WEKO3
アイテム
{"_buckets": {"deposit": "93dd1ca8-aac4-4350-98b7-a4bf15a09684"}, "_deposit": {"id": "20858", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "20858"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00020858", "sets": ["1017"]}, "author_link": ["108439", "108440", "108441", "108442", "108443", "108444"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "12", "bibliographicPageEnd": "2448", "bibliographicPageStart": "2438", "bibliographicVolumeNumber": "1864", "bibliographic_titles": [{"bibliographic_title": "BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Theobromine, a methylxanthine derived from cacao beans, reportedly has various health-promoting properties but molecular mechanism by which effects of theobromine on adipocyte differentiation and adipogenesis remains unclear. In this study, we aimed to clarify the molecular mechanisms of the anti-adipogenic effect of theobromine in vitro and in vivo. ICR mice (4 week-old) were administered with theobromine (0.1 g/kg) for 7 days. Theobromine administration attenuated gains in body and epididymal adipose tissue weights in mice and suppressed expression of adipogenic-associated genes in mouse adipose tissue. In 3T3-L1 preadipocytes, theobromine caused degradation of C/EBP beta protein by the ubiquitin-proteasome pathway. Pull down assay showed that theobromine selectively interacts with adenosine receptor A1(AR1), and AR1 knockdown inhibited theobromine-induced C/ESPfi degradation. Theobromine increased sumoylation of C/EBP beta\u0027 at Lys133. Expression of the small ubiquitin-like modifier (SUMO)-specific protease 2 (SENP2) gene, coding for a desumoylation enzyme, was suppressed by theobromine. In vivo knockdown studies showed that AR1 knockdown in mice attenuated the anti-adipogenic effects of theobromine in younger mice. Theobromine suppresses adipocyte differentiation and induced C/EBPP degradation by increasing its sumoylation. Furthermore, the inhibition of AR1 signaling is important for theobromine-induced C/EBP beta degradation.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ(コンテンツの種類)", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH.1864(12):2438-2448(2017)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "Mitani, Takakazu", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.ypcCPUkh.html"}, {"subitem_link_text": "Katayama, Shigeru", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.HFkaPUkh.html"}, {"subitem_link_text": "Nakamura, Soichiro", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.OpyhuNDp.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000416195800019"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "ELSEVIER SCIENCE BV"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "28965824"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/28965824/", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/j.bbamcr.2017.09.017"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.bbamcr.2017.09.017", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ "}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0167-4889", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0167-4889", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA1050609X", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "MECHANISMS LINKING OBESITY; ADIPOCYTE DIFFERENTIATION; PPAR-GAMMA; C/EBP-BETA; INSULIN-RESISTANCE; A(2A) RECEPTORS; SKELETAL-MUSCLE; ADIPOSE-TISSUE; 3T3-L1 CELLS; KINASE-A"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "Theobromine, a methylxanthine derived from cacao beans, reportedly has various health-promoting properties but molecular mechanism by which effects of theobromine on adipocyte differentiation and adipogenesis remains unclear. In this study, we aimed to clarify the molecular mechanisms of the anti-adipogenic effect of theobromine in vitro and in vivo. ICR mice (4 week-old) were administered with theobromine (0.1 g/kg) for 7 days. Theobromine administration attenuated gains in body and epididymal adipose tissue weights in mice and suppressed expression of adipogenic-associated genes in mouse adipose tissue. In 3T3-L1 preadipocytes, theobromine caused degradation of C/EBP beta protein by the ubiquitin-proteasome pathway. Pull down assay showed that theobromine selectively interacts with adenosine receptor A1(AR1), and AR1 knockdown inhibited theobromine-induced C/ESPfi degradation. Theobromine increased sumoylation of C/EBP beta\u0027 at Lys133. Expression of the small ubiquitin-like modifier (SUMO)-specific protease 2 (SENP2) gene, coding for a desumoylation enzyme, was suppressed by theobromine. In vivo knockdown studies showed that AR1 knockdown in mice attenuated the anti-adipogenic effects of theobromine in younger mice. Theobromine suppresses adipocyte differentiation and induced C/EBPP degradation by increasing its sumoylation. Furthermore, the inhibition of AR1 signaling is important for theobromine-induced C/EBP beta degradation."}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Adenosine receptor; Adipogenesis; CCAAT/enhancer-binding protein; Sumoylation; Theobromine"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mitani, Takakazu"}], "nameIdentifiers": [{"nameIdentifier": "108439", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe, Shun"}], "nameIdentifiers": [{"nameIdentifier": "108440", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshioka, Yasukiyo"}], "nameIdentifiers": [{"nameIdentifier": "108441", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Katayama, Shigeru"}], "nameIdentifiers": [{"nameIdentifier": "108442", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakamura, Soichiro"}], "nameIdentifiers": [{"nameIdentifier": "108443", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ashida, Hitoshi"}], "nameIdentifiers": [{"nameIdentifier": "108444", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-09-09"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "16K18701_02.pdf", "filesize": [{"value": "1.