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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

In Vivo Roles of Fatty Acid Biosynthesis Enzymes in Biosynthesis of Biotin and alpha-Lipoic Acid in Corynebacterium glutamicum

http://hdl.handle.net/10091/00020750
http://hdl.handle.net/10091/00020750
e05b8af1-8762-4e1d-b763-f9612c65f593
名前 / ファイル ライセンス アクション
In_vivo_roles_of_fatty_acid-biosynthetic_enzymes_in_biosynthesis_3_of_biotin.pdf In_vivo_roles_of_fatty_acid-biosynthetic_enzymes_in_biosynthesis_3_of_biotin.pdf (783.2 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2018-07-24
タイトル
言語 en
タイトル In Vivo Roles of Fatty Acid Biosynthesis Enzymes in Biosynthesis of Biotin and alpha-Lipoic Acid in Corynebacterium glutamicum
言語
言語 eng
キーワード
主題Scheme Other
主題 Corynebacterium glutamicum
キーワード
主題Scheme Other
主題 biotin
キーワード
主題Scheme Other
主題 fatty acid biosynthesis
キーワード
主題Scheme Other
主題 lipoic acid
キーワード
主題Scheme Other
主題 metabolic engineering
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Ikeda, Masato

× Ikeda, Masato

WEKO 105946

Ikeda, Masato

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Nagashima, Takashi

× Nagashima, Takashi

WEKO 105947

Nagashima, Takashi

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Nakamura, Eri

× Nakamura, Eri

WEKO 105948

Nakamura, Eri

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Kato, Ryosuke

× Kato, Ryosuke

WEKO 105949

Kato, Ryosuke

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Ohshita, Masakazu

× Ohshita, Masakazu

WEKO 105950

Ohshita, Masakazu

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Hayashi, Mikiro

× Hayashi, Mikiro

WEKO 105951

Hayashi, Mikiro

Search repository
Takeno, Seiki

× Takeno, Seiki

WEKO 105952

Takeno, Seiki

Search repository
信州大学研究者総覧へのリンク
氏名 Ikeda, Masato
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html
信州大学研究者総覧へのリンク
氏名 Takeno, Seiki
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html
出版者
出版者 AMER SOC MICROBIOLOGY
引用
内容記述タイプ Other
内容記述 APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 83(19):UNSP e01322-17 (2017)
書誌情報 APPLIED AND ENVIRONMENTAL MICROBIOLOGY

巻 83, 号 19, p. UNSP e01322-17, 発行日 2017-10
抄録
内容記述タイプ Abstract
内容記述 For fatty acid biosynthesis, Corynebacterium glutamicum uses two type I fatty acid synthases (FAS-I), FasA and FasB, in addition to acetyl-coenzyme A (CoA) carboxylase (ACC) consisting of AccBC, AccD1, and AccE. The in vivo roles of the enzymes in supplying precursors for biotin and alpha-lipoic acid remain unclear. Here, we report genetic evidence demonstrating that the biosynthesis of these cofactors is linked to fatty acid biosynthesis through the FAS-I pathway. For this study, we used wild-type C. glutamicum and its derived biotin vitamer producer BFI-5, which was engineered to express Escherichia coli bioBF and Bacillus subtilis bioI. Disruption of either fasA or fasB in strain BFI-5 led to decreased production of biotin vitamers, whereas its amplification contributed to increased production, with a larger impact of fasA in both cases. Double disruptions of fasA and fasB resulted in no biotin vitamer production. The acc genes showed a positive effect on production when amplified simultaneously. Augmented fatty acid biosynthesis was also reflected in pimelic acid production when carbon flow was blocked at the BioF reaction. These results indicate that carbon flow down the FAS-I pathway is destined for channeling into the biotin biosynthesis pathway, and that FasA in particular has a significant impact on precursor supply. In contrast, fasB disruption resulted in auxotrophy for lipoic acid or its precursor octanoic acid in both wild-type and BFI-5 strains. The phenotypes were fully complemented by plasmid-mediated expression of fasB but not fasA. These results reveal that FasB plays a specific physiological role in lipoic acid biosynthesis in C. glutamicum. IMPORTANCE For the de novo biosynthesis of fatty acids, C. glutamicum exceptionally uses a eukaryotic multifunctional type I fatty acid synthase (FAS-I) system comprising FasA and FasB, in contrast to most bacteria, such as E. coli and B. subtilis, which use an individual nonaggregating type II fatty acid synthase (FAS-II) system. In this study, we reported genetic evidence demonstrating that the FAS-I system is the source of the biotin precursor in vivo in the engineered biotin-prototrophic C. glutamicum strain. This study also uncovered the important physiological role of FasB in lipoic acid biosynthesis. Here, we present an FAS-I enzyme that functions in supplying the lipoic acid precursor, although its biosynthesis has been believed to exclusively depend on FAS-II in organisms. The findings obtained here provide new insights into the metabolic engineering of this industrially important microorganism to produce these compounds effectively.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0099-2240
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00543249
PubMed
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/28754705/
関連名称 28754705
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1128/AEM.01322-17
関連名称 10.1128/AEM.01322-17
権利
権利情報 Copyright © 2017 American Society for Microbiology.
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000412028500017
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