| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-01-20 |
| タイトル |
|
|
タイトル |
Intracellular cAMP contents regulate NAMPT expression via induction of C/EBPβ in adipocytes |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.bbrc.2019.11.165 |
|
|
関連名称 |
10.1016/j.bbrc.2019.11.165 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
adipogenesis |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
cAMP |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
CCAAT-enhancer-binding protein β |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
nicotinamide adenine dinucleotide |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
nicotinamide phosphoribosyltransferase |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Mitani, Takakazu
Watanabe, Shun
Wada, Kenjiro
Fujii, Hiroshi
Nakamura, Soichiro
Katayama, Shigeru
|
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
三谷, 塁一 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.ypcCPUkh.html |
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
藤井, 博 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.WhymjeSh.html |
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
中村, 宗一郎 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.OpyhuNDp.html |
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
片山, 茂 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.HFkaPUkh.html |
| 出版者 |
|
|
出版者 |
Elsevier |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Biochemical and Biophysical Research Communications. 522(3): 770-775. (2020) |
| 書誌情報 |
Biochemical and Biophysical Research Communications
巻 522,
号 3,
p. 770-775,
発行日 2020-02-12
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
A decline in intracellular nicotinamide adenine mononucleotide (NAD+) causes adipose tissue dysfunction. Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the rate-limiting step in the NAD+ biosynthesis pathway. However, the molecular mechanism that mediates regulation of NAMPT expression in adipocytes is yet to be elucidated. This study found that intracellular cAMP regulates NAMPT expression and promoter activity in 3T3-L1 adipocytes. cAMP-mediated Nampt promoter activity was suppressed by protein kinase A inhibitor H89, whereas AMP-activated protein kinase inhibitor compound C did not affect cAMP-mediated Nampt promoter activity. Intracellular cAMP induced CCAAT/enhancer-binding protein β (C/EBPβ) expression. Knockdown of C/EBPβ suppressed NAMPT expression and promoter activity. Furthermore, the Nampt promoter was activated by C/EBPβ, while LIP activated the dominant-negative form of C/EBPβ. Promoter sequence analysis revealed that the region from -96 to -76 on Nampt was required for C/EBPβ-mediated promoter activity. Additionally, chromatin immunoprecipitation assay demonstrated that C/EBPβ was bound to the promoter sequences of Nampt. Finally, NAMPT inhibitor FK866 suppressed adipogenesis in 3T3-L1 cells, and this suppressive effect was restored by nicotinamide mononucleotide treatment. These findings showed that intracellular cAMP increased NAMPT levels by induction of C/EBPβ expression and indicated that the induction of NAMPT expression was important for adipogenesis. |
| 資源タイプ（コンテンツの種類） |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Article |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1090-2104 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11542044 |
| PubMed |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/31791580/ |
|
|
関連名称 |
31791580 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
18K14405_1.pdf
