Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2022-02-04 |
タイトル |
|
|
タイトル |
Genistein regulates adipogenesis by blocking the function of adenine nucleotide translocase-2 in the mitochondria |
言語 |
|
|
言語 |
eng |
DOI |
|
|
関連タイプ |
isVersionOf |
|
|
関連識別子 |
https://doi.org/10.1093/bbb/zbab203 |
|
|
関連名称 |
10.1093/bbb/zbab203 |
キーワード |
|
|
主題 |
adipogenesis, AMPK, ANT2, genistein, target molecule |
資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
著者 |
Ikeda, Takahiro
Watanabe, Shun
Mitani, Takakazu
|
信州大学研究者総覧へのリンク |
|
|
氏名 |
三谷, 塁一 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.ypcCPUkh.html |
出版者 |
|
|
出版者 |
Oxford University Press |
引用 |
|
|
内容記述 |
Bioscience, biotechnology, and biochemistry. 86(2): 260-272 (2022) |
書誌情報 |
Bioscience, biotechnology, and biochemistry.
巻 86,
号 2,
p. 260-272,
発行日 2022-02
|
抄録 |
|
|
内容記述 |
Genistein exerts antiadipogenic effects, but its target molecules remain unclear. Here, we delineated the molecular mechanism underlying the antiadipogenic effect of genistein. A pulldown assay using genistein-immobilized beads identified adenine nucleotide translocase-2 as a genistein-binding protein in adipocytes. Adenine nucleotide translocase-2 exchanges ADP/ATP through the mitochondrial inner membrane. Similar to the knockdown of adenine nucleotide translocase-2, genistein treatment decreased ADP uptake into the mitochondria and ATP synthesis. Genistein treatment and adenine nucleotide translocase-2 knockdown suppressed adipogenesis and increased phosphorylation of AMP-activated protein kinase. Adenine nucleotide translocase-2 knockdown reduced the transcriptional activity of CCAAT/enhancer-binding protein β, whereas AMP-activated protein kinase inhibition restored the suppression of adipogenesis by adenine nucleotide translocase-2 knockdown. These results indicate that genistein interacts directly with adenine nucleotide translocase-2 to suppress its function. The downregulation of adenine nucleotide translocase-2 reduces the transcriptional activity of CCAAT/enhancer-binding protein β via activation of AMP-activated protein kinase, which consequently represses adipogenesis. |
資源タイプ(コンテンツの種類) |
|
|
内容記述 |
Article |
ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1347-6947 |
書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12018610 |
PubMed |
|
|
|
関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/34849563/ |
|
|
関連名称 |
34849563 |
出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |