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  1. 080 繊維学部
  2. 0801 学術論文

Production of C-glucosides of flavonoids and related compounds by Escherichia coli expressing buckwheat C-glucosyltransferase

http://hdl.handle.net/10091/0002001060
http://hdl.handle.net/10091/0002001060
00358ccb-80ea-4535-9d1f-5c661616641e
名前 / ファイル ライセンス アクション
PlantBiotechnol31-519.pdf PlantBiotechnol31-519.pdf
Item type 学術雑誌論文 / Journal Article(1)
公開日 2022-09-12
タイトル
タイトル Production of C-glucosides of flavonoids and related compounds by Escherichia coli expressing buckwheat C-glucosyltransferase
言語
言語 eng
DOI
関連タイプ isVersionOf
関連識別子 https://doi.org/10.5511/plantbiotechnology.14.1016a
関連名称 10.5511/plantbiotechnology.14.1016a
キーワード
主題 flavonoid C-glucoside, bioconversion in E. coli, C-glucoside production, C-glucosyltransferase, biocatalyst
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Ito, Takamitsu

× Ito, Takamitsu

en Ito, Takamitsu

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Fujimoto, Shunsuke

× Fujimoto, Shunsuke

en Fujimoto, Shunsuke

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Shimosaka, Makoto

× Shimosaka, Makoto

en Shimosaka, Makoto

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Taguchi, Goro

× Taguchi, Goro

en Taguchi, Goro

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信州大学研究者総覧へのリンク
氏名 下坂, 誠
URL https://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html
信州大学研究者総覧へのリンク
氏名 田口, 悟朗
URL https://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html
出版者
出版者 日本植物バイオテクノロジー学会
引用
内容記述 Plant Biotechnology. 31(5): 519-524 (2014)
書誌情報 Plant Biotechnology

巻 31, 号 5, p. 519-524, 発行日 2014-12-15
抄録
内容記述 C-Glucosides are glucose-containing glycosides that have carbon–carbon bonds between the anomeric carbon of the sugar moieties and aglycon, rendering the molecules remarkably stable against hydrolysis by enzymes or acids. In this work, we showed the production of C-glucosides of flavonoids and related compounds (i.e., 2-hydroxyflavanone, dihydrochalcone, and trihydroxyacetophenone) by Escherichia coli expressing buckwheat C-glucosyltransferase. The substrates in their respective cultures were taken up by the cells and C-glucosylated, and the products were released into the culture media. The bioconversion process was completed in 1–2 h, but products were already observed immediately after addition of the substrates (200 µM). The conversion rates of these substrates reached 80–95%. Without addition of glucose to the conversion media, almost no C-glucosides were produced. Although the amounts of the substrates fed to their respective cultures were limited by their solubility in water, repeated addition of the substrate to the culture at regular time intervals effectively increased the total amount of product obtained.
資源タイプ(コンテンツの種類)
ISSN
収録物識別子タイプ EISSN
収録物識別子 1347-6114
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11250821
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
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Cite as

Ito, Takamitsu, Fujimoto, Shunsuke, Shimosaka, Makoto, Taguchi, Goro, 2014, Production of C-glucosides of flavonoids and related compounds by Escherichia coli expressing buckwheat C-glucosyltransferase: 日本植物バイオテクノロジー学会, 519–524 p.

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