{"_buckets": {"deposit": "5d094978-a934-4c06-81bd-f8e8b2c734fc"}, "_deposit": {"id": "19970", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "19970"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00019970", "sets": ["849"]}, "author_link": ["105923"], "item_14_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-03-31", "bibliographicIssueDateType": "Issued"}}]}, "item_14_date_granted_8": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2017-03-31"}]}, "item_14_degree_grantor_10": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "信州大学(Shinshu university)"}]}]}, "item_14_degree_name_9": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（医学）"}]}, "item_14_description_26": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "雑誌に発表。PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY. 470(6):909-921 (2018); doi:10.1007/s00424-018-2114-3.", "subitem_description_type": "Other"}]}, "item_14_description_37": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Thesis", "subitem_description_type": "Other"}]}, "item_14_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "郭　暁光. PDGF-induced migration of synthetic vascular smooth muscle cells through c-Src-activated L-type Ca2+ channels with full-length CaV1.2 C-terminus（血小板由来増殖因子による合成型血管平滑筋細胞の遊走は、c-Srcで活性化された全長型Cav1.2 C末端を有するL型Ca2+チャネルを介する）. 信州大学, 2017, 博士論文.", "subitem_description_type": "Other"}]}, "item_14_dissertation_number_7": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "13601甲第1125号"}]}, "item_14_link_74": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000431873800005"}]}, "item_14_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "信州大学"}]}, "item_14_relation_54": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "29441404"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=29441404", "subitem_relation_type_select": "PMID"}}]}, "item_14_relation_55": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1007/s00424-018-2114-3"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1007/s00424-018-2114-3", "subitem_relation_type_select": "DOI"}}]}, "item_14_rights_69": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "The final publication is available at link.springer.com"}]}, "item_14_select_71": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_14_text_11": {"attribute_name": "学位の区分", "attribute_value_mlt": [{"subitem_text_value": "Doctoral"}]}, "item_14_text_12": {"attribute_name": "学位の分野", "attribute_value_mlt": [{"subitem_text_value": "医学"}]}, "item_14_text_13": {"attribute_name": "学位の報告番号", "attribute_value_mlt": [{"subitem_text_value": "甲第1125号"}]}, "item_14_text_76": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Vascular smooth muscle cells; Platelet-derived growth factor; Ca(V)1.2 channels; c-Src"}]}, "item_14_text_77": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "HIGH BLOOD-PRESSURE; CALCIUM-CHANNELS; SUBUNIT; KINASE; TRANSCRIPTION; CHEMOTAXIS; MECHANISMS; ARTERIES; DOMAIN"}]}, "item_14_textarea_75": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "In atherosclerosis, vascular smooth muscle cells (VSMC) migrate from the media toward the intima of the arteries in response to cytokines, such as platelet-derived growth factor (PDGF). However, molecular mechanism underlying the PDGF-induced migration of VSMCs remains unclear. The migration of rat aorta-derived synthetic VSMCs, A7r5, in response to PDGF was potently inhibited by a Ca(V)1.2 channel inhibitor, nifedipine, and a Src family tyrosine kinase (SFK)/Abl inhibitor, bosutinib, in a less-than-additive manner. PDGF significantly increased Ca(V)1.2 channel currents without altering Ca(V)1.2 protein expression levels in A7r5 cells. This reaction was inhibited by C-terminal Src kinase, a selective inhibitor of SFKs. In contractile VSMCs, the C-terminus of Ca(V)1.2 is proteolytically cleaved into proximal and distal C-termini (PCT and DCT, respectively). Clipped DCT is noncovalently reassociated with PCT to autoinhibit the channel activity. Conversely, in synthetic A7r5 cells, full-length Ca(V)1.2 (Ca(V)1.2FL) is expressed much more abundantly than truncated Ca(V)1.2. In a heterologous expression system, c-Src activated Ca(V)1.2 channels composed of Ca(V)1.2FL but not truncated Ca(V)1.2 (Ca(V)1.2 Delta 1763) or Ca(V)1.2 Delta 1763 plus clipped DCT. Further, c-Src enhanced the coupling efficiency between the voltage-sensing domain and activation gate of Ca(V)1.2FL channels by phosphorylating Tyr1709 and Tyr1758 in PCT. Compared with Ca(V)1.2 Delta 1763, c-Src could more efficiently bind to and phosphorylate Ca(V)1.2FL irrespective of the presence or absence of clipped DCT. Therefore, in atherosclerotic lesions, phenotypic switching of VSMCs may facilitate pro-migratory effects of PDGF on VSMCs by suppressing posttranslational Ca(V)1.2 modifications."}]}, "item_1627890986942": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_be7fb7dd8ff6fe43", "subitem_version_type": "NA"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "郭, 暁光"}], "nameIdentifiers": [{"nameIdentifier": "105923", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-06-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "13MH205F_yoshi.pdf", "filesize": [{"value": "123.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 123800.0, "url": {"label": "内容の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/19970/files/13MH205F_yoshi.pdf"}, "version_id": "a6c1d2d3-d06a-48c3-b44d-6d118a852117"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-06-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "13MH205F_shinsa.pdf", "filesize": [{"value": "134.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 134800.0, "url": {"label": "審査結果の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/19970/files/13MH205F_shinsa.pdf"}, "version_id": "75fc24e7-530a-4287-9b2d-060eb599026f"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-02-13"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "13MH205F_ronbun.pdf", "filesize": [{"value": "1.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "The final publication is available at link.springer.com", "licensetype": "license_note", "mimetype": "application/pdf", "size": 1700000.0, "url": {"label": "博士論文の全文", "url": "https://soar-ir.repo.nii.ac.jp/record/19970/files/13MH205F_ronbun.pdf"}, "version_id": "c5f5b549-686c-4c57-b40d-400be78b744c"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "PDGF-induced migration of synthetic vascular smooth muscle cells through c-Src-activated L-type Ca2+ channels with full-length CaV1.2 C-terminus（血小板由来増殖因子による合成型血管平滑筋細胞の遊走は、c-Srcで活性化された全長型Cav1.2 C末端を有するL型Ca2+チャネルを介する）", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "PDGF-induced migration of synthetic vascular smooth muscle cells through c-Src-activated L-type Ca2+ channels with full-length CaV1.2 C-terminus（血小板由来増殖因子による合成型血管平滑筋細胞の遊走は、c-Srcで活性化された全長型Cav1.2 C末端を有するL型Ca2+チャネルを介する）", "subitem_title_language": "en"}]}, "item_type_id": "14", "owner": "1", "path": ["849"], "permalink_uri": "http://hdl.handle.net/10091/00020731", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2018-06-28"}, "publish_date": "2018-06-28", "publish_status": "0", "recid": "19970", "relation": {}, "relation_version_is_last": true, "title": ["PDGF-induced migration of synthetic vascular smooth muscle cells through c-Src-activated L-type Ca2+ channels with full-length CaV1.2 C-terminus（血小板由来増殖因子による合成型血管平滑筋細胞の遊走は、c-Srcで活性化された全長型Cav1.2 C末端を有するL型Ca2+チャネルを介する）"], "weko_shared_id": -1}