| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-09-09 |
| タイトル |
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|
タイトル |
Theobromine suppresses adipogenesis through enhancement of CCAAT-enhancer-binding protein beta degradation by adenosine receptor A1 |
| 言語 |
|
|
言語 |
eng |
| DOI |
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|
|
関連識別子 |
https://doi.org/10.1016/j.bbamcr.2017.09.017 |
|
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関連名称 |
10.1016/j.bbamcr.2017.09.017 |
| キーワード |
|
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主題 |
Adenosine receptor, Adipogenesis, CCAAT/enhancer-binding protein, Sumoylation, Theobromine |
| 資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Mitani, Takakazu
Watanabe, Shun
Yoshioka, Yasukiyo
Katayama, Shigeru
Nakamura, Soichiro
Ashida, Hitoshi
|
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Mitani, Takakazu |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.ypcCPUkh.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Katayama, Shigeru |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.HFkaPUkh.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Nakamura, Soichiro |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.OpyhuNDp.html |
| 出版者 |
|
|
出版者 |
ELSEVIER SCIENCE BV |
| 引用 |
|
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内容記述 |
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH.1864(12):2438-2448(2017) |
| 書誌情報 |
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
巻 1864,
号 12,
p. 2438-2448,
発行日 2017-12
|
| 抄録 |
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内容記述 |
Theobromine, a methylxanthine derived from cacao beans, reportedly has various health-promoting properties but molecular mechanism by which effects of theobromine on adipocyte differentiation and adipogenesis remains unclear. In this study, we aimed to clarify the molecular mechanisms of the anti-adipogenic effect of theobromine in vitro and in vivo. ICR mice (4 week-old) were administered with theobromine (0.1 g/kg) for 7 days. Theobromine administration attenuated gains in body and epididymal adipose tissue weights in mice and suppressed expression of adipogenic-associated genes in mouse adipose tissue. In 3T3-L1 preadipocytes, theobromine caused degradation of C/EBP beta protein by the ubiquitin-proteasome pathway. Pull down assay showed that theobromine selectively interacts with adenosine receptor A1(AR1), and AR1 knockdown inhibited theobromine-induced C/ESPfi degradation. Theobromine increased sumoylation of C/EBP beta' at Lys133. Expression of the small ubiquitin-like modifier (SUMO)-specific protease 2 (SENP2) gene, coding for a desumoylation enzyme, was suppressed by theobromine. In vivo knockdown studies showed that AR1 knockdown in mice attenuated the anti-adipogenic effects of theobromine in younger mice. Theobromine suppresses adipocyte differentiation and induced C/EBPP degradation by increasing its sumoylation. Furthermore, the inhibition of AR1 signaling is important for theobromine-induced C/EBP beta degradation. |
| 資源タイプ（コンテンツの種類） |
|
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0167-4889 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA1050609X |
| PubMed |
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/28965824/ |
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関連名称 |
28965824 |
| 権利 |
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|
権利情報 |
© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 出版タイプ |
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|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| WoS |
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|
URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000416195800019 |
16K18701_02.pdf (1.7 MB)
