{"_buckets": {"deposit": "7c3bd645-a5aa-4346-bfa7-0ec9b3e009c9"}, "_deposit": {"id": "20246", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "20246"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00020246", "sets": ["462"]}, "author_link": ["106852", "106853", "106854", "106855", "106856", "106857", "106858", "106859", "106860", "106861", "106862", "106863", "106864"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-12-19", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "39383", "bibliographicVolumeNumber": "6", "bibliographic_titles": [{"bibliographic_title": "SCIENTIFIC REPORTS"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Structural cardiac remodeling, accompanying cytoskeletal reorganization of cardiac cells, is a major clinical outcome of diastolic heart failure. A highly local Ca2+ influx across the plasma membrane has been suggested to code signals to induce Rho GTPase-mediated fibrosis, but it is obscure how the heart specifically decodes the local Ca2+ influx as a cytoskeletal reorganizing signal under the conditions of the rhythmic Ca2+ handling required for pump function. We found that an inhibition of transient receptor potential canonical 3 (TRPC3) channel activity exhibited resistance to Rho-mediated maladaptive fibrosis in pressure-overloaded mouse hearts. Proteomic analysis revealed that microtubule-associated Rho guanine nucleotide exchange factor, GEF-H1, participates in TRPC3-mediated RhoA activation induced by mechanical stress in cardiomyocytes and transforming growth factor (TGF) β stimulation in cardiac fibroblasts. We previously revealed that TRPC3 functionally interacts with microtubule-associated NADPH oxidase (Nox) 2, and inhibition of Nox2 attenuated mechanical stretch-induced GEF-H1 activation in cardiomyocytes. Finally, pharmacological TRPC3 inhibition significantly suppressed fibrotic responses in human cardiomyocytes and cardiac fibroblasts. These results strongly suggest that microtubule-localized TRPC3-GEF-H1 axis mediates fibrotic responses commonly in cardiac myocytes and fibroblasts induced by physico-chemical stimulation", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "SCIENTIFIC REPORTS. 6: 39383(2016)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "Kuwahara, Koichiro", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.HVchWCcp.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000389889600001"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "NATURE PUBLISHING GROUP"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "27991560"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://www.ncbi.nlm.nih.gov/pubmed/27991560", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1038/srep39383"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1038/srep39383", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/"}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2045-2322", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2045-2322", "subitem_source_identifier_type": "PISSN"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "PLURIPOTENT STEM-CELLS; TRPC3 CHANNELS; HYPERTROPHY; HEART; ACTIVATION; STRESS; FIBROBLASTS; CONTRIBUTES; DYSFUNCTION; MODULATION"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Structural cardiac remodeling, accompanying cytoskeletal reorganization of cardiac cells, is a major clinical outcome of diastolic heart failure. A highly local Ca2+ influx across the plasma membrane has been suggested to code signals to induce Rho GTPase-mediated fibrosis, but it is obscure how the heart specifically decodes the local Ca2+ influx as a cytoskeletal reorganizing signal under the conditions of the rhythmic Ca2+ handling required for pump function. We found that an inhibition of transient receptor potential canonical 3 (TRPC3) channel activity exhibited resistance to Rho-mediated maladaptive fibrosis in pressure-overloaded mouse hearts. Proteomic analysis revealed that microtubule-associated Rho guanine nucleotide exchange factor, GEF-H1, participates in TRPC3-mediated RhoA activation induced by mechanical stress in cardiomyocytes and transforming growth factor (TGF)beta stimulation in cardiac fibroblasts. We previously revealed that TRPC3 functionally interacts with microtubuleassociated NADPH oxidase (Nox) 2, and inhibition of Nox2 attenuated mechanical stretch-induced GEF-H1 activation in cardiomyocytes. Finally, pharmacological TRPC3 inhibition significantly suppressed fibrotic responses in human cardiomyocytes and cardiac fibroblasts. These results strongly suggest that microtubule-localized TRPC3-GEF-H1 axis mediates fibrotic responses commonly in cardiac myocytes and fibroblasts induced by physico-chemical stimulation."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Numaga-Tomita, Takuro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106852", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitajima, Naoyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106853", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuroda, Takuya", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106854", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishimura, Akiyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106855", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Miyano, Kei", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106856", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yasuda, Satoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106857", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuwahara, Koichiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106858", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato, Yoji", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106859", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ide, Tomomi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106860", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Birnbaumer, Lutz", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106861", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sumimoto, Hideki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106862", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mori, Yasuo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106863", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishida, Motohiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106864", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-10-31"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "srep39383.pdf", "filesize": [{"value": "2.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/", "licensetype": "license_note", "mimetype": "application/pdf", "size": 2000000.0, "url": {"label": "srep39383.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/20246/files/srep39383.pdf"}, "version_id": "34645427-1b25-4afb-922e-001096b7ee60"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Heart failure", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ion channel signalling", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "TRPC3-GEF-H1 axis mediates pressure overload-induced cardiac fibrosis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "TRPC3-GEF-H1 axis mediates pressure overload-induced cardiac fibrosis", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["462"], "permalink_uri": "http://hdl.handle.net/10091/00021004", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2018-10-31"}, "publish_date": "2018-10-31", "publish_status": "0", "recid": "20246", "relation": {}, "relation_version_is_last": true, "title": ["TRPC3-GEF-H1 axis mediates pressure overload-induced cardiac fibrosis"], "weko_shared_id": -1}