| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-09-12 |
| タイトル |
|
|
タイトル |
Production of C-glucosides of flavonoids and related compounds by Escherichia coli expressing buckwheat C-glucosyltransferase |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.5511/plantbiotechnology.14.1016a |
|
|
関連名称 |
10.5511/plantbiotechnology.14.1016a |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
flavonoid C-glucoside |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
bioconversion in E. coli |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
C-glucoside production |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
C-glucosyltransferase |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
biocatalyst |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Ito, Takamitsu
Fujimoto, Shunsuke
Shimosaka, Makoto
Taguchi, Goro
|
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
下坂, 誠 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html |
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
田口, 悟朗 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html |
| 出版者 |
|
|
出版者 |
日本植物バイオテクノロジー学会 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Plant Biotechnology. 31(5): 519-524 (2014) |
| 書誌情報 |
Plant Biotechnology
巻 31,
号 5,
p. 519-524,
発行日 2014-12-15
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
C-Glucosides are glucose-containing glycosides that have carbon–carbon bonds between the anomeric carbon of the sugar moieties and aglycon, rendering the molecules remarkably stable against hydrolysis by enzymes or acids. In this work, we showed the production of C-glucosides of flavonoids and related compounds (i.e., 2-hydroxyflavanone, dihydrochalcone, and trihydroxyacetophenone) by Escherichia coli expressing buckwheat C-glucosyltransferase. The substrates in their respective cultures were taken up by the cells and C-glucosylated, and the products were released into the culture media. The bioconversion process was completed in 1–2 h, but products were already observed immediately after addition of the substrates (200 µM). The conversion rates of these substrates reached 80–95%. Without addition of glucose to the conversion media, almost no C-glucosides were produced. Although the amounts of the substrates fed to their respective cultures were limited by their solubility in water, repeated addition of the substrate to the culture at regular time intervals effectively increased the total amount of product obtained. |
|
言語 |
en |
| 資源タイプ（コンテンツの種類） |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Article |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1347-6114 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11250821 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
PlantBiotechnol31-519.pdf
