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  1. 080 繊維学部
  2. 0801 学術論文

Multiple aster formation is frequently observed in bovine oocytes retrieved from 1 day-stored ovaries

http://hdl.handle.net/10091/00020125
http://hdl.handle.net/10091/00020125
a79bb837-e0d2-48a9-ab56-012aaa6e28e2
名前 / ファイル ライセンス アクション
24580407_02.pdf 24580407_02.pdf (455.7 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2018-01-10
タイトル
タイトル Multiple aster formation is frequently observed in bovine oocytes retrieved from 1 day-stored ovaries
言語
言語 eng
DOI
関連識別子 https://doi.org/10.1017/S096719941400080X
関連名称 10.1017/S096719941400080X
キーワード
主題 Blastocysts, Bovine IVF, Multiple asters, Ovary storage, ROCK inhibitor (Y-27632)
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Hara, H

× Hara, H

Hara, H

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Tagiri, M

× Tagiri, M

Tagiri, M

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Hirabayashi, M

× Hirabayashi, M

Hirabayashi, M

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Hochi, S

× Hochi, S

Hochi, S

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信州大学研究者総覧へのリンク
氏名 Hochi, S
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html
出版者
出版者 CAMBRIDGE UNIV PRESS
引用
内容記述 ZYGOTE.24(1):115-120(2015)
書誌情報 ZYGOTE

巻 24, 号 1, p. 115-120, 発行日 2015-03-03
抄録
内容記述 We have recently reported that multiple aster formation after IVF was one of the factors negatively affecting to developmental competence of vitrified-warmed bovine matured oocytes, and that a short-term culture of the post-warm oocytes with an inhibitor of Rho-associated coiled-coil kinase (ROCK) suppressed the multiple aster formation and improved the blastocyst yield. The present study was conducted to investigate whether increased multiple aster formation following IVF can be involved in impaired developmental competence of stored ovary-derived bovine oocytes. Oocytes retrieved from 1-day stored ovaries had lower developmental potential to Day 8 blastocysts when compared with those from fresh ovaries (37 vs 63%). Immunostaining against -tubulin 10 h post-IVF revealed that the higher incidence of multiple aster formation occurred in oocytes retrieved from stored ovaries than fresh ovaries (31 vs 15%). Treatment of post-IVM oocytes with ROCK inhibitor for 2 h significantly suppressed the incidence of multiple aster formation (10 vs 32% in control group). However, the suppressing effect of ROCK inhibitor on multiple aster formation in IVM/IVF oocytes did not improve blastocyst yield from stored ovary-derived oocytes (41 vs 37% in control group). These results suggest that higher incidence of multiple aster formation by bovine ovary storage was not responsible for decreased developmental competence of IVF oocytes.
資源タイプ(コンテンツの種類)
ISSN
収録物識別子タイプ ISSN
収録物識別子 0967-1994
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10958262
PubMed
識別子タイプ PMID
関連識別子 https://www.ncbi.nlm.nih.gov/pubmed/25732862
関連名称 25732862
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000367182000012
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