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Fascin1 supresses RIG-I-like receptor signaling and interferon-\u03b2 production by associating with I\u03baB kinase \u03b5(IKK\u03b5) in colon cancer\uff08RIG-I\u7d4c\u8def\u3092\u4ecb\u3057\u305fFascin1\u306b\u3088\u308bIFN-\u03b2\u6291\u5236\u5236\u5fa1\uff09. \u4fe1\u5dde\u5927\u5b66, 2018, \u535a\u58eb\u8ad6\u6587.", "subitem_description_type": "Other"}]}, "item_14_dissertation_number_7": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7", "attribute_value_mlt": [{"subitem_dissertationnumber": "13601\u7532\u7b2c1133\u53f7"}]}, "item_14_link_74": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000431108600009"}]}, "item_14_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u4fe1\u5dde\u5927\u5b66"}]}, "item_14_relation_54": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "29496994"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=29496994", "subitem_relation_type_select": "PMID"}}]}, "item_14_relation_55": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1074/jbc.M117.819201"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1074/jbc.M117.819201", "subitem_relation_type_select": "DOI"}}]}, "item_14_rights_69": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "This research was originally published in J. Biol. Chem. Tomio Matsumura, Shigeaki Hida, Masato Kitazawa, Chifumi Fujii, Akira Kobayashi, Michiko Takeoka, Shun-ichiro Taniguchi, Shin-ichi Miyagawa. Fascin1 suppresses RIG-I\u2013like receptor signaling and interferon-\u03b2 production by associating with I\u03baB kinase \u03f5 (IKK\u03f5) in colon cancer. J. Biol. Chem. 2018; Vol. 293:6326. \u00a9 the American Society for Biochemistry and Molecular Biology. 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Because viral infection has been shown to activate immune cells and to induce interferon-beta expression in human cancer cells, we evaluated the effects of fascin1 on virus-dependent signaling via the membrane- and actin-associated protein RIG-I (retinoic acid-inducible gene I) in colon cancer cells. We knocked down fascin1 expression with shRNA retrovirally transduced into a DLD-1 colon cancer and L929 fibroblast-like cell lines and used luciferase reporter assays and co-immunoprecipitation to identify fascin1 targets. We found that intracellular poly(I.C) transfection to mimic viral infection enhances the RIG-I/MDA5 (melanoma differentiation-associated gene 5)-mediated dimerization of interferon regulatory factor 3 (IRF-3). The transfection also significantly increased the expression levels of IRF-7, interferon-beta, and interferon-inducible cytokine IP-10 in fascin1-deleted cells compared with controls while significantly suppressing cell growth, migration, and invasion. We also found that fascin1 constitutively interacts with I kappa B kinase epsilon (IKK epsilon) in the RIG-I signaling pathway. In summary, we have identified fascin1 as a suppressor of the RIG-I signaling pathway associating with I kappa B kinase epsilon in DLD-1 colon cancer cells to suppress immune responses to viral infection."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u677e\u6751, \u5bcc\u7a42"}], "nameIdentifiers": [{"nameIdentifier": "105929", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-06-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "09MH203C_yoshi.pdf", "filesize": [{"value": "139.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 139000.0, "url": {"label": "\u5185\u5bb9\u306e\u8981\u65e8", "url": "https://soar-ir.repo.nii.ac.jp/record/19976/files/09MH203C_yoshi.pdf"}, "version_id": "00d6627b-353d-48fc-9d1b-414c7bcfa32d"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-06-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "09MH203C_shinsa.pdf", "filesize": [{"value": "100.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 100700.0, "url": {"label": "\u5be9\u67fb\u7d50\u679c\u306e\u8981\u65e8", "url": "https://soar-ir.repo.nii.ac.jp/record/19976/files/09MH203C_shinsa.pdf"}, "version_id": "39b31b09-7e16-4c3c-92b1-eaff56fa587d"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-03-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "09MH203C_ronbun.pdf", "filesize": [{"value": "760.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "This research was originally published in J. Biol. Chem. Tomio Matsumura, Shigeaki Hida, Masato Kitazawa, Chifumi Fujii, Akira Kobayashi, Michiko Takeoka, Shun-ichiro Taniguchi, Shin-ichi Miyagawa. Fascin1 suppresses RIG-I\u2013like receptor signaling and interferon-\u03b2 production by associating with I\u03baB kinase \u03f5 (IKK\u03f5) in colon cancer. J. Biol. 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