| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-09-09 |
| タイトル |
|
|
タイトル |
Biofabricated Structures Reconstruct Functional Urinary Bladders in Radiation-Injured Rat Bladders |
| 言語 |
|
|
言語 |
eng |
| DOI |
|
|
|
関連識別子 |
https://doi.org/10.1089/ten.tea.2017.0533 |
|
|
関連名称 |
10.1089/ten.tea.2017.0533 |
| キーワード |
|
|
主題 |
biofabrication, bone marrow-derived cell, urinary bladder, radiation injury, lower urinary tract symptoms, rat |
| 資源タイプ |
|
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資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Imamura, Tetsuya
Shimamura, Mitsuru
Ogawa, Teruyuki
Minagawa, Tomonori
Nagai, Takashi
Gautam, Sudha Silwal
Ishizuka, Osamu
|
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Imamura, Tetsuya |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.ypACOmyC.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Ogawa, Teruyuki |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.yhTUgpkh.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Minagawa, Tomonori |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.jCfagUkh.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Ishizuka, Osamu |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.gmfaHekh.html |
| 出版者 |
|
|
出版者 |
MARY ANN LIEBERT, INC |
| 引用 |
|
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内容記述 |
TISSUE ENGINEERING PART A.24(21-22):1574-1587(2018) |
| 書誌情報 |
TISSUE ENGINEERING PART A
巻 24,
号 21-22,
p. 1574-1587,
発行日 2018-11-01
|
| 抄録 |
|
|
内容記述 |
The ability to repair damaged urinary bladders through the application of bone marrow-derived cells is in the earliest stages of development. We investigated the application of bone marrow-derived cells to repair radiation-injured bladders. We used a three-dimensional bioprinting robot system to biofabricate bone marrow-derived cell structures. We then determined if the biofabricated structures could restore the tissues and functions of radiation-injured bladders. The bladders of female 10-week-old Sprague-Dawley (SD) rats were irradiated with 2-Gy once a week for 5 weeks. Adherent and proliferating bone marrow-derived cells harvested from the femurs of male 17-week-old green fluorescence protein-transfected Tg-SD rats were cultured in collagen-coated flasks. Bone marrow-derived cell spheroids were formed in 96-well plates. Three layers of spheroids were assembled by the bioprinter onto a 9x9 microneedle array. The assembled spheroids were perfusion cultured for 7 days, and then the microneedle array was removed. Two weeks after the last radiation treatment, the biofabricated structures were transplanted into an incision on the anterior wall of the bladders (n=10). Control rats received the same surgery but without the biofabricated structures (sham-structure, n=12). At 2 and 4 weeks after surgery, the sham-structure control bladder tissues exhibited disorganized smooth muscle layers, decreased nerve cells, and significant fibrosis with increased presence of fibrosis-marker P4HB-positive cells and hypoxia-marker hypoxia-induced factor 1 (HIF1)-positive cells. The transplanted structures survived within the recipient tissues, and blood vessels extended within them from the recipient tissues. The bone marrow-derived cells in the structures differentiated into smooth muscle cells and formed smooth muscle clusters. The recipient tissues near the transplanted structures had distinct smooth muscle layers and reconstructed nerve cells, and only minimal fibrosis with decreased presence of P4HB- and HIF1-positive cells. At 4 weeks after surgery, the sham-structure control rats exhibited significant urinary frequency symptoms with irregular and short voiding intervals, and low micturition volumes. In contrast, the structure-transplanted rats had regular micturition with longer voiding intervals and higher micturition volumes compared with the control rats. Furthermore, the residual volume of the structure-transplanted rats was lower than for the controls. Therefore, transplantation of biofabricated bone marrow-derived cell structures reconstructed functional bladders. |
| 資源タイプ（コンテンツの種類） |
|
| ISSN |
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収録物識別子タイプ |
PISSN |
|
収録物識別子 |
1937-3341 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12306003 |
| PubMed |
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|
識別子タイプ |
PMID |
|
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関連識別子 |
https://www.ncbi.nlm.nih.gov/pubmed/29737234 |
|
|
関連名称 |
29737234 |
| 権利 |
|
|
権利情報 |
Final publication is available from Mary Ann Liebert, Inc., publishers https://doi.org/10.1089/ten.tea.2017.0533 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| WoS |
|
|
URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000449368500002 |
15K10622_01.pdf (8.1 MB)
