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A Safe, Simple, and Facile Staining Method Using Polysiloxanes for High-Contrast Visualization of Gelator Aggregates by Transmission Electron Microscopy
http://hdl.handle.net/10091/00021970
http://hdl.handle.net/10091/00021970771b2a11-5fc0-4939-ad65-8895b660cee1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
15K05623_02.pdf (642.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-03-12 | |||||
| タイトル | ||||||
| タイトル | A Safe, Simple, and Facile Staining Method Using Polysiloxanes for High-Contrast Visualization of Gelator Aggregates by Transmission Electron Microscopy | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1246/bcsj.20180072 | |||||
| 関連名称 | 10.1246/bcsj.20180072 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Gelator aggregate | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Polysiloxane | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | TEM | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Hanabusa, Kenji
× Hanabusa, Kenji× Nakashima, Masashi× Funatsu, Eriko× Kishi, Sachiyo× Suzuki, Masahiro |
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| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Hanabusa, Kenji | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.WCyNjekV.html | |||||
| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Suzuki, Masahiro | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.WCyNjekV.html | |||||
| 出版者 | ||||||
| 出版者 | CHEMICAL SOC JAPAN | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN.91(7):1176-1185(2018) | |||||
| 書誌情報 |
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 巻 91, 号 7, p. 1176-1185, 発行日 2018-07 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The staining of TEM samples using Si atoms was investigated using aggregates of loose gels formed by twelve structurally different gelators in several solvents. Thirteen commercially available siloxanes were used as stains. TEM images of non-stained and OsO4-stained samples of molecular aggregates formed by the dodecamethylenediamide of N-methacryloyl-oxyethylaminocarbonyl-L-isoleucine in 1-propanol were poorly defined and low-contrast. However, the image of a methacryloyloxypropyl-terminated polydimethylsiloxane (S1)-stained sample was characterized by very clear bundles of fine fibers. The staining effect was explained by the wrapping of fibers, the stabilizing of the individual fibers, and reinforcing by S1. An S1 concentration of more than 5 mgmL(-1) was found to be necessary for satisfactory contrast. S1 was successfully applied to the observation of aggregates of eleven other gelators. S1 worked universally as an aggregate stain regardless of the gelator or solvent polarity. The staining effect was observed for other siloxanes. This effect was found to depend on the molecular weight of the siloxane (>1,000) rather than the kind of siloxane employed. Energy-dispersive X-ray spectroscopy indicated that the molecules of S1 gather on the surface of the fibers during drying, wrapping them. The results indicate that the present staining method guarantees reproducibility and universality. | |||||
| 資源タイプ（コンテンツの種類） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0009-2673 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00580132 | |||||
| 権利 | ||||||
| 権利情報 | © 2018 The Chemical Society of Japan. | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| WoS | ||||||
| 表示名 | Web of Science | |||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000437924100013 | |||||
