{"_buckets": {"deposit": "a208ed79-1cb3-4b7f-84dd-a7511ebf057f"}, "_deposit": {"id": "20084", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "20084"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00020084"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-05-27", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageStart": "e0123971", "bibliographicVolumeNumber": "10", "bibliographic_titles": [{"bibliographic_title": "PLOS ONE"}]}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Late-outgrowth endothelial progenitor cells (EPCs) are stress-resistant and responsible for reparative functions in the cardiovascular system. Oxidized-LDL (oxLDL) plays a critical role in cardiovascular disease pathogenesis. However, it is largely unknown what the impacts of oxLDL are on late-outgrowth EPCs. This study aimed to investigate the concentration-related effects of oxLDL on EPC functions and related angiogenesis, in vitro and in vivo. In this study, early and late-outgrowth EPCs were generated from circulating human mononuclear cells. oxLDL may regulate EPC vasculogenic function via the lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1). Lower concentrations (5 \u03bcg/mL) of oxLDL can potentiate EPC tube formation in vitro and in vivo by activating eNOS mechanisms, which are mediated by p38 MAPK- and SAPK/JNK-related pathways. Higher concentrations of oxLDL (10-50 \u03bcg/mL) impaired EPC function via the activation of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase pathways and consequent inhibition of eNOS activity, which could be reversed by anti-oxidants (diphenylene iodonium and apocynin) and gp91phox siRNA. 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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited"}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1932-6203", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1932-6203", "subitem_source_identifier_type": "ISSN"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "CORONARY-ARTERY-DISEASE; CARDIOVASCULAR RISK-FACTORS; NITRIC-OXIDE SYNTHASE; OXIDATIVE STRESS; HEART-DISEASE; GENERAL-POPULATION; IN-VIVO; OX-LDL; PATHWAY; MORTALITY"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Late-outgrowth endothelial progenitor cells (EPCs) are stress-resistant and responsible for reparative functions in the cardiovascular system. Oxidized-LDL (oxLDL) plays a critical role in cardiovascular disease pathogenesis. However, it is largely unknown what the impacts of oxLDL are on late-outgrowth EPCs. This study aimed to investigate the concentration- related effects of oxLDL on EPC functions and related angiogenesis, in vitro and in vivo. In this study, early and late-outgrowth EPCs were generated from circulating human mononuclear cells. oxLDL may regulate EPC vasculogenic function via the lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1). Lower concentrations (5 mu g/mL) of oxLDL can potentiate EPC tube formation in vitro and in vivo by activating eNOS mechanisms, which are mediated by p38 MAPK-and SAPK/JNK-related pathways. Higher concentrations of oxLDL (10-50 mu g/mL) impaired EPC function via the activation of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase pathways and consequent inhibition of eNOS activity, which could be reversed by anti-oxidants (diphenylene iodonium and apocynin) and gp91(phox) siRNA. In conclusion, oxLDL has concentration-dependent biphasic effects on human late-outgrowth EPC tube formation in vitro and in vivo."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Lin, FY"}], "nameIdentifiers": [{"nameIdentifier": "106187", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsao, NW"}], "nameIdentifiers": [{"nameIdentifier": "106188", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shih, CM"}], "nameIdentifiers": [{"nameIdentifier": "106189", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Lin, YW"}], "nameIdentifiers": [{"nameIdentifier": "106190", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yeh, JS"}], "nameIdentifiers": [{"nameIdentifier": "106191", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Chen, JW"}], "nameIdentifiers": [{"nameIdentifier": "106192", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakagami, H"}], "nameIdentifiers": [{"nameIdentifier": "106193", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Morishita, R"}], "nameIdentifiers": [{"nameIdentifier": "106194", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sawamura, T"}], "nameIdentifiers": [{"nameIdentifier": "106195", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Huang, CY"}], "nameIdentifiers": [{"nameIdentifier": "106196", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-09-12"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "25293063_08.pdf", "filesize": [{"value": "4.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "\u00a9 2015 Lin et al. 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