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Chicken stem cell factor enhances primordial germ cell proliferation cooperatively with fibroblast growth factor 2
http://hdl.handle.net/10091/00021882
http://hdl.handle.net/10091/00021882479d31d4-b968-4abd-9ecd-e36a3b2c0aa1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
26450376_06.pdf (1.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-02-28 | |||||
| タイトル | ||||||
| タイトル | Chicken stem cell factor enhances primordial germ cell proliferation cooperatively with fibroblast growth factor 2 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1262/jrd.2015-128 | |||||
| 関連名称 | 10.1262/jrd.2015-128 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Chicken | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Germline chimera | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Primordial germ cells (PGCs) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Stem cell factor (SCF) | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
MIYAHARA, Daichi
× MIYAHARA, Daichi× OISHI, Isao× MAKINO, Ryuichi× KURUMISAWA, Nozomi× NAKAYA, Ryuma× ONO, Tamao× KAGAMI, Hiroshi× TAGAMI, Takahiro |
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| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | MIYAHARA, Daichi | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uhfmbCkF.html | |||||
| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | ONO, Tamao | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html | |||||
| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | KAGAMI, Hiroshi | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html | |||||
| 出版者 | ||||||
| 出版者 | THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal of Reproduction and Development.62(2):143-149(2016) | |||||
| 書誌情報 |
Journal of Reproduction and Development 巻 62, 号 2, p. 143-149, 発行日 2016 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | An in vitro culture system of chicken primordial germ cells (PGCs) has been recently developed, but the growth factor involved in the proliferation of PGCs is largely unknown. In the present study, we investigated the growth effects of chicken stem cell factor (chSCF) on the in vitro proliferation of chicken PGCs. We established two feeder cell lines (buffalo rat liver cells; BRL cells) that stably express the putative secreted form of chSCF (chSCF1-BRL) and membrane bound form of chSCF (chSCF2-BRL). Cultured PGC lines were incubated on chSCF1 or chSCF2-BRL feeder cells with fibroblast growth factor 2 (FGF2), and growth effects of each chSCF isoform were investigated. The in vitro proliferation rate of the PGCs cultured on chSCF2-BRL at 20 days of culture was more than threefold higher than those cultured on chSCF1-BRL cells and more than fivefold higher than those cultured on normal BRL cells. Thus, use of chSCF2-BRL feeder layer was effective for in vitro proliferation of chicken PGCs. However, the acceleration of PGC proliferation on chSCF2-BRL was not observed without FGF2, suggesting that chSCF2 would act as a proliferation co-factor of FGF2. We transferred the PGCs cultured on chSCF2-BRL cells to recipient embryos, generated germline chimeric chickens and assessed the germline competency of cultured PGCs by progeny test. Donor-derived progenies were obtained, and the frequency of germline transmission was 3.39%. The results of this study demonstrate that chSCF2 induces hyperproliferation of chicken PGCs retaining germline competency in vitro in cooperation with FGF2. | |||||
| 資源タイプ（コンテンツの種類） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0916-8818 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10936678 | |||||
| PubMed | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/26727404/ | |||||
| 関連名称 | 26727404 | |||||
| 権利 | ||||||
| 権利情報 | This article is licensed under a Creative Commons [Attribution-NonCommercial-NoDerivatives 4.0 International] license. https://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
