{"_buckets": {"deposit": "c6f4cf9e-fd4b-4e7f-bb68-bd09a0a1d7a5"}, "_deposit": {"id": "20768", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "20768"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00020768", "sets": []}, "author_link": ["108104"], "item_14_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-02-13", "bibliographicIssueDateType": "Issued"}}]}, "item_14_date_granted_8": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2019-02-13"}]}, "item_14_degree_grantor_10": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "信州大学(Shinshu university)"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "13601", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_14_degree_name_9": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（医学）"}]}, "item_14_description_26": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "雑誌に発表。PEDIATRIC BLOOD \u0026 CANCER. 65(11):e27261 (2018); doi:10.1002/pbc.27261.", "subitem_description_type": "Other"}]}, "item_14_description_37": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Thesis", "subitem_description_type": "Other"}]}, "item_14_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "倉田　敬. Panobinostat inhibits the proliferation of CD34+CD38-cells under stimulation of hematopoietic growth factors on AGM-S3 cells in juvenile myelomonocytic leukemia（Panobinostatは若年性骨髄単球性白血病におけるCD34陽性CD38陰性細胞のAGM-S3上での造血因子依存性増殖を抑制する）. 信州大学, 2019, 博士論文.", "subitem_description_type": "Other"}]}, "item_14_dissertation_number_7": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "13601乙第1224号"}]}, "item_14_link_74": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000445194700001"}]}, "item_14_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "信州大学"}]}, "item_14_relation_54": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "30014555"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/30014555/", "subitem_relation_type_select": "PMID"}}]}, "item_14_relation_55": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1002/pbc.27261"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1002/pbc.27261", "subitem_relation_type_select": "DOI"}}]}, "item_14_rights_69": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This is the peer reviewed version of the following article: Pediatr. Blood Cancer 65(11): e27261, 2018, which has been published in final form at https://doi.org/10.1002/pbc.27261 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."}]}, "item_14_select_71": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_14_text_11": {"attribute_name": "学位の区分", "attribute_value_mlt": [{"subitem_text_value": "doctoral"}]}, "item_14_text_12": {"attribute_name": "学位の分野", "attribute_value_mlt": [{"subitem_text_value": "医学"}]}, "item_14_text_13": {"attribute_name": "学位の報告番号", "attribute_value_mlt": [{"subitem_text_value": "乙第1224号"}]}, "item_14_text_76": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "5-azacytidine; juvenile myelomonocytic leukemia; neoplastic stem cells; panobinostat"}]}, "item_14_text_77": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "DNA METHYLTRANSFERASE INHIBITORS; MYELODYSPLASTIC SYNDROME; ORAL PANOBINOSTAT; SOLID TUMORS; MUTATIONS; LBH589; AZACITIDINE; SETBP1; LINES"}]}, "item_14_textarea_75": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "BackgroundEncouraging responses to histone deacetylase inhibitors have been reported for hematologic malignancies. Here, we report effects of panobinostat and 5-azacytidine on the proliferation of juvenile myelomonocytic leukemia (JMML) CD34(+) cells. ProcedureWe previously reported that stimulation of JMML CD34(+) cells with stem cell factor and thrombopoietin on irradiated murine AGM-S3 cells led to substantial expansion of JMML CD34(+) cells that contained leukemic stem cells capable of transplantation into immunodeficient mice. Using this culture system, we evaluated effects of panobinostat and 5-azacytidine on the proliferation of JMML CD34(+) cells. ResultsPanobinostat dose dependently reduced the numbers of day 7 CD34(+) cells generated under stimulation of hematopoietic growth factors on AGM-S3 cells in all eight patients with JMML. These patients possessed various genetic and/or karyotypic abnormalities. CD34(+)CD38(-) cells were substantially more sensitive to panobinostat at 10 and 20nM than CD34(+)CD38(+) cells. Panobinostat, however, failed to influence the ability of AGM-S3 cells to stimulate JMML CD34(+) cell production. In contrast to HL60 cells, apoptosis and cell cycle arrest in panobinostat-mediated inhibition were at low levels in JMML. The inhibitor also suppressed the factor-dependent proliferation of normal CD34(+) cells on AGM-S3 cells. Meanwhile, no substantial inhibitory effects of 5-azacytidine on the growth of JMML CD34(+) cells were observed. ConclusionsThese results demonstrate that panobinostat directly suppresses the growth of JMML CD34(+) cells, in particular CD34(+)CD38(-) cells, regardless of the genetic abnormality type, suggesting that it is a useful antileukemic drug to target JMML stem cells at a pretransplant stage."}]}, "item_1627890986942": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_be7fb7dd8ff6fe43", "subitem_version_type": "NA"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "倉田, 敬", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "108104", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-06-14"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "18MR0008_yoshi.pdf", "filesize": [{"value": "129.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 129400.0, "url": {"label": "内容の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/20768/files/18MR0008_yoshi.pdf"}, "version_id": "18cd7921-cabd-4319-a237-c14b02bbcf4a"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-06-14"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "18MR0008_shinsa.pdf", "filesize": [{"value": "88.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 88300.0, "url": {"label": "審査結果の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/20768/files/18MR0008_shinsa.pdf"}, "version_id": "8d2506f9-f09a-40c4-b7b1-338dc3ba6eee"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-16"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "18MR0008_ronbun.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "This is the peer reviewed version of the following article: Pediatr. Blood Cancer 65(11): e27261, 2018, which has been published in final form at https://doi.org/10.1002/pbc.27261 . 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