |
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主題 |
Blastocysts, Bovine IVF, Multiple asters, Ovary storage, ROCK inhibitor (Y-27632) |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Hara, H
Tagiri, M
Hirabayashi, M
Hochi, S
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信州大学研究者総覧へのリンク |
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氏名 |
Hochi, S |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html |
出版者 |
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出版者 |
CAMBRIDGE UNIV PRESS |
引用 |
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内容記述 |
ZYGOTE.24(1):115-120(2015) |
書誌情報 |
ZYGOTE
巻 24,
号 1,
p. 115-120,
発行日 2015-03-03
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抄録 |
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内容記述 |
We have recently reported that multiple aster formation after IVF was one of the factors negatively affecting to developmental competence of vitrified-warmed bovine matured oocytes, and that a short-term culture of the post-warm oocytes with an inhibitor of Rho-associated coiled-coil kinase (ROCK) suppressed the multiple aster formation and improved the blastocyst yield. The present study was conducted to investigate whether increased multiple aster formation following IVF can be involved in impaired developmental competence of stored ovary-derived bovine oocytes. Oocytes retrieved from 1-day stored ovaries had lower developmental potential to Day 8 blastocysts when compared with those from fresh ovaries (37 vs 63%). Immunostaining against -tubulin 10 h post-IVF revealed that the higher incidence of multiple aster formation occurred in oocytes retrieved from stored ovaries than fresh ovaries (31 vs 15%). Treatment of post-IVM oocytes with ROCK inhibitor for 2 h significantly suppressed the incidence of multiple aster formation (10 vs 32% in control group). However, the suppressing effect of ROCK inhibitor on multiple aster formation in IVM/IVF oocytes did not improve blastocyst yield from stored ovary-derived oocytes (41 vs 37% in control group). These results suggest that higher incidence of multiple aster formation by bovine ovary storage was not responsible for decreased developmental competence of IVF oocytes. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0967-1994 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA10958262 |
PubMed |
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関連識別子 |
https://www.ncbi.nlm.nih.gov/pubmed/25732862 |
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関連名称 |
25732862 |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000367182000012 |