Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2018-10-31 |
タイトル |
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タイトル |
Engineered Bone Marrow-Derived Cell Sheets Restore Structure and Function of Radiation-Injured Rat Urinary Bladders |
言語 |
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言語 |
eng |
DOI |
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関連識別子 |
https://doi.org/10.1089/ten.tea.2014.0592 |
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関連名称 |
10.1089/ten.tea.2014.0592 |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Imamura, Tetsuya
Ogawa, Teruyuki
Minagawa, Tomonori
Yokoyama, Hitoshi
Nakazawa, Masaki
Nishizawa, Osamu
Ishizuka, Osamu
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信州大学研究者総覧へのリンク |
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氏名 |
Ishizuka, Osamu |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.gmfaHekh.html |
出版者 |
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出版者 |
MARY ANN LIEBERT, INC |
引用 |
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内容記述 |
TISSUE ENGINEERING PART A.21(9-10):1600-1610(2015) |
書誌情報 |
TISSUE ENGINEERING PART A
巻 21,
号 9-10,
p. 1600-1610,
発行日 2015-03-26
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抄録 |
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内容記述 |
Previously, we reported that implantation of isolated single bone marrow-derived cells into radiation-injured urinary bladders could restore structure and function. However, injections of isolated single cells had some limitations. Thus, in this study, we produced bone marrow-derived cell sheets in temperature-responsive culture dishes that release the monolayer sheets intact. We then determined whether the produced cell sheets could restore function to irradiated urinary bladders. Twenty female 10-week-old Sprague-Dawley (SD) rats were irradiated with 2 gray once a week for 5 weeks. Bone marrow cells harvested from two male 17-week-old green fluorescence protein-transfected SD rats were placed in primary culture for 7 days. Bone marrow cell-derived outgrowths were harvested by enzymatic digestion and transferred into the atelocollagen-coated temperature-responsive culture dishes for 2 days. To harvest the secondarily cultured cells as monolayer sheets, a support membrane was put in each culture dish, and then the temperature was reduced to 20°C. Each released cell sheet was then patched onto the irradiated anterior bladder wall (n=10). As controls, cell-free sheets were similarly patched (n=10). After 4 weeks, transplanted cells were detected on the bladder walls. The cell sheet-transplanted bladders had smooth muscle layers and acetylcholinesterase-positive nerve fibers in proportions that were significantly larger than those of the control bladders. In addition, the cell sheet-transplanted bladders had reduced prolyl 4-hydroxylase beta (P4HB)-positive regions of collagen synthesis and apoptosis within the smooth muscle layers. In cystometric investigations, threshold pressures, voiding interval, micturition volume, and bladder capacity in the cell sheet-transplantation group were significantly higher than those in the control group. Residual volume of the cell sheet-transplantation group was significantly lower compared with the control. There were 24 growth factor mRNAs in the cell sheet-transplanted urinary bladders that were expressed greater than or equal to two-fold over the controls. In conclusion, cell sheet engineering has great potential to restore damaged urinary bladders. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
1937-3341 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA12306003 |
PubMed |
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関連識別子 |
https://www.ncbi.nlm.nih.gov/pubmed/25669695 |
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関連名称 |
25669695 |
権利 |
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権利情報 |
Final publication is available from Mary Ann Liebert, Inc., publishers https://doi.org/10.1089/ten.tea.2014.0592 |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000353952300012 |