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  1. 050 医学部, 大学院医学系研究科
  2. 0501 学術論文

Biofabricated Structures Reconstruct Functional Urinary Bladders in Radiation-Injured Rat Bladders

http://hdl.handle.net/10091/00021610
008340d3-2d0a-4074-bfa2-bb2b35ea50d7
名前 / ファイル ライセンス アクション
15K10622_01.pdf 15K10622_01.pdf (8.1 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2019-09-09
タイトル
言語 en
タイトル Biofabricated Structures Reconstruct Functional Urinary Bladders in Radiation-Injured Rat Bladders
言語
言語 eng
キーワード
主題Scheme Other
主題 biofabrication
キーワード
主題Scheme Other
主題 bone marrow-derived cell
キーワード
主題Scheme Other
主題 urinary bladder
キーワード
主題Scheme Other
主題 radiation injury
キーワード
主題Scheme Other
主題 lower urinary tract symptoms
キーワード
主題Scheme Other
主題 rat
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Imamura, Tetsuya

× Imamura, Tetsuya

WEKO 108398

en Imamura, Tetsuya

Search repository
Shimamura, Mitsuru

× Shimamura, Mitsuru

WEKO 108399

en Shimamura, Mitsuru

Search repository
Ogawa, Teruyuki

× Ogawa, Teruyuki

WEKO 108400

en Ogawa, Teruyuki

Search repository
Minagawa, Tomonori

× Minagawa, Tomonori

WEKO 108401

en Minagawa, Tomonori

Search repository
Nagai, Takashi

× Nagai, Takashi

WEKO 108402

en Nagai, Takashi

Search repository
Gautam, Sudha Silwal

× Gautam, Sudha Silwal

WEKO 108403

en Gautam, Sudha Silwal

Search repository
Ishizuka, Osamu

× Ishizuka, Osamu

WEKO 108404

en Ishizuka, Osamu

Search repository
信州大学研究者総覧へのリンク
氏名 Imamura, Tetsuya
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.ypACOmyC.html
信州大学研究者総覧へのリンク
氏名 Ogawa, Teruyuki
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.yhTUgpkh.html
信州大学研究者総覧へのリンク
氏名 Minagawa, Tomonori
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.jCfagUkh.html
信州大学研究者総覧へのリンク
氏名 Ishizuka, Osamu
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.gmfaHekh.html
出版者
出版者 MARY ANN LIEBERT, INC
引用
内容記述タイプ Other
内容記述 TISSUE ENGINEERING PART A.24(21-22):1574-1587(2018)
書誌情報 TISSUE ENGINEERING PART A

巻 24, 号 21-22, p. 1574-1587, 発行日 2018-11-01
抄録
内容記述タイプ Abstract
内容記述 The ability to repair damaged urinary bladders through the application of bone marrow-derived cells is in the earliest stages of development. We investigated the application of bone marrow-derived cells to repair radiation-injured bladders. We used a three-dimensional bioprinting robot system to biofabricate bone marrow-derived cell structures. We then determined if the biofabricated structures could restore the tissues and functions of radiation-injured bladders. The bladders of female 10-week-old Sprague-Dawley (SD) rats were irradiated with 2-Gy once a week for 5 weeks. Adherent and proliferating bone marrow-derived cells harvested from the femurs of male 17-week-old green fluorescence protein-transfected Tg-SD rats were cultured in collagen-coated flasks. Bone marrow-derived cell spheroids were formed in 96-well plates. Three layers of spheroids were assembled by the bioprinter onto a 9x9 microneedle array. The assembled spheroids were perfusion cultured for 7 days, and then the microneedle array was removed. Two weeks after the last radiation treatment, the biofabricated structures were transplanted into an incision on the anterior wall of the bladders (n=10). Control rats received the same surgery but without the biofabricated structures (sham-structure, n=12). At 2 and 4 weeks after surgery, the sham-structure control bladder tissues exhibited disorganized smooth muscle layers, decreased nerve cells, and significant fibrosis with increased presence of fibrosis-marker P4HB-positive cells and hypoxia-marker hypoxia-induced factor 1 (HIF1)-positive cells. The transplanted structures survived within the recipient tissues, and blood vessels extended within them from the recipient tissues. The bone marrow-derived cells in the structures differentiated into smooth muscle cells and formed smooth muscle clusters. The recipient tissues near the transplanted structures had distinct smooth muscle layers and reconstructed nerve cells, and only minimal fibrosis with decreased presence of P4HB- and HIF1-positive cells. At 4 weeks after surgery, the sham-structure control rats exhibited significant urinary frequency symptoms with irregular and short voiding intervals, and low micturition volumes. In contrast, the structure-transplanted rats had regular micturition with longer voiding intervals and higher micturition volumes compared with the control rats. Furthermore, the residual volume of the structure-transplanted rats was lower than for the controls. Therefore, transplantation of biofabricated bone marrow-derived cell structures reconstructed functional bladders.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ PISSN
収録物識別子 1937-3341
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA12306003
PubMed
関連識別子
識別子タイプ PMID
関連識別子 https://www.ncbi.nlm.nih.gov/pubmed/29737234
関連名称
関連名称 29737234
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1089/ten.tea.2017.0533
関連名称
関連名称 10.1089/ten.tea.2017.0533
権利
権利情報 Final publication is available from Mary Ann Liebert, Inc., publishers https://doi.org/10.1089/ten.tea.2017.0533
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000449368500002
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