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PDGF-induced migration of synthetic vascular smooth muscle cells through c-Src-activated L-type Ca2+ channels with full-length CaV1.2 C-terminus\uff08\u8840\u5c0f\u677f\u7531\u6765\u5897\u6b96\u56e0\u5b50\u306b\u3088\u308b\u5408\u6210\u578b\u8840\u7ba1\u5e73\u6ed1\u7b4b\u7d30\u80de\u306e\u904a\u8d70\u306f\u3001c-Src\u3067\u6d3b\u6027\u5316\u3055\u308c\u305f\u5168\u9577\u578bCav1.2 C\u672b\u7aef\u3092\u6709\u3059\u308bL\u578bCa2+\u30c1\u30e3\u30cd\u30eb\u3092\u4ecb\u3059\u308b\uff09. \u4fe1\u5dde\u5927\u5b66, 2017, \u535a\u58eb\u8ad6\u6587.", "subitem_description_type": "Other"}]}, "item_14_dissertation_number_7": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7", "attribute_value_mlt": [{"subitem_dissertationnumber": "13601\u7532\u7b2c1125\u53f7"}]}, "item_14_link_74": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000431873800005"}]}, "item_14_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u4fe1\u5dde\u5927\u5b66"}]}, "item_14_relation_54": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "29441404"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=29441404", "subitem_relation_type_select": "PMID"}}]}, "item_14_relation_55": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1007/s00424-018-2114-3"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1007/s00424-018-2114-3", "subitem_relation_type_select": "DOI"}}]}, "item_14_rights_69": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "The final publication is available at link.springer.com"}]}, "item_14_select_71": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_14_text_11": {"attribute_name": "\u5b66\u4f4d\u306e\u533a\u5206", "attribute_value_mlt": [{"subitem_text_value": "Doctoral"}]}, "item_14_text_12": {"attribute_name": "\u5b66\u4f4d\u306e\u5206\u91ce", "attribute_value_mlt": [{"subitem_text_value": "\u533b\u5b66"}]}, "item_14_text_13": {"attribute_name": "\u5b66\u4f4d\u306e\u5831\u544a\u756a\u53f7", "attribute_value_mlt": [{"subitem_text_value": "\u7532\u7b2c1125\u53f7"}]}, "item_14_text_76": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Vascular smooth muscle cells; Platelet-derived growth factor; Ca(V)1.2 channels; c-Src"}]}, "item_14_text_77": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "HIGH BLOOD-PRESSURE; CALCIUM-CHANNELS; SUBUNIT; KINASE; TRANSCRIPTION; CHEMOTAXIS; MECHANISMS; ARTERIES; DOMAIN"}]}, "item_14_textarea_75": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "In atherosclerosis, vascular smooth muscle cells (VSMC) migrate from the media toward the intima of the arteries in response to cytokines, such as platelet-derived growth factor (PDGF). However, molecular mechanism underlying the PDGF-induced migration of VSMCs remains unclear. The migration of rat aorta-derived synthetic VSMCs, A7r5, in response to PDGF was potently inhibited by a Ca(V)1.2 channel inhibitor, nifedipine, and a Src family tyrosine kinase (SFK)/Abl inhibitor, bosutinib, in a less-than-additive manner. PDGF significantly increased Ca(V)1.2 channel currents without altering Ca(V)1.2 protein expression levels in A7r5 cells. This reaction was inhibited by C-terminal Src kinase, a selective inhibitor of SFKs. In contractile VSMCs, the C-terminus of Ca(V)1.2 is proteolytically cleaved into proximal and distal C-termini (PCT and DCT, respectively). Clipped DCT is noncovalently reassociated with PCT to autoinhibit the channel activity. Conversely, in synthetic A7r5 cells, full-length Ca(V)1.2 (Ca(V)1.2FL) is expressed much more abundantly than truncated Ca(V)1.2. In a heterologous expression system, c-Src activated Ca(V)1.2 channels composed of Ca(V)1.2FL but not truncated Ca(V)1.2 (Ca(V)1.2 Delta 1763) or Ca(V)1.2 Delta 1763 plus clipped DCT. Further, c-Src enhanced the coupling efficiency between the voltage-sensing domain and activation gate of Ca(V)1.2FL channels by phosphorylating Tyr1709 and Tyr1758 in PCT. Compared with Ca(V)1.2 Delta 1763, c-Src could more efficiently bind to and phosphorylate Ca(V)1.2FL irrespective of the presence or absence of clipped DCT. Therefore, in atherosclerotic lesions, phenotypic switching of VSMCs may facilitate pro-migratory effects of PDGF on VSMCs by suppressing posttranslational Ca(V)1.2 modifications."