{"_buckets": {"deposit": "dad10eb9-ddd5-42f4-9242-e7b433c40224"}, "_deposit": {"id": "19996", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "19996"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00019996", "sets": []}, "author_link": ["105965"], "item_14_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-03-31", "bibliographicIssueDateType": "Issued"}}]}, "item_14_date_granted_8": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2017-03-31"}]}, "item_14_degree_grantor_10": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "信州大学(Shinshu university)"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "13601", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_14_degree_name_9": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（医学）"}]}, "item_14_description_26": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "雑誌に発表。MOLECULAR THERAPY-METHODS \u0026 CLINICAL DEVELOPMENT. 8():131-140 (2018); doi:10.1016/j.omtm.2017.12.003.", "subitem_description_type": "Other"}]}, "item_14_description_37": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Thesis", "subitem_description_type": "Other"}]}, "item_14_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "盛田　大介. Enhanced expression of anti-CD19 chimeric antigen receptor in piggyBac transposon-engineered T cells（抗CD19キメラ抗原受容体の発現を増強させたpiggyBacトランスポゾン遺伝子改変T細胞）. 信州大学, 2017, 博士論文.", "subitem_description_type": "Other"}]}, "item_14_dissertation_number_7": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "13601甲第1130号"}]}, "item_14_link_74": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000432653500012"}]}, "item_14_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "信州大学"}]}, "item_14_relation_54": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "29687032"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/29687032/", "subitem_relation_type_select": "PMID"}}]}, "item_14_relation_55": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/j.omtm.2017.12.003"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.omtm.2017.12.003", "subitem_relation_type_select": "DOI"}}]}, "item_14_rights_69": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2017 The Authors. / This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)."}]}, "item_14_select_71": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_14_text_11": {"attribute_name": "学位の区分", "attribute_value_mlt": [{"subitem_text_value": "doctoral"}]}, "item_14_text_12": {"attribute_name": "学位の分野", "attribute_value_mlt": [{"subitem_text_value": "医学"}]}, "item_14_text_13": {"attribute_name": "学位の報告番号", "attribute_value_mlt": [{"subitem_text_value": "甲第1130号"}]}, "item_14_text_77": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "SLEEPING-BEAUTY; IN-VIVO; B-CELL; LYMPHOCYTES; IMMUNOTHERAPY; SPECIFICITY; LYMPHOMA; LEUKEMIA; SYSTEM; CD8(+)"}]}, "item_14_textarea_75": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Adoptive T cell therapy using chimeric antigen receptor (CAR)-modified T cells is a promising cancer immunotherapy. We previously developed a non-viral method of gene transfer into T cells using a piggyBac transposon system to improve the cost-effectiveness of CAR-T cell therapy. Here, we have further improved our technology by a novel culture strategy to increase the transfection efficiency and to reduce the time of T cell manufacturing. Using a CH2CH3-free CD19-specific CAR transposon vector and combining irradiated activated T cells (ATCs) as feeder cells and virus-specific T cell receptor (TCR) stimulation, we achieved 51.4% +/- 14% CAR(+) T cells and 2.8-fold expansion after 14 culture days. Expanded CD19.CAR-T cells maintained a significant fraction of CD45RA(+) CCR7(+) T cells and demonstrated potent antitumor activity against CD19(+) leukemic cells both in vitro and in vivo. Therefore, piggyBac-based gene transfer may provide an alternative to viral gene transfer for CAR-T cell therapy."}]}, "item_1627890986942": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_be7fb7dd8ff6fe43", "subitem_version_type": "NA"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "盛田, 大介", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "105965", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-08-09"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "13MH043F_yoshi.pdf", "filesize": [{"value": "179.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 179800.0, "url": {"label": "内容の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/19996/files/13MH043F_yoshi.pdf"}, "version_id": "395d0e99-68b4-437e-b500-d9c79dc5cd0c"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-08-09"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "13MH043F_shinsa.pdf", "filesize": [{"value": "105.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 105400.0, "url": {"label": "審査結果の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/19996/files/13MH043F_shinsa.pdf"}, "version_id": "f2501fb7-d403-42ac-9006-73f511f36651"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-08-09"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "13MH043F_ronbun.pdf", "filesize": [{"value": "4.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "© 2017 The Authors. / This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).", "licensetype": "license_note", "mimetype": "application/pdf", "size": 4200000.0, "url": {"label": "博士論文の全文", "objectType": "fulltext", "url": "https://soar-ir.repo.nii.ac.jp/record/19996/files/13MH043F_ronbun.pdf"}, "version_id": "84fc2190-505f-48c3-98b2-494ce9927fe0"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Enhanced expression of anti-CD19 chimeric antigen receptor in piggyBac transposon-engineered T cells（抗CD19キメラ抗原受容体の発現を増強させたpiggyBacトランスポゾン遺伝子改変T細胞）", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Enhanced expression of anti-CD19 chimeric antigen receptor in piggyBac transposon-engineered T cells（抗CD19キメラ抗原受容体の発現を増強させたpiggyBacトランスポゾン遺伝子改変T細胞）", "subitem_title_language": "en"}]}, "item_type_id": "14", "owner": "1", "path": ["849"], "permalink_uri": "http://hdl.handle.net/10091/00020757", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2018-06-27"}, "publish_date": "2018-06-27", "publish_status": "0", "recid": "19996", "relation": {}, "relation_version_is_last": true, "title": ["Enhanced expression of anti-CD19 chimeric antigen receptor in piggyBac transposon-engineered T cells（抗CD19キメラ抗原受容体の発現を増強させたpiggyBacトランスポゾン遺伝子改変T細胞）"], "weko_shared_id": -1}