{"_buckets": {"deposit": "3f251e80-ca03-49c2-9b80-3e3de975339a"}, "_deposit": {"id": "21362", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "21362"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00021362", "sets": []}, "author_link": ["109453"], "item_14_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-03-31", "bibliographicIssueDateType": "Issued"}}]}, "item_14_date_granted_8": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2019-03-31"}]}, "item_14_degree_grantor_10": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "信州大学(Shinshu university)"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "13601", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_14_degree_name_9": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（保健学）"}]}, "item_14_description_26": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "雑誌に発表。ANNALS OF CLINICAL BIOCHEMISTRY. 56(5):574-582 (2019); doi:10.1177/0004563219849077.", "subitem_description_type": "Other"}]}, "item_14_description_37": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Thesis", "subitem_description_type": "Other"}]}, "item_14_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "堀　敦詞. Characterization of galactosyl and lactosyl sulfatide species in human serum by MALDI-TOF mass spectrometry （マトリックス支援レーザー脱離イオン化飛行時間型（MALDI-TOF）質量分析によるヒト血清中ガラクトシルスルファチド分子種とラクトシルスルファチド分子種の特徴付け）. 信州大学, 2019, 博士論文.", "subitem_description_type": "Other"}]}, "item_14_dissertation_number_7": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "13601甲第32号"}]}, "item_14_link_74": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000479257000007"}]}, "item_14_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "信州大学"}]}, "item_14_relation_54": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "31037952"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/31037952/", "subitem_relation_type_select": "PMID"}}]}, "item_14_relation_55": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1177/0004563219849077"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1177/0004563219849077", "subitem_relation_type_select": "DOI"}}]}, "item_14_rights_69": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Hori A, Yamaura M, Morita S, Uehara T, Honda T, Hidaka H, Characterization of galactosyl and lactosyl sulfatide species in human serum by MALDI-TOF mass spectrometry, Ann Clin Biochem. 56(5):574‐582. Copyright © The Author(s) 2019. DOI: 10.1177/0004563219849077."}]}, "item_14_select_71": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_14_text_11": {"attribute_name": "学位の区分", "attribute_value_mlt": [{"subitem_text_value": "doctoral"}]}, "item_14_text_12": {"attribute_name": "学位の分野", "attribute_value_mlt": [{"subitem_text_value": "保健学"}]}, "item_14_text_13": {"attribute_name": "学位の報告番号", "attribute_value_mlt": [{"subitem_text_value": "甲第32号"}]}, "item_14_text_76": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Mass spectrometry; sphingolipid; sulfatide; hydroxy fatty acid; serum lipids; sphingoid molecule; galactosyl sulfatide; lactosyl sulfatide; very long chain fatty acid; hydroxy fatty acid"}]}, "item_14_text_77": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "CELLS; GLYCOSPHINGOLIPIDS; CANCER; BRAIN"}]}, "item_14_textarea_75": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Background Sulfatides are found in a variety of tissues and serum lipoproteins. Sulfatide is a molecular species composed of various sphingoid bases, fatty acids and sugar chains; therefore, rapid analysis of the qualitative structure is important in clinical assessment. Methods In this study, sulfatide-rich fractions were isolated from serum lipids, and the sulfatide species were analysed by negative ion mode using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Results Sulfatide species identified in human serum included two different sugar chains, eight sphingoid molecules and various fatty acid side chains including hydroxy fatty acids. In total, 64 galactosyl sulfatides (SM4s) and 49 lactosyl sulfatides (SM3) were identified. Quantitatively, the amount of SM3 was less than 1% of the amount of SM4s. The fatty acids of SM4s of healthy serum (n = 8) were predominantly C16:0 and a hydroxylation C16:0 (C16:0h), followed by very long chain fatty acids (VLCFAs) predominant species, and SM3 was a major component of VLCFAs. Conclusion This present study described a simple method of human serum sulfatide analysis using MALDI-TOF MS. This method is suitable for clinical laboratories and is likely to increase the understanding of the roles of sulfatide species in both physiological and disease states."}]}, "item_1627890986942": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_be7fb7dd8ff6fe43", "subitem_version_type": "NA"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "堀, 敦詞", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "109453", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-06-03"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "16MK003A_yoshi.pdf", "filesize": [{"value": "184.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 184300.0, "url": {"label": "内容の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/21362/files/16MK003A_yoshi.pdf"}, "version_id": "6c299c49-a0d7-4173-9d1c-c50cb99aeb0a"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-06-03"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "16MK003A_shinsa.pdf", "filesize": [{"value": "141.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 141100.0, "url": {"label": "審査結果の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/21362/files/16MK003A_shinsa.pdf"}, "version_id": "926940e7-21d3-4699-9564-153962590a34"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-06-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "16MK003A_ronbun2.pdf", "filesize": [{"value": "774.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Hori A, Yamaura M, Morita S, Uehara T, Honda T, Hidaka H, Characterization of galactosyl and lactosyl sulfatide species in human serum by MALDI-TOF mass spectrometry, Ann Clin Biochem. 56(5):574‐582. 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