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u90ed, \u6681\u5149"}], "nameIdentifiers": [{"nameIdentifier": "105923", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-06-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "13MH205F_yoshi.pdf", "filesize": [{"value": "123.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 123800.0, "url": {"label": "\u5185\u5bb9\u306e\u8981\u65e8", "url": "https://soar-ir.repo.nii.ac.jp/record/19970/files/13MH205F_yoshi.pdf"}, "version_id": "a6c1d2d3-d06a-48c3-b44d-6d118a852117"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-06-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "13MH205F_shinsa.pdf", "filesize": [{"value": "134.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 134800.0, "url": {"label": "\u5be9\u67fb\u7d50\u679c\u306e\u8981\u65e8", "url": "https://soar-ir.repo.nii.ac.jp/record/19970/files/13MH205F_shinsa.pdf"}, "version_id": "75fc24e7-530a-4287-9b2d-060eb599026f"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-02-13"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "13MH205F_ronbun.pdf", "filesize": [{"value": "1.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "The final publication is available at link.springer.com", "licensetype": "license_free", "mimetype": "application/pdf", "size": 1700000.0, "url": {"label": "\u535a\u58eb\u8ad6\u6587\u306e\u5168\u6587", "url": "https://soar-ir.repo.nii.ac.jp/record/19970/files/13MH205F_ronbun.pdf"}, "version_id": "c5f5b549-686c-4c57-b40d-400be78b744c"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "PDGF-induced migration of synthetic vascular smooth muscle cells through c-Src-activated L-type Ca2+ channels with full-length CaV1.2 C-terminus\uff08\u8840\u5c0f\u677f\u7531\u6765\u5897\u6b96\u56e0\u5b50\u306b\u3088\u308b\u5408\u6210\u578b\u8840\u7ba1\u5e73\u6ed1\u7b4b\u7d30\u80de\u306e\u904a\u8d70\u306f\u3001c-Src\u3067\u6d3b\u6027\u5316\u3055\u308c\u305f\u5168\u9577\u578bCav1.2 C\u672b\u7aef\u3092\u6709\u3059\u308bL\u578bCa2+\u30c1\u30e3\u30cd\u30eb\u3092\u4ecb\u3059\u308b\uff09", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "PDGF-induced migration of synthetic vascular smooth muscle cells through c-Src-activated L-type Ca2+ channels with full-length CaV1.2 C-terminus\uff08\u8840\u5c0f\u677f\u7531\u6765\u5897\u6b96\u56e0\u5b50\u306b\u3088\u308b\u5408\u6210\u578b\u8840\u7ba1\u5e73\u6ed1\u7b4b\u7d30\u80de\u306e\u904a\u8d70\u306f\u3001c-Src\u3067\u6d3b\u6027\u5316\u3055\u308c\u305f\u5168\u9577\u578bCav1.2 C\u672b\u7aef\u3092\u6709\u3059\u308bL\u578bCa2+\u30c1\u30e3\u30cd\u30eb\u3092\u4ecb\u3059\u308b\uff09"}]}, "item_type_id": "14", "owner": "1", "path": ["461/848/849"], "permalink_uri": "http://hdl.handle.net/10091/00020731", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2018-06-28"}, "publish_date": "2018-06-28", "publish_status": "0", "recid": "19970", "relation": {}, "relation_version_is_last": true, "title": ["PDGF-induced migration of synthetic vascular smooth muscle cells through c-Src-activated L-type Ca2+ channels with full-length CaV1.2 C-terminus\uff08\u8840\u5c0f\u677f\u7531\u6765\u5897\u6b96\u56e0\u5b50\u306b\u3088\u308b\u5408\u6210\u578b\u8840\u7ba1\u5e73\u6ed1\u7b4b\u7d30\u80de\u306e\u904a\u8d70\u306f\u3001c-Src\u3067\u6d3b\u6027\u5316\u3055\u308c\u305f\u5168\u9577\u578bCav1.2 C\u672b\u7aef\u3092\u6709\u3059\u308bL\u578bCa2+\u30c1\u30e3\u30cd\u30eb\u3092\u4ecb\u3059\u308b\uff09"], "weko_shared_id": 3}