{"_buckets": {"deposit": "03a66b41-d7d0-4f60-9bca-05d1f5d274aa"}, "_deposit": {"id": "20107", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "20107"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00020107", "sets": ["883"]}, "author_link": ["106326", "106327", "106328", "106329", "106330", "106331"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-09-01", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "93", "bibliographicPageStart": "87", "bibliographicVolumeNumber": "136", "bibliographic_titles": [{"bibliographic_title": "LIFE SCIENCES"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Aims\u003cbr/\u003eWe herein investigated the inducibility of cytochrome P450 1A1 (CYP1A1) by Δ9-tetrahydrocannabinol, cannabidiol (CBD), and cannabinol, three major phytocannabinoids, using human hepatoma HepG2 cells.\u003cbr/\u003eMain methods\u003cbr/\u003eThe expression of CYP1A1 and the aryl hydrocarbon receptor (AhR) was measured by a quantitative real-time polymerase chain reaction and/or Western blotting.\u003cbr/\u003eKey findings\u003cbr/\u003eΔ9-Tetrahydrocannabinol and CBD concentration-dependently induced the expression of CYP1A1 mRNA, whereas cannabinol showed little or no induction. Among the phytocannabinoids tested, CBD was the most potent inducer of CYP1A1 expression. The induction of CYP1A1 expression by CBD was significantly attenuated by the knockdown of AhR expression with AhR small interfering RNAs. The role of protein tyrosine kinases (PTKs) in the CBD-mediated induction of CYP1A1 was then examined using herbimycin A, a PTK inhibitor. The upregulation of CYP1A1 by CBD was significantly suppressed by herbimycin A as was the induction by omeprazole but not 3-methylcholanthrene. The inducibility of CYP1A1 by CBD-related compounds was examined to clarify the structural requirements for CBD-mediated CYP1A1 induction. Olivetol, which corresponds to the pentylresorcinol moiety of CBD, significantly induced the expression of CYP1A1, whereas d-limonene, CBD-2′-monomethyl ether, and CBD-2′,6′-dimethyl ether did not.\u003cbr/\u003eSignificance\u003cbr/\u003eThese results showed that CBD may have induced human CYP1A1 expression through the activation of PTK-dependent AhR signaling, in which two phenolic hydroxyl groups in the pentylresorcinol moiety of CBD may play structurally important roles.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "LIFE SCIENCES.136:87-93(2015)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "Yamaori, Satoshi", "subitem_link_url": "https://soar-rd.shinshu-u.ac.jp/profile/ja.jefNgUkh.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000361035500013"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "PERGAMON-ELSEVIER SCIENCE LTD"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "26187180"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://www.ncbi.nlm.nih.gov/pubmed/26187180", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/j.lfs.2015.07.007"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.lfs.2015.07.007", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0024-3205", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0024-3205", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00717011", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Marijuana; Cannabidiol; CYP1A1; Induction; AhR; Protein tyrosine kinase"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "PROTEIN-TYROSINE KINASES; AROMATIC-HYDROCARBONS; SIDESTREAM MARIJUANA; HABITUAL SMOKERS; AND/OR TOBACCO; DELTA(9)-TETRAHYDROCANNABINOL; CANNABINOIDS; MUTAGENICITY; INDUCTION; ACTIVATION"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Aims: We herein investigated the inducibility of cytochrome P450 1A1 (CYP1A1) by Delta(9)-tetrahydrocannabinol, cannabidiol (CBD), and cannabinol, three major phytocannabinoids, using human hepatoma HepG2 cells. \u003cbr/\u003eMain methods: The expression of CYP1A1 and the aryl hydrocarbon receptor (AhR) was measured by a quantitative real-time polymerase chain reaction and/or Western blotting. \u003cbr/\u003eKey findings:Delta(9)-Tetrahydrocannabinol and CBD concentration-dependently induced the expression of CYP1A1 mRNA, whereas cannabinol showed little or no induction. Among the phytocannabinoids tested, CBD was the most potent inducer of CYP1A1 expression. The induction of CYP1A1 expression by CBD was significantly attenuated by the knockdown of AhR expression with AhR small interfering RNAs. The role of protein tyrosine kinases (PTKs) in the CBD-mediated induction of CYP1A1 was then examined using herbimycin A, a PTK inhibitor. The upregulation of CYP1A1 by CBD was significantly suppressed by herbimycin A as was the induction by omeprazole but not 3-methylcholanthrene. The inducibility of CYP1A1 by CBD-related compounds was examined to clarify the structural requirements for CBD-mediated CYP1A1 induction. Olivetol, which corresponds to the pentylresorcinol moiety of CBD, significantly induced the expression of CYP1A1, whereas d-limonene, CBD-2\u0027-monomethyl ether, and CBD-2\u0027,6\u0027-dimethyl ether did not. \u003cbr/\u003eSignificance: These results showed that CBD may have induced human CYP1A1 expression through the activation of PTK-dependent AhR signaling, in which two phenolic hydroxyl groups in the pentylresorcinol moiety of CBD may play structurally important roles. (C) 2015 Elsevier Inc. All rights reserved."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yamaori, Satoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106326", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kinugasa, Yuka", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106327", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Jiang, Rongrong", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106328", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takeda, Shuso", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106329", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamamoto, Ikuo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106330", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe, Kazuhito", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106331", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-09-18"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Manuscript (LifeSci 2015 Yamaori).pdf", "filesize": [{"value": "454.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 454000.0, "url": {"label": "Manuscript (LifeSci 2015 Yamaori).pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/20107/files/Manuscript (LifeSci 2015 Yamaori).pdf"}, "version_id": "8ae9a8ff-1e58-4a2f-af33-278c33655b28"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Marijuana", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cannabidiol", "subitem_subject_scheme": "Other"}, {"subitem_subject": "CYP1A1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Induction", "subitem_subject_scheme": "Other"}, {"subitem_subject": "AhR", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Protein tyrosine kinase", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Cannabidiol induces expression of human cytochrome P450 1A1 that is possibly mediated through aryl hydrocarbon receptor signaling in HepG2 cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Cannabidiol induces expression of human cytochrome P450 1A1 that is possibly mediated through aryl hydrocarbon receptor signaling in HepG2 cells", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["883"], "permalink_uri": "http://hdl.handle.net/10091/00020868", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2018-09-18"}, "publish_date": "2018-09-18", "publish_status": "0", "recid": "20107", "relation": {}, "relation_version_is_last": true, "title": ["Cannabidiol induces expression of human cytochrome P450 1A1 that is possibly mediated through aryl hydrocarbon receptor signaling in HepG2 cells"], "weko_shared_id": -1}