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/", "licensetype": "license_note", "mimetype": "application/pdf", "size": 1700000.0, "url": {"label": "16K18701_02.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/20858/files/16K18701_02.pdf"}, "version_id": "34d54adc-fdf4-4f95-adee-fcdf62764127"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Adenosine receptor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Adipogenesis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "CCAAT/enhancer-binding protein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Sumoylation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Theobromine", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Theobromine suppresses adipogenesis through enhancement of CCAAT-enhancer-binding protein beta degradation by adenosine receptor A1", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Theobromine suppresses adipogenesis through enhancement of CCAAT-enhancer-binding protein beta degradation by adenosine receptor A1", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1017"], "permalink_uri": "http://hdl.handle.net/10091/00021615", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2019-09-09"}, "publish_date": "2019-09-09", "publish_status": "0", "recid": "20858", "relation": {}, "relation_version_is_last": true, "title": ["Theobromine suppresses adipogenesis through enhancement of CCAAT-enhancer-binding protein beta degradation by adenosine receptor A1"], "weko_shared_id": -1}
Theobromine suppresses adipogenesis through enhancement of CCAAT-enhancer-binding protein beta degradation by adenosine receptor A1
http://hdl.handle.net/10091/00021615
http://hdl.handle.net/10091/00021615aec5af48-f972-4419-9fe2-aedd77c830e9
名前 / ファイル | ライセンス | アクション |
---|---|---|
16K18701_02.pdf (1.7 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2019-09-09 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Theobromine suppresses adipogenesis through enhancement of CCAAT-enhancer-binding protein beta degradation by adenosine receptor A1 | |||||
言語 | ||||||
言語 | eng | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.bbamcr.2017.09.017 | |||||
関連名称 | 10.1016/j.bbamcr.2017.09.017 | |||||
キーワード | ||||||
主題 | Adenosine receptor, Adipogenesis, CCAAT/enhancer-binding protein, Sumoylation, Theobromine | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Mitani, Takakazu
× Mitani, Takakazu× Watanabe, Shun× Yoshioka, Yasukiyo× Katayama, Shigeru× Nakamura, Soichiro× Ashida, Hitoshi |
|||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Mitani, Takakazu | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ypcCPUkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Katayama, Shigeru | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HFkaPUkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Nakamura, Soichiro | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OpyhuNDp.html | |||||
出版者 | ||||||
出版者 | ELSEVIER SCIENCE BV | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH.1864(12):2438-2448(2017) | |||||
書誌情報 |
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 巻 1864, 号 12, p. 2438-2448, 発行日 2017-12 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Theobromine, a methylxanthine derived from cacao beans, reportedly has various health-promoting properties but molecular mechanism by which effects of theobromine on adipocyte differentiation and adipogenesis remains unclear. In this study, we aimed to clarify the molecular mechanisms of the anti-adipogenic effect of theobromine in vitro and in vivo. ICR mice (4 week-old) were administered with theobromine (0.1 g/kg) for 7 days. Theobromine administration attenuated gains in body and epididymal adipose tissue weights in mice and suppressed expression of adipogenic-associated genes in mouse adipose tissue. In 3T3-L1 preadipocytes, theobromine caused degradation of C/EBP beta protein by the ubiquitin-proteasome pathway. Pull down assay showed that theobromine selectively interacts with adenosine receptor A1(AR1), and AR1 knockdown inhibited theobromine-induced C/ESPfi degradation. Theobromine increased sumoylation of C/EBP beta' at Lys133. Expression of the small ubiquitin-like modifier (SUMO)-specific protease 2 (SENP2) gene, coding for a desumoylation enzyme, was suppressed by theobromine. In vivo knockdown studies showed that AR1 knockdown in mice attenuated the anti-adipogenic effects of theobromine in younger mice. Theobromine suppresses adipocyte differentiation and induced C/EBPP degradation by increasing its sumoylation. Furthermore, the inhibition of AR1 signaling is important for theobromine-induced C/EBP beta degradation. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0167-4889 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1050609X | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://pubmed.ncbi.nlm.nih.gov/28965824/ | |||||
関連名称 | 28965824 | |||||
権利 | ||||||
権利情報 | © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000416195800019 |