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However, the biosynthesis of di‐C‐glucosyl flavonoids has not been elucidated at the molecular level. Here, we identified the C‐glycosyltransferases (CGTs) FcCGT (UGT708G1) and CuCGT (UGT708G2) as the primary enzymes involved in the biosynthesis of di‐C‐glucosyl flavonoids in the citrus plants kumquat (Fortunella crassifolia) and satsuma mandarin (Citrus unshiu), respectively. The amino acid sequences of these CGTs were 98% identical, indicating that CGT genes are highly conserved in the citrus family. The recombinant enzymes FcCGT and CuCGT utilized 2‐hydroxyflavanones, dihydrochalcone, and their mono‐C‐glucosides as sugar acceptors and produced corresponding di‐C‐glucosides. The Km and kcat values of FcCGT toward phloretin were \u003c0.5 μm and 12.0 sec−1, and those toward nothofagin (3ʹ‐C‐glucosylphloretin) were 14.4 μm and 5.3 sec−1, respectively; these values are comparable with those of other glycosyltransferases reported to date. Transcripts of both CGT genes were found to concentrate in various plant organs, and particularly in leaves. Our results suggest that di‐C‐glucosyl flavonoid biosynthesis proceeds via a single enzyme using either 2‐hydroxyflavanones or phloretin as a substrate in citrus plants. 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Transcripts of both CGT genes were found to concentrate in various plant organs, and particularly in leaves. Our results suggest that di-C-glucosyl flavonoid biosynthesis proceeds via a single enzyme using either 2-hydroxyflavanones or phloretin as a substrate in citrus plants. 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C‐Glycosyltransferases catalyzing the formation of di‐C‐glucosyl flavonoids in citrus plants
http://hdl.handle.net/10091/00021308
http://hdl.handle.net/10091/000213083c0897bb-4edb-4493-b2e0-02ee7773cee8
名前 / ファイル | ライセンス | アクション |
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26450120_01.pdf (9.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-03-18 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | C‐Glycosyltransferases catalyzing the formation of di‐C‐glucosyl flavonoids in citrus plants | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | di-C-glucosylation, flavonoid C-glycosyltransferase, Fortunella crassifolia, 24 Citrus unshiu, phloretin di-C-glucoside, vicenin-2 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Ito, Takamitsu
× Ito, Takamitsu× Fujimoto, Shunsuke× Suito, Fumiaki× Shimosaka, Makoto× Taguchi, Goro |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Shimosaka, Makoto | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Taguchi, Goro | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html | |||||
出版者 | ||||||
出版者 | WILEY | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Plant Journal.91(2):187-198(2017) | |||||
書誌情報 |
Plant Journal 巻 91, 号 2, p. 187-198, 発行日 2017-07-03 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Citrus plants accumulate many kinds of flavonoids, including di‐C‐glucosyl flavonoids, which have attracted considerable attention due to their health benefits. However, the biosynthesis of di‐C‐glucosyl flavonoids has not been elucidated at the molecular level. Here, we identified the C‐glycosyltransferases (CGTs) FcCGT (UGT708G1) and CuCGT (UGT708G2) as the primary enzymes involved in the biosynthesis of di‐C‐glucosyl flavonoids in the citrus plants kumquat (Fortunella crassifolia) and satsuma mandarin (Citrus unshiu), respectively. The amino acid sequences of these CGTs were 98% identical, indicating that CGT genes are highly conserved in the citrus family. The recombinant enzymes FcCGT and CuCGT utilized 2‐hydroxyflavanones, dihydrochalcone, and their mono‐C‐glucosides as sugar acceptors and produced corresponding di‐C‐glucosides. The Km and kcat values of FcCGT toward phloretin were <0.5 μm and 12.0 sec−1, and those toward nothofagin (3ʹ‐C‐glucosylphloretin) were 14.4 μm and 5.3 sec−1, respectively; these values are comparable with those of other glycosyltransferases reported to date. Transcripts of both CGT genes were found to concentrate in various plant organs, and particularly in leaves. Our results suggest that di‐C‐glucosyl flavonoid biosynthesis proceeds via a single enzyme using either 2‐hydroxyflavanones or phloretin as a substrate in citrus plants. In addition, Escherichia coli cells expressing CGT genes were found to be capable of producing di‐C‐glucosyl flavonoids, which is promising for commercial production of these valuable compounds. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0960-7412 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10846815 | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://www.ncbi.nlm.nih.gov/pubmed/28370711 | |||||
関連名称 | 28370711 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1111/tpj.13555 | |||||
関連名称 | 10.1111/tpj.13555 | |||||
権利 | ||||||
権利情報 | This is the peer reviewed version of the following article: [Plant Journal.91(2):187-198(2017)], which has been published in final form at [10.1111/tpj.13555]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000404795500001 |