{"_buckets": {"deposit": "bc098cca-8402-480e-9e3d-e4c3b68853db"}, "_deposit": {"id": "19940", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "19940"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00019940"}, "item_14_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-03-20", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_14_date_granted_8": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5", "attribute_value_mlt": [{"subitem_dategranted": "2018-03-20"}]}, "item_14_degree_grantor_10": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u6a5f\u95a2", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "\u4fe1\u5dde\u5927\u5b66(Shinshu university)"}]}]}, "item_14_degree_name_9": {"attribute_name": "\u5b66\u4f4d\u540d", "attribute_value_mlt": [{"subitem_degreename": "\u535a\u58eb\uff08\u4fdd\u5065\u5b66\uff09"}]}, "item_14_description_26": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u96d1\u8a8c\u306b\u767a\u8868\u3002JOURNAL OF MEDICAL MICROBIOLOGY. 67(1):33-39 (2018); doi:10.1099/jmm.0.000634.", "subitem_description_type": "Other"}]}, "item_14_description_37": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Thesis", "subitem_description_type": "Other"}]}, "item_14_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "\u6839\u5cb8\u3000\u9054\u54c9. Characterization of clinically isolated thymidine-dependent small-colony variants of Escherichia coli producing extended-spectrum \u03b2-lactamase(ESBL)\uff08\u81e8\u5e8a\u691c\u4f53\u3088\u308a\u5206\u96e2\u3055\u308c\u305f\u57fa\u8cea\u62e1\u5f35\u578b\u03b2-\u30e9\u30af\u30bf\u30de\u30fc\u30bc\u7523\u751f\u6027Escherichia coli\u306e\u30c1\u30df\u30b8\u30f3\u8981\u6c42\u6027small-colony variants\u306e\u7279\u5fb4\uff09. \u4fe1\u5dde\u5927\u5b66, 2018, \u535a\u58eb\u8ad6\u6587.", "subitem_description_type": "Other"}]}, "item_14_dissertation_number_7": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7", "attribute_value_mlt": [{"subitem_dissertationnumber": "13601\u7532\u7b2c22\u53f7"}]}, "item_14_link_74": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000425273400004"}]}, "item_14_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u4fe1\u5dde\u5927\u5b66"}]}, "item_14_relation_54": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "29143727"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=29143727", "subitem_relation_type_select": "PMID"}}]}, "item_14_relation_55": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1099/jmm.0.000634"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1099/jmm.0.000634", "subitem_relation_type_select": "DOI"}}]}, "item_14_rights_69": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2018 The Authors. Published by the Microbiology Society."}]}, "item_14_select_71": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_14_text_11": {"attribute_name": "\u5b66\u4f4d\u306e\u533a\u5206", "attribute_value_mlt": [{"subitem_text_value": "Doctoral"}]}, "item_14_text_12": {"attribute_name": "\u5b66\u4f4d\u306e\u5206\u91ce", "attribute_value_mlt": [{"subitem_text_value": "\u4fdd\u5065\u5b66"}]}, "item_14_text_13": {"attribute_name": "\u5b66\u4f4d\u306e\u5831\u544a\u756a\u53f7", "attribute_value_mlt": [{"subitem_text_value": "\u7532\u7b2c22\u53f7"}]}, "item_14_text_76": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "small-colony variants; thymidine-dependent; ESBL; Escherichia coli"}]}, "item_14_text_77": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "STAPHYLOCOCCUS-AUREUS; THYMIDYLATE SYNTHASE; RESISTANT ENTEROBACTERIACEAE; KLEBSIELLA-PNEUMONIAE; CYSTIC-FIBROSIS; INFECTION; 5-FLUORO-2\u0027-DEOXYURIDYLATE; PHENOTYPE; BACTERIA; CTX-M-3"}]}, "item_14_textarea_75": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Purpose. Thymidine-dependent small-colony variants (TD-SCVs) are difficult to detect or test for antimicrobial susceptibility. We investigated the characteristics of clonal TD-SCVs of Escherichia coli, both with and without bla(CTX-M-3), isolated from a patient. Methodology. Mutation in the thyA gene was analysed by sequencing, and morphological abnormalities in the colonies and cells of the isolates were examined. Additionally, conjugational transfer experiments were performed to prove the horizontal transferability of plasmids harbouring resistance genes. Results. The TD-SCVs contained a single nucleotide substitution in the thyA gene, c.62G\u003eA, corresponding to p.Arg21His. Morphologically, their colonies were more translucent and flattened than those of the wild-type strain. In addition, cells of the TD-SCVs were swollen and elongated, sometimes with abnormal and incomplete divisions; a large amount of cell debris was also observed. Changing c.62G\u003eA back to the wild-type sequence reversed these abnormalities. Conjugational transfer experiments showed that the TD-SCV of E. coli with bla(CTX-M-3) failed to transfer bla(CTX-M-3) to E. coli CSH2. However, the TD-SCV of E. coli without bla(CTX-M-3) experimentally received the plasmid encoding blaSHV-18 from Klebsiella pneumoniae ATCC 700603 and transferred it to E. coli CSH2. Conclusion. Mutation in the thyA gene causes morphological abnormalities in the colonies and cells of E. coli, as well as inducing thymidine auxotrophy. In addition, TD-SCVs horizontally transmit plasmids encoding resistance genes. It is important to detect TD-SCVs based on their characteristics because they serve as reservoirs of transferable antibiotic resistance plasmids."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u6839\u5cb8, \u9054\u54c9"}], "nameIdentifiers": [{"nameIdentifier": "105893", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-06-27"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "15MK005C_yoshi.pdf", "filesize": [{"value": "144.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 144300.0, "url": {"label": "\u5185\u5bb9\u306e\u8981\u65e8", "url": "https://soar-ir.repo.nii.ac.jp/record/19940/files/15MK005C_yoshi.pdf"}, "version_id": "d3e7325f-fa39-40a3-b173-132fc34da6ae"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-06-27"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "15MK005C_shinsa.pdf", "filesize": [{"value": "160.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 160600.0, "url": {"label": "\u5be9\u67fb\u7d50\u679c\u306e\u8981\u65e8", "url": "https://soar-ir.repo.nii.ac.jp/record/19940/files/15MK005C_shinsa.pdf"}, "version_id": "7a081e38-2130-47bb-b4fc-6335af6dba0d"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-01-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "15MK005C_ronbun.pdf", "filesize": [{"value": "1.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "\u00a9 2018 The Authors. 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