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  1. 050 医学部, 大学院医学系研究科
  2. 0501 学術論文

Engineered Bone Marrow-Derived Cell Sheets Restore Structure and Function of Radiation-Injured Rat Urinary Bladders

http://hdl.handle.net/10091/00020980
http://hdl.handle.net/10091/00020980
6ecb0d81-6e68-4acf-9485-2f2f98497e30
名前 / ファイル ライセンス アクション
26462441_02.pdf 26462441_02.pdf (10.4 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2018-10-31
タイトル
言語 en
タイトル Engineered Bone Marrow-Derived Cell Sheets Restore Structure and Function of Radiation-Injured Rat Urinary Bladders
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Imamura, Tetsuya

× Imamura, Tetsuya

WEKO 106622

en Imamura, Tetsuya

Search repository
Ogawa, Teruyuki

× Ogawa, Teruyuki

WEKO 106623

en Ogawa, Teruyuki

Search repository
Minagawa, Tomonori

× Minagawa, Tomonori

WEKO 106624

en Minagawa, Tomonori

Search repository
Yokoyama, Hitoshi

× Yokoyama, Hitoshi

WEKO 106625

en Yokoyama, Hitoshi

Search repository
Nakazawa, Masaki

× Nakazawa, Masaki

WEKO 106626

en Nakazawa, Masaki

Search repository
Nishizawa, Osamu

× Nishizawa, Osamu

WEKO 106627

en Nishizawa, Osamu

Search repository
Ishizuka, Osamu

× Ishizuka, Osamu

WEKO 106628

en Ishizuka, Osamu

Search repository
信州大学研究者総覧へのリンク
氏名 Ishizuka, Osamu
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.gmfaHekh.html
出版者
出版者 MARY ANN LIEBERT, INC
引用
内容記述タイプ Other
内容記述 TISSUE ENGINEERING PART A.21(9-10):1600-1610(2015)
書誌情報 TISSUE ENGINEERING PART A

巻 21, 号 9-10, p. 1600-1610, 発行日 2015-03-26
抄録
内容記述タイプ Abstract
内容記述 Previously, we reported that implantation of isolated single bone marrow-derived cells into radiation-injured urinary bladders could restore structure and function. However, injections of isolated single cells had some limitations. Thus, in this study, we produced bone marrow-derived cell sheets in temperature-responsive culture dishes that release the monolayer sheets intact. We then determined whether the produced cell sheets could restore function to irradiated urinary bladders. Twenty female 10-week-old Sprague-Dawley (SD) rats were irradiated with 2 gray once a week for 5 weeks. Bone marrow cells harvested from two male 17-week-old green fluorescence protein-transfected SD rats were placed in primary culture for 7 days. Bone marrow cell-derived outgrowths were harvested by enzymatic digestion and transferred into the atelocollagen-coated temperature-responsive culture dishes for 2 days. To harvest the secondarily cultured cells as monolayer sheets, a support membrane was put in each culture dish, and then the temperature was reduced to 20°C. Each released cell sheet was then patched onto the irradiated anterior bladder wall (n=10). As controls, cell-free sheets were similarly patched (n=10). After 4 weeks, transplanted cells were detected on the bladder walls. The cell sheet-transplanted bladders had smooth muscle layers and acetylcholinesterase-positive nerve fibers in proportions that were significantly larger than those of the control bladders. In addition, the cell sheet-transplanted bladders had reduced prolyl 4-hydroxylase beta (P4HB)-positive regions of collagen synthesis and apoptosis within the smooth muscle layers. In cystometric investigations, threshold pressures, voiding interval, micturition volume, and bladder capacity in the cell sheet-transplantation group were significantly higher than those in the control group. Residual volume of the cell sheet-transplantation group was significantly lower compared with the control. There were 24 growth factor mRNAs in the cell sheet-transplanted urinary bladders that were expressed greater than or equal to two-fold over the controls. In conclusion, cell sheet engineering has great potential to restore damaged urinary bladders.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ PISSN
収録物識別子 1937-3341
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA12306003
PubMed
識別子タイプ PMID
関連識別子 https://www.ncbi.nlm.nih.gov/pubmed/25669695
関連名称 25669695
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1089/ten.tea.2014.0592
関連名称 10.1089/ten.tea.2014.0592
権利
権利情報 Final publication is available from Mary Ann Liebert, Inc., publishers https://doi.org/10.1089/ten.tea.2014.0592
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000353952300012
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