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Identification of a novel osteogenetic oligodeoxynucleotide (osteoDN) that promotes osteoblast differentiation in a TLR9-independent manner.
http://hdl.handle.net/10091/0002000896
http://hdl.handle.net/10091/00020008966e1f6384-be26-4d92-9b86-2d4b8cbb4df8
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Nanomaterials_12-1680.pdf
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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| 公開日 | 2022-05-16 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Identification of a novel osteogenetic oligodeoxynucleotide (osteoDN) that promotes osteoblast differentiation in a TLR9-independent manner. | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| DOI | ||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||
| 関連識別子 | https://doi.org/10.3390/nano12101680 | |||||||||||||||
| 関連名称 | 10.3390/nano12101680 | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題 | osteogenetic oligodeoxynucleotide (osteoDN), osteoblast, bone differentiation, calcification, mineralization, Toll-like receptor 9 (TLR9) | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| タイプ | journal article | |||||||||||||||
| 著者 |
Nihashi, Yuma
× Nihashi, Yuma
× Miyoshi, Mana
× Umezawa, Koji
× Shimosato, Takeshi
× Takaya, Tomohide
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| 信州大学研究者総覧へのリンク | ||||||||||||||||
| 氏名 | 梅澤, 公二 | |||||||||||||||
| URL | https://soar-rd.shinshu-u.ac.jp/profile/ja.yVcFPhkh.html | |||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||
| 氏名 | 下里, 剛士 | |||||||||||||||
| URL | https://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html | |||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||
| 氏名 | 高谷, 智英 | |||||||||||||||
| URL | https://soar-rd.shinshu-u.ac.jp/profile/ja.yUcePUkh.html | |||||||||||||||
| 出版者 | ||||||||||||||||
| 出版者 | MDPI | |||||||||||||||
| 引用 | ||||||||||||||||
| 内容記述 | Nanomaterials. 12(10) : 1680 (2022) | |||||||||||||||
| 書誌情報 |
en : Nanomaterials 巻 12, 号 10, p. 1680, 発行日 2022-05-14 |
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| 抄録 | ||||||||||||||||
| 内容記述 | Dysfunction of bone-forming cells, osteoblasts, is one of the causes of osteoporosis. Accumulating evidence has indicated that oligodeoxynucleotides (ODNs) designed from genome sequences have the potential to regulate osteogenic cell fate. Such osteogenetic ODNs (osteoDNs) targeting and activating osteoblasts can be the candidates of nucleic acid drugs for osteoporosis. In this study, the ODN library derived from the Lacticaseibacillus rhamnosus GG genome was screened to determine its osteogenetic effect on murine osteoblast cell line MC3T3-E1. An 18-base ODN, iSN40, was identified to enhance alkaline phosphatase activity of osteoblasts within 48 h. iSN40 also induced the expression of osteogenic genes such as Msx2, osterix, collagen type 1, osteopontin, and osteocalcin. Eventually, iSN40 facilitated calcium deposition on osteoblasts at the late stage of differentiation. Intriguingly, the CpG motif within iSN40 was not required for its osteogenetic activity, indicating that iSN40 functions in a TLR9-independent manner. These data demonstrate that iSN40 serves as a novel osteogenetic ODN (osteoDN) that promotes osteoblast differentiation. iSN40 provides a potential seed of the nucleic acid drug that activating osteoblasts for osteoporosis therapy. | |||||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||||
| ISSN | ||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||
| 収録物識別子 | 2079-4991 | |||||||||||||||
| 権利 | ||||||||||||||||
| 権利情報Resource | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | |||||||||||||||
| 出版タイプ | ||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||
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Nihashi, Yuma, Miyoshi, Mana, Umezawa, Koji, Shimosato, Takeshi, Takaya, Tomohide, 2022, Identification of a novel osteogenetic oligodeoxynucleotide (osteoDN) that promotes osteoblast differentiation in a TLR9-independent manner.: MDPI, 1680– p.
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