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In Vivo Roles of Fatty Acid Biosynthesis Enzymes in Biosynthesis of Biotin and alpha-Lipoic Acid in Corynebacterium glutamicum
http://hdl.handle.net/10091/00020750
http://hdl.handle.net/10091/00020750e05b8af1-8762-4e1d-b763-f9612c65f593
| 名前 / ファイル | ライセンス | アクション |
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In_vivo_roles_of_fatty_acid-biosynthetic_enzymes_in_biosynthesis_3_of_biotin.pdf (783.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-07-24 | |||||
| タイトル | ||||||
| タイトル | In Vivo Roles of Fatty Acid Biosynthesis Enzymes in Biosynthesis of Biotin and alpha-Lipoic Acid in Corynebacterium glutamicum | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1128/AEM.01322-17 | |||||
| 関連名称 | 10.1128/AEM.01322-17 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Corynebacterium glutamicum | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | biotin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | fatty acid biosynthesis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | lipoic acid | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | metabolic engineering | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Ikeda, Masato
× Ikeda, Masato× Nagashima, Takashi× Nakamura, Eri× Kato, Ryosuke× Ohshita, Masakazu× Hayashi, Mikiro× Takeno, Seiki |
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| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Ikeda, Masato | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html | |||||
| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Takeno, Seiki | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html | |||||
| 出版者 | ||||||
| 出版者 | AMER SOC MICROBIOLOGY | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 83(19):UNSP e01322-17 (2017) | |||||
| 書誌情報 |
APPLIED AND ENVIRONMENTAL MICROBIOLOGY 巻 83, 号 19, p. UNSP e01322-17, 発行日 2017-10 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | For fatty acid biosynthesis, Corynebacterium glutamicum uses two type I fatty acid synthases (FAS-I), FasA and FasB, in addition to acetyl-coenzyme A (CoA) carboxylase (ACC) consisting of AccBC, AccD1, and AccE. The in vivo roles of the enzymes in supplying precursors for biotin and alpha-lipoic acid remain unclear. Here, we report genetic evidence demonstrating that the biosynthesis of these cofactors is linked to fatty acid biosynthesis through the FAS-I pathway. For this study, we used wild-type C. glutamicum and its derived biotin vitamer producer BFI-5, which was engineered to express Escherichia coli bioBF and Bacillus subtilis bioI. Disruption of either fasA or fasB in strain BFI-5 led to decreased production of biotin vitamers, whereas its amplification contributed to increased production, with a larger impact of fasA in both cases. Double disruptions of fasA and fasB resulted in no biotin vitamer production. The acc genes showed a positive effect on production when amplified simultaneously. Augmented fatty acid biosynthesis was also reflected in pimelic acid production when carbon flow was blocked at the BioF reaction. These results indicate that carbon flow down the FAS-I pathway is destined for channeling into the biotin biosynthesis pathway, and that FasA in particular has a significant impact on precursor supply. In contrast, fasB disruption resulted in auxotrophy for lipoic acid or its precursor octanoic acid in both wild-type and BFI-5 strains. The phenotypes were fully complemented by plasmid-mediated expression of fasB but not fasA. These results reveal that FasB plays a specific physiological role in lipoic acid biosynthesis in C. glutamicum. IMPORTANCE For the de novo biosynthesis of fatty acids, C. glutamicum exceptionally uses a eukaryotic multifunctional type I fatty acid synthase (FAS-I) system comprising FasA and FasB, in contrast to most bacteria, such as E. coli and B. subtilis, which use an individual nonaggregating type II fatty acid synthase (FAS-II) system. In this study, we reported genetic evidence demonstrating that the FAS-I system is the source of the biotin precursor in vivo in the engineered biotin-prototrophic C. glutamicum strain. This study also uncovered the important physiological role of FasB in lipoic acid biosynthesis. Here, we present an FAS-I enzyme that functions in supplying the lipoic acid precursor, although its biosynthesis has been believed to exclusively depend on FAS-II in organisms. The findings obtained here provide new insights into the metabolic engineering of this industrially important microorganism to produce these compounds effectively. | |||||
| 資源タイプ（コンテンツの種類） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0099-2240 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00543249 | |||||
| PubMed | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/28754705/ | |||||
| 関連名称 | 28754705 | |||||
| 権利 | ||||||
| 権利情報 | Copyright © 2017 American Society for Microbiology. | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| WoS | ||||||
| 表示名 | Web of Science | |||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000412028500017 | |||||
