{"_buckets": {"deposit": "c230aad8-b57c-43a7-ab48-fa7f5a10b9cd"}, "_deposit": {"id": "21430", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "21430"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00021430", "sets": []}, "author_link": ["109557"], "item_14_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-02-27", "bibliographicIssueDateType": "Issued"}}]}, "item_14_date_granted_8": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2020-02-27"}]}, "item_14_degree_grantor_10": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "信州大学(Shinshu university)"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "13601", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_14_degree_name_9": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（医学）"}]}, "item_14_description_26": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "雑誌に発表。PLACENTA. 85:56-62 (2019); doi:10.1016/j.placenta.2019.06.377.", "subitem_description_type": "Other"}]}, "item_14_description_37": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Thesis", "subitem_description_type": "Other"}]}, "item_14_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "樋口　正太郎. Trophoblast type-specific expression of senescence markers in the human placenta （ヒト胎盤の栄養膜細胞のタイプ特異的な細胞老化マーカーの発現）. 信州大学, 2020, 博士論文.", "subitem_description_type": "Other"}]}, "item_14_dissertation_number_7": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "13601乙第1237号"}]}, "item_14_link_74": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000484393600008"}]}, "item_14_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "信州大学"}]}, "item_14_relation_54": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "31327484"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/31327484/", "subitem_relation_type_select": "PMID"}}]}, "item_14_relation_55": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/j.placenta.2019.06.377"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.placenta.2019.06.377", "subitem_relation_type_select": "DOI"}}]}, "item_14_rights_69": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2019 Elsevier Ltd."}]}, "item_14_select_71": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_14_text_11": {"attribute_name": "学位の区分", "attribute_value_mlt": [{"subitem_text_value": "doctoral"}]}, "item_14_text_12": {"attribute_name": "学位の分野", "attribute_value_mlt": [{"subitem_text_value": "医学"}]}, "item_14_text_13": {"attribute_name": "学位の報告番号", "attribute_value_mlt": [{"subitem_text_value": "乙第1237号"}]}, "item_14_text_76": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Cytotrophoblast; Syncytiotrophoblast; Cell senescence; Placenta; Senescence-associated beta-galactosidase"}]}, "item_14_text_77": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "CELLULAR SENESCENCE; TELOMERES; PREGNANCY; PATHWAYS; INVASION; PROTEIN; CELLS; CYCLE"}]}, "item_14_textarea_75": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Objectives: Cell senescence is irreversible cell cycle arrest. The human placenta is a unique organ that grows and matures during pregnancy until 40 weeks of gestation. However, the role of senescence in placental villi, particularly in the two types of trophoblast, has not yet been elucidated in detail. Therefore, we herein investigated the expression of cell senescence-related markers in trophoblast. Methods: Seventy normal placental tissues were used. The expression of senescence-associated beta-galactosidase (SA-beta-gal), cell senescence-related markers (p16, p21, and promyelocytic leukemia; PML), and a growth marker (MCM2) was immunohistochemically examined. The expression of these markers in BeWo cells before and after cell fusion using forskolin was also investigated. Results: The expression of MCM2 is detected in cytotrophoblast (CT). The expression of SA-beta-gal in CT is strong in the first and second trimesters, but weaker in the third trimester. Syncytiotrophoblast (ST) are negative in the first and second trimesters, but become positive in the third trimester. The immunohistochemical expression of p16, p21, and PML is stronger in CT than in ST throughout pregnancy. Furthermore, the expression of these markers in ST significantly increases as pregnancy advances. The expression of SA-beta-gal, PML, and p21 in BeWo cells is stronger after than before cell fusion. Conclusions: The proliferation and senescence of CT occurred in early to mid-pregnancy in association with syncytial fusion, while senescence was observed in ST in late pregnancy. This coordinated trophoblastic senescence may be essential for maintaining placental function."}]}, "item_1627890986942": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_be7fb7dd8ff6fe43", "subitem_version_type": "NA"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "樋口, 正太郎", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "109557", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-06-12"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "19MR0002_yoshi.pdf", "filesize": [{"value": "261.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 261399.99999999997, "url": {"label": "内容の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/21430/files/19MR0002_yoshi.pdf"}, "version_id": "9275f504-f910-4977-922e-153729e349ba"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-06-12"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "19MR0002_shinsa.pdf", "filesize": [{"value": "253.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 253000.0, "url": {"label": "審査結果の要旨", "url": "https://soar-ir.repo.nii.ac.jp/record/21430/files/19MR0002_shinsa.pdf"}, "version_id": "08261932-cfb5-423b-9b53-e950c4246b56"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-06-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "19MR0002_ronbun.pdf", "filesize": [{"value": "2.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "© 2019 Elsevier Ltd. All rights reserved.", "licensetype": "license_note", "mimetype": "application/pdf", "size": 2300000.0, "url": {"label": "博士論文の全文", "objectType": "fulltext", "url": "https://soar-ir.repo.nii.ac.jp/record/21430/files/19MR0002_ronbun.pdf"}, "version_id": "ee620594-a857-45bf-86e7-6a67314d1f33"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Trophoblast type-specific expression of senescence markers in the human placenta （ヒト胎盤の栄養膜細胞のタイプ特異的な細胞老化マーカーの発現）", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Trophoblast type-specific expression of senescence markers in the human placenta （ヒト胎盤の栄養膜細胞のタイプ特異的な細胞老化マーカーの発現）", "subitem_title_language": "en"}]}, "item_type_id": "14", "owner": "1", "path": ["849"], "permalink_uri": "http://hdl.handle.net/10091/00022187", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2020-12-18"}, "publish_date": "2020-12-18", "publish_status": "0", "recid": "21430", "relation": {}, "relation_version_is_last": true, "title": ["Trophoblast type-specific expression of senescence markers in the human placenta （ヒト胎盤の栄養膜細胞のタイプ特異的な細胞老化マーカーの発現）"], "weko_shared_id": -1}