{"_buckets": {"deposit": "c686144d-6074-4512-875e-245f8c06efa8"}, "_deposit": {"id": "20770", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "20770"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00020770", "sets": []}, "author_link": ["108106"], "item_14_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-03-13", "bibliographicIssueDateType": "Issued"}}]}, "item_14_date_granted_8": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2019-03-13"}]}, "item_14_degree_grantor_10": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "信州大学(Shinshu university)"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "13601", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_14_degree_name_9": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（医学）"}]}, "item_14_description_26": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "雑誌に発表。MOLECULAR PAIN. 14:(2018); doi:10.1177/1744806918819942.", "subitem_description_type": "Other"}]}, "item_14_description_37": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Thesis", "subitem_description_type": "Other"}]}, "item_14_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "山本　克己. Knockdown of TRPV2 channels in sensory neurons increases limb use and weight bearing but dose not affect spontaneous flinching behavior in a mouse model of bone cancer.（一次知覚神経のTransient Receptor Potential Vanilloid Subfamily 2チャネルのノックダウンは、マウス骨がんモデルにおける自発痛には影響せず、体動時痛のみを改善する）. 信州大学, 2019, 博士論文.", "subitem_description_type": "Other"}]}, "item_14_dissertation_number_7": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "13601乙第1226号"}]}, "item_14_link_74": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000454196200001"}]}, "item_14_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "信州大学"}]}, "item_14_relation_55": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1177/1744806918819942"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1177/1744806918819942", "subitem_relation_type_select": "DOI"}}]}, "item_14_rights_69": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© The Author(s) 2018 / This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). "}]}, "item_14_select_71": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_14_text_11": {"attribute_name": "学位の区分", "attribute_value_mlt": [{"subitem_text_value": "doctoral"}]}, "item_14_text_12": {"attribute_name": "学位の分野", "attribute_value_mlt": [{"subitem_text_value": "医学"}]}, "item_14_text_13": {"attribute_name": "学位の報告番号", "attribute_value_mlt": [{"subitem_text_value": "乙第1226号"}]}, "item_14_text_76": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "transient receptor potential vanilloid subfamily 2; bone cancer pain; movement-related pain; small interference RNA"}]}, "item_14_text_77": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "CAPSAICIN-RECEPTOR HOMOLOG; SYSTEMIC MORPHINE; HIGH-THRESHOLD; PAIN; EXPRESSION; NOCICEPTORS; MECHANISMS"}]}, "item_14_textarea_75": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Bone cancer pain is a complex pain state involving ongoing pain and movement-related pain, which are thought to be caused by different mechanisms. Transient receptor potential vanilloid subfamily 1 (TRPV1) is involved in ongoing pain but not movement-related pain. The purpose of this study was to investigate the role of transient receptor potential vanilloid subfamily 2 (TRPV2) in bone cancer pain. Proportions of TRPV1- and TRPV2-immunoreactive neurons in lumbar dorsal root ganglia innervating the femurs of male mice were examined by using Fluoro-Gold. Mice were intrathecally injected with small interfering RNA (siRNA) against TRPV2 or scrambled siRNA for three consecutive days from day 14 after sarcoma injection into the left femur. In the mice with bone cancer, the number of spontaneous flinches was quantified for assessment of ongoing pain, and limb use and weight bearing were assessed as indications of movement-related pain. Changes in TRPV2 protein levels in dorsal root ganglion were evaluated by Western blotting. We also examined the effects of intrathecal administration of siRNA against TRPV2 or scrambled siRNA on thermal and mechanical sensitivities in normal mice without tumors. The proportions of TRPV1-immunoreactive and TRPV2-immunoreactive neurons were 21% and 22% of neurons in dorsal root ganglia innervating the femur, respectively. Tumor-bearing mice exhibited an increased number of spontaneous flinches and impaired limb use and weight bearing at day 13 after sarcoma injection. TRPV2 protein level in dorsal root ganglia at day 13 was comparable to that at baseline. siRNA against TRPV2 significantly improved limb use and weight bearing but did not affect the number of spontaneous flinches compared to those in the group treated with scrambled siRNA. siRNA against TRPV2 did not affect thermal or mechanical sensitivity in normal mice. The results suggest that TRPV2 is involved in movement-related pain but not ongoing pain in mice with bone cancer."}]}, "item_1627890986942": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_be7fb7dd8ff6fe43", "subitem_version_type": "NA"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "山本, 克己", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "108106", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-06-13"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "18MR0010_yoshi.pdf", "filesize": [{"value": "78.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 78700.0, "url": {"label": "内容の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/20770/files/18MR0010_yoshi.pdf"}, "version_id": "e2a9f110-2b1b-40ee-92ba-072c1de5cc5c"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-06-13"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "18MR0010_shinsa.pdf", "filesize": [{"value": "67.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 67500.0, "url": {"label": "審査結果の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/20770/files/18MR0010_shinsa.pdf"}, "version_id": "0f99b472-79b5-4a6e-b150-249e2482db7e"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-06-13"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "18MR0010_ronbun.pdf", "filesize": [{"value": "828.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_9", "mimetype": "application/pdf", "size": 828600.0, "url": {"label": "博士論文の全文", "objectType": "fulltext", "url": "https://soar-ir.repo.nii.ac.jp/record/20770/files/18MR0010_ronbun.pdf"}, "version_id": "02e8ae91-c1e4-49b4-8244-6b41166fc2e3"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Knockdown of TRPV2 channels in sensory neurons increases limb use and weight bearing but dose not affect spontaneous flinching behavior in a mouse model of bone cancer.（一次知覚神経のTransient Receptor Potential Vanilloid Subfamily 2チャネルのノックダウンは、マウス骨がんモデルにおける自発痛には影響せず、体動時痛のみを改善する）", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Knockdown of TRPV2 channels in sensory neurons increases limb use and weight bearing but dose not affect spontaneous flinching behavior in a mouse model of bone cancer.（一次知覚神経のTransient Receptor Potential Vanilloid Subfamily 2チャネルのノックダウンは、マウス骨がんモデルにおける自発痛には影響せず、体動時痛のみを改善する）", "subitem_title_language": "en"}]}, "item_type_id": "14", "owner": "1", "path": ["849"], "permalink_uri": "http://hdl.handle.net/10091/00021528", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2019-06-14"}, "publish_date": "2019-06-14", "publish_status": "0", "recid": "20770", "relation": {}, "relation_version_is_last": true, "title": ["Knockdown of TRPV2 channels in sensory neurons increases limb use and weight bearing but dose not affect spontaneous flinching behavior in a mouse model of bone cancer.（一次知覚神経のTransient Receptor Potential Vanilloid Subfamily 2チャネルのノックダウンは、マウス骨がんモデルにおける自発痛には影響せず、体動時痛のみを改善する）"], "weko_shared_id": -1}