| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-06-13 |
| タイトル |
|
|
タイトル |
Development of Bilayered Bone Marrow-derived Cell-Gelatin Grafts for Augmentation Cystoplasty and Reconstruction of Bladder Tissues in Rats |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.11441/shinshumedj.71.167 |
|
|
関連名称 |
10.11441/shinshumedj.71.167 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
bladder augmentation cystoplasty |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
bone marrow-derived mesenchymal cell |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
gelatin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
bladder tissue |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
rat |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Inoue, Yoshihiro
Imamura, Tetsuya
Ogawa, Noriyuki
Hara, Hiroaki
Matsumoto, Yuki
Ueno, Manabu
Minagawa, Tomonori
Ogawa, Teruyuki
Ishizuka, Osamu
|
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
今村, 哲也 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.ypACOmyC.html |
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
小川, 典之 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.yVTVPaym.html |
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
原, 寛彰 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.OpcNPUym.html |
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
上野, 学 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.yUkmbVym.html |
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
皆川, 倫範 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.jCfagUkh.html |
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
小川, 輝之 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.yhTUgpkh.html |
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
石塚, 修 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.gmfaHekh.html |
| 出版者 |
|
|
出版者 |
信州医学会 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
信州医学雑誌 71(3) : 167-177, (2023) |
| 書誌情報 |
信州医学雑誌
巻 71,
号 3,
p. 167-177,
発行日 2023-06-10
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| 抄録 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
Background : This study attempted to produce a novel graft composed of bone marrow-derived mesenchymal cell (BMC) layer-gelatin sheets for bladder augmentation cystoplasty. Then, we determined if the grafts could reconstruct bladder tissues. Methods : BMCs harvested from the femurs of green fluorescence protein (GFP)-transfected Sprague-Dawley (SD) rats were adherent and proliferating cells on collagen dishes. The cells were then cultured on temperatureresponsive culture dishes. Following this, the BMCs maintaining cell-cell contacts within the monolayer itself were applied to a gelatin sheet. Two BMC layer-gelatin sheets were overlaid together with the cell sides juxtaposed with one another (bilayered BMC-gelatin graft). Bladder top of SD rats were incised and transplanted with the bilayered BMC-gelatin grafts. Similarly, urinary bladders irradiated with 2 Gy once a week for 5 weeks were also conducted. As control, bilayered acellular-gelatin grafts were used. At 4 weeks after transplantation, the bladders were histologically investigated. Results : At 4 weeks after transplantation into either normal or radiation-injured urinary bladders, incised regions closed. The closed regions of bladder top had reconstructed tissues that were formed with urothelium, and smooth muscle layers. Within the reconstructed tissues, the thickness of the smooth muscle layers in the bilayered BMC-gelatin graft-transplanted bladders were larger compared to controls. The GFP-positive transplanted BMCs were detected. Some of the cells were simultaneously positive for smooth muscle or nerve cell markers. Conclusion : This study showed that the bilayered BMC-gelatin grafts that were experimentally produced could reconstruct bladder tissues. The grafts would be developed as grafts for bladder augmentation cystoplasty. |
| 資源タイプ（コンテンツの種類） |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Article |
| ISSN |
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|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0037-3826 |
| 書誌レコードID |
|
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00120815 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
shinshuishi71-3_4.pdf
