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LOX-1 Plays an Important Role in Ischemia-Induced Angiogenesis of Limbs
http://hdl.handle.net/10091/00020848
http://hdl.handle.net/10091/00020848887da08c-062b-4b8e-8197-a0b6dd0e88a6
| 名前 / ファイル | ライセンス | アクション |
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25293063_15.pdf (731.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||
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| 公開日 | 2018-09-12 | |||||||||||||||||||||
| タイトル | ||||||||||||||||||||||
| タイトル | LOX-1 Plays an Important Role in Ischemia-Induced Angiogenesis of Limbs | |||||||||||||||||||||
| 言語 | ||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||
| DOI | ||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1371/journal.pone.0114542 | |||||||||||||||||||||
| 関連名称 | 10.1371/journal.pone.0114542 | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| タイプ | journal article | |||||||||||||||||||||
| 著者 |
Shiraki, T
× Shiraki, T
× Aoyama, T
× Yokoyama, C
× Hayakawa, Y
× Tanaka, T
× Nishigaki, K
× Sawamura, T
× Minatoguchi, S
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| 信州大学研究者総覧へのリンク | ||||||||||||||||||||||
| 氏名 | Sawamura, Tatsuya | |||||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HafpjeAF.html | |||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||
| 出版者 | PUBLIC LIBRARY SCIENCE | |||||||||||||||||||||
| 引用 | ||||||||||||||||||||||
| 内容記述 | PLOS ONE.9(12):e114542(2014) | |||||||||||||||||||||
| 書誌情報 |
PLOS ONE 巻 9, 号 12, p. e114542, 発行日 2014-12-16 |
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| 抄録 | ||||||||||||||||||||||
| 内容記述 | LOX-1, lectin-like oxidized low-density lipoprotein (LDL) receptor-1, is a single transmembrane receptor mainly expressed on endothelial cells. LOX-1 mediates the uptake of oxidized LDL, an early step in atherosclerosis; however, little is known about whether LOX-1 is involved in angiogenesis during tissue ischemia. Therefore, we examined the role of LOX-1 in ischemia-induced angiogenesis in the hindlimbs of LOX-1 knockout (KO) mice. Angiogenesis was evaluated in a surgically induced hindlimb ischemia model using laser Doppler blood flowmetry (LDBF) and histological capillary density (CD) and arteriole density (AD). After right hindlimb ischemia, the ischemic/nonischemic hindlimb blood flow ratio was persistently lower in LOX-1 KO mice than in wild-type (WT) mice. CD and AD were significantly smaller in LOX-1 KO mice than in WT mice on postoperative day 14. Immunohistochemical analysis revealed that the number of macrophages infiltrating ischemic tissues was significantly smaller in LOX-1 KO mice than in WT mice. The number of infiltrated macrophages expressing VEGF was also significantly smaller in LOX-1 KO mice than in WT mice. Western blot analysis and ROS production assay revealed that LOX- KO mice show significant decrease in Nox2 expression, ROS production and HIF-1α expression, the phosphorylation of p38 MAPK and NF-κB p65 subunit as well as expression of redox-sensitive vascular cell adhesion molecule-1 (VCAM-1) and LOX-1 itself in ischemic muscles, which is supposed to be required for macrophage infiltration expressing angiogenic factor VEGF. Reduction of VEGF expression successively suppressed the phosphorylation of Akt and eNOS, which accelerated angiogenesis, in the ischemic leg of LOX-1 KO mice. Our findings indicate that LOX-1 plays an important role in ischemia-induced angiogenesis by 1) Nox2-ROS-NF-κB activation, 2) upregulated expression of adhesion molecules: VCAM-1 and LOX-1 and 3) promoting macrophage infiltration, which expresses angiogenic factor VEGF. | |||||||||||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||||||||||
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| 収録物識別子タイプ | PISSN | |||||||||||||||||||||
| 収録物識別子 | 1932-6203 | |||||||||||||||||||||
| PubMed | ||||||||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||||||||
| 関連識別子 | https://www.ncbi.nlm.nih.gov/pubmed/25514797 | |||||||||||||||||||||
| 関連名称 | 25514797 | |||||||||||||||||||||
| 権利 | ||||||||||||||||||||||
| 権利情報 | © 2014 Shiraki et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||
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| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=6&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000349146300014 | |||||||||||||||||||||
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Shiraki, T, Aoyama, T, Yokoyama, C, Hayakawa, Y, Tanaka, T, Nishigaki, K, Sawamura, T, Minatoguchi, S, 2014, LOX-1 Plays an Important Role in Ischemia-Induced Angiogenesis of Limbs: PUBLIC LIBRARY SCIENCE, e114542– p.
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