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Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis
http://hdl.handle.net/10091/18521
http://hdl.handle.net/10091/185212d88d799-c841-4443-bbb0-17efb37d2d97
| 名前 / ファイル | ライセンス | アクション |
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Two‐dimensional_gel‐based_protein.pdf (244.3 kB)
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| Item type | 図書 / Book(1) | |||||||||||||
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| 公開日 | 2015-07-29 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| キーワード | ||||||||||||||
| 主題 | Protein standardization, Total protein density of a two-dimensional gel, Ratio of a protein against the total gel density, Quantitative western blot | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_2f33 | |||||||||||||
| タイプ | book | |||||||||||||
| 著者 |
Haniu, Hisao
× Haniu, Hisao
× Watanabe, Daisuke
× Kawashima, Yusuke
× Matsumoto, Hiroyuki
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| 信州大学研究者総覧へのリンク | ||||||||||||||
| 氏名 | Haniu, Hisao | |||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jakpPmSa.html | |||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | Springer New York | |||||||||||||
| 引用 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | Methods in Molecular Biology. Biji T, Kurien; R, Hal Scofield eds. 1312, Springer New York, 2015, p. 473-479. | |||||||||||||
| 書誌情報 |
Methods in Molecular Biology 巻 1312, p. 473-479, 発行日 2015 |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | In data presentation of biochemical investigation the amount of a target protein is shown in the y-axis against the x-axis representing time, concentrations of various agents, or other parameters. Western blot is a versatile and convenient tool in such an analysis to quantify and display the amount of proteins. In western blot, so-called housekeeping gene product(s), or "housekeeping proteins," are widely used as internal standards. The rationale of using housekeeping proteins for standardization of western blot is based on the assumption that the expression of chosen housekeeping gene is always constant, which could be false under certain physiological or pathological conditions. We have devised a two-dimensional gel-based standardization method in which the protein content of each sample is determined by scanning the total protein density of two-dimensional gels and the expression of each protein is quantified as the density ratio of each protein divided by the density of the total proteins on the two-dimensional gel. The advantage of this standardization method is that it is not based on any presumed "housekeeping proteins" that are supposed to be being expressed constantly under all physiological conditions. We will show that the total density of a two-dimensional gel can render a reliable protein standardization parameter by running western blot analysis on one of the proteins analyzed by two-dimensional gels. | |||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | Book chapter | |||||||||||||
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| 関連タイプ | isPartOf | |||||||||||||
| 識別子タイプ | ISSN | |||||||||||||
| 関連識別子 | 1064-3745 | |||||||||||||
| PubMed | ||||||||||||||
| 識別子タイプ | PMID | |||||||||||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/26044030/ | |||||||||||||
| 関連名称 | 26044030 | |||||||||||||
| DOI | ||||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1007/978-1-4939-2694-7_49 | |||||||||||||
| 関連名称 | 10.1007/978-1-4939-2694-7_49 | |||||||||||||
| 権利 | ||||||||||||||
| 権利情報 | The final publication is available at Springer via https://doi.org/10.1007/978-1-4939-2694-7_49 | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | AM | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
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Cite as
Haniu, Hisao, Watanabe, Daisuke, Kawashima, Yusuke, Matsumoto, Hiroyuki, 2015, Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis: Springer New York, p. 473–479.
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