{"_buckets": {"deposit": "6a7b5f82-6edc-451a-9fe0-14fe009852f0"}, "_deposit": {"id": "2000703", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2000703"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:02000703", "sets": ["1017"]}, "author_link": [], "control_number": "2000703", "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-12-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "338", "bibliographicPageStart": "331", "bibliographicVolumeNumber": "63", "bibliographic_titles": [{"bibliographic_title": "Journal of Nutritional Science and Vitaminology"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Caffeine is a methylxanthine derived from plant foods such as coffee beans and tea leaves, and has multiple biological activities against physiological response and several diseases. Although there are some reports about the direct effect of caffeine against anti-lipid accumulation in vitro, the effect of caffeine on lipid accumulation in adipocytes through stimulating intestinal epithelial cells is unknown. Since direct treatment with caffeine to 3T3-L1 cells did not affect lipid accumulation, we determined whether caffeine-stimulated intestinal epithelial Caco-2 cells influence the lipid accumulation in 3T3-L1 adipocytes. Caco-2 cells were cultured on a transwell insert with or without caffeine for 24 h. Subsequently, the basolateral component of the Caco-2 cell culture on the transwell was collected and termed caffeine-conditioning medium (CCM). When 3T3-L1 adipocytes were incubated with CCM, CCM decreased lipid accumulation and suppressed gene expression of proliferator activated receptor (PPAR) γ and CCAAT/enhancer binding protein (C/EBP) α in 3T3-L1 adipocytes. Furthermore, CCM decreased the expression of C/EBPβ and C/EBPδ at the protein level, but not at the mRNA level. We observed that a proteasome inhibitor, MG132, inhibited CCM-caused down-expression of C/EBPβ and C/EBPδ proteins, and that CCM promoted the ubiquitination level of C/EBPβ and C/EBPδ proteins. Protein microarray analysis showed caffeine suppresses the secretion of inflammatory cytokines, interleukin-8 and plasminogen activator inhibitor-1 from Caco-2 cells. These results suggest that caffeine indirectly suppresses lipid accumulation in 3T3-L1 adipocytes through decreasing secretion of inflammatory cytokines from Caco-2 cells.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Journal of Nutritional Science and Vitaminology. 63(5): 331-338. (2017)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "三谷, 塁一", "subitem_link_url": "https://soar-rd.shinshu-u.ac.jp/profile/ja.ypcCPUkh.html"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "一般財団法人 学会誌刊行センター"}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.3177/jnsv.63.331"}], "subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.3177/jnsv.63.331", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1881-7742", "subitem_source_identifier_type": "EISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1881-7742", "subitem_source_identifier_type": "EISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12108939", "subitem_source_identifier_type": "NCID"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mitani, Takakazu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nagano, Tomoya", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Harada, Kiyonori", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yamashita, Yoko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Ashida, Hitoshi", "creatorNameLang": "en"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-01-27"}], "download_preview_message": "", "file_order": 0, "filename": "JNSV63-5-331.pdf", "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 0, "url": {"label": "JNSV63-5-331.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/2000703/files/JNSV63-5-331.pdf"}, "version_id": "bf3a07b6-2179-4fee-8683-ee49f40d5dd8"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "caffeine", "subitem_subject_scheme": "Other"}, {"subitem_subject": "adipogenesis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "3T3-L1 adipocytes", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Caco-2 cells", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Caffeine-Stimulated Intestinal Epithelial Cells Suppress Lipid Accumulation in Adipocytes", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Caffeine-Stimulated Intestinal Epithelial Cells Suppress Lipid Accumulation in Adipocytes", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1017"], "permalink_uri": "http://hdl.handle.net/10091/0002000703", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-01-27"}, "publish_date": "2022-01-27", "publish_status": "0", "recid": "2000703", "relation": {}, "relation_version_is_last": true, "title": ["Caffeine-Stimulated Intestinal Epithelial Cells Suppress Lipid Accumulation in Adipocytes"], "weko_shared_id": -1}