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Distinct cell proliferation, myogenic differentiation, and gene expression in skeletal muscle myoblasts of layer and broiler chickens
http://hdl.handle.net/10091/00021765
http://hdl.handle.net/10091/00021765b1107581-12a0-4852-bb49-4e7ab8a80b24
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
s41598-019-52946-4.pdf (4.7 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2019-11-12 | |||||
| タイトル | ||||||
| タイトル | Distinct cell proliferation, myogenic differentiation, and gene expression in skeletal muscle myoblasts of layer and broiler chickens | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1038/s41598-019-52946-4 | |||||
| 関連名称 | 10.1038/s41598-019-52946-4 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Nihashi, Yuma
× Nihashi, Yuma× Umezawa, Koji× Shinji, Sayaka× Hamaguchi, Yu× Kobayashi, Hisato× Kono, Tomohiro× Ono, Tamao× Kagami, Hiroshi× Takaya, Tomohide |
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| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Umezawa, Koji | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.yVcFPhkh.html | |||||
| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Ono, Tamao | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html | |||||
| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Kagami, Hiroshi | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html | |||||
| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Takaya, Tomohide | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.yUcePUkh.html | |||||
| 出版者 | ||||||
| 出版者 | NATURE PUBLISHING GROUP | |||||
| 書誌情報 |
SCIENTIFIC REPORTS 巻 9, p. 16527, 発行日 2019-11-11 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Myoblasts play a central role during skeletal muscle formation and growth. Precise understanding of myoblast properties is thus indispensable for meat production. Herein, we report the cellular characteristics and gene expression profiles of primary-cultured myoblasts of layer and broiler chickens. Broiler myoblasts actively proliferated and promptly differentiated into myotubes compared to layer myoblasts, which corresponds well with the muscle phenotype of broilers. Transcriptomes of layer and broiler myoblasts during differentiation were quantified by RNA sequencing. Ontology analyses of the differentially expressed genes (DEGs) provided a series of extracellular proteins as putative markers for characterization of chicken myogenic cells. Another ontology analyses demonstrated that broiler myogenic cells are rich in cell cycle factors and muscle components. Independent of these semantic studies, principal component analysis (PCA) statistically defined two gene sets: one governing myogenic differentiation and the other segregating layers and broilers. Thirteen candidate genes were identified with a combined study of the DEGs and PCA that potentially contribute to proliferation or differentiation of chicken myoblasts. We experimentally proved that one of the candidates, enkephalin, an opioid peptide, suppresses myoblast growth. Our results present a new perspective that the opioids present in feeds may influence muscle development of domestic animals. | |||||
| 資源タイプ（コンテンツの種類） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2045-2322 | |||||
| PubMed | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/31712718/ | |||||
| 関連名称 | 31712718 | |||||
| 権利 | ||||||
| 権利情報 | © The Author(s) 2019 / This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| WoS | ||||||
| 表示名 | Web of Science | |||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000495613400010 | |||||
