| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2018-01-19 |
| タイトル |
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タイトル |
A Baculovirus Immediate-Early Gene, ie1, Promoter Drives Efficient Expression of a Transgene in Both Drosophila melanogaster and Bombyx mori |
| 言語 |
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言語 |
eng |
| DOI |
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関連識別子 |
https://doi.org/10.1371/journal.pone.0049323 |
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関連名称 |
10.1371/journal.pone.0049323 |
| 資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
| 著者 |
Masumoto, Mika
Ohde, Takahiro
Shiomi, Kunihiro
Yaginuma, Toshinobu
Niimi, Teruyuki
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| 信州大学研究者総覧へのリンク |
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氏名 |
Shiomi, Kunihiro |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.WCnpjFkV.html |
| 出版者 |
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出版者 |
Public Library of Science |
| 引用 |
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内容記述 |
PLOS ONE.7(11):e49323(2012) |
| 書誌情報 |
PLOS ONE
巻 7,
号 11,
p. e49323,
発行日 2012-11-13
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| 抄録 |
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内容記述 |
Many promoters have been used to drive expression of heterologous transgenes in insects. One major obstacle in the study of non-model insects is the dearth of useful promoters for analysis of gene function. Here, we investigated whether the promoter of the immediate-early gene, ie1, from the Bombyx mori nucleopolyhedrovirus (BmNPV) could be used to drive efficient transgene expression in a wide variety of insects. We used a piggyBac-based vector with a 3xP3-DsRed transformation marker to generate a reporter construct; this construct was used to determine the expression patterns driven by the BmNPV ie1 promoter; we performed a detailed investigation of the promoter in transgene expression pattern in Drosophila melanogaster and in B. mori. Drosophila and Bombyx belong to different insect orders (Diptera and Lepidoptera, respectively); however, and to our surprise, ie1 promoter-driven expression was evident in several tissues (e.g., prothoracic gland, midgut, and tracheole) in both insects. Furthermore, in both species, the ie1 promoter drove expression of the reporter gene from a relatively early embryonic stage, and strong ubiquitous ie1 promoter-driven expression continued throughout the larval, pupal, and adult stages by surface observation. Therefore, we suggest that the ie1 promoter can be used as an efficient expression driver in a diverse range of insect species. |
| 資源タイプ（コンテンツの種類） |
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1932-6203 |
| PubMed |
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識別子タイプ |
PMID |
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関連識別子 |
https://www.ncbi.nlm.nih.gov/pubmed/23152896 |
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関連名称 |
23152896 |
| 権利 |
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権利情報 |
© 2012 Masumoto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000311234600035 |
24380031_04.pdf (1.3 MB)
