ログイン
言語:

WEKO3

  • トップ
  • ランキング


インデックスリンク

インデックスツリー

  • RootNode

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

  1. 050 医学部, 大学院医学系研究科
  2. 0501 学術論文

Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis

http://hdl.handle.net/10091/18521
http://hdl.handle.net/10091/18521
2d88d799-c841-4443-bbb0-17efb37d2d97
名前 / ファイル ライセンス アクション
Two‐dimensional_gel‐based_protein.pdf Two‐dimensional_gel‐based_protein.pdf (244.3 kB)
Item type 図書 / Book(1)
公開日 2015-07-29
タイトル
タイトル Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis
言語 en
言語
言語 eng
キーワード
主題 Protein standardization, Total protein density of a two-dimensional gel, Ratio of a protein against the total gel density, Quantitative western blot
資源タイプ
資源 http://purl.org/coar/resource_type/c_2f33
タイプ book
著者 Haniu, Hisao

× Haniu, Hisao

en Haniu, Hisao

Search repository
Watanabe, Daisuke

× Watanabe, Daisuke

en Watanabe, Daisuke

Search repository
Kawashima, Yusuke

× Kawashima, Yusuke

en Kawashima, Yusuke

Search repository
Matsumoto, Hiroyuki

× Matsumoto, Hiroyuki

en Matsumoto, Hiroyuki

Search repository
信州大学研究者総覧へのリンク
氏名 Haniu, Hisao
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.jakpPmSa.html
出版者
出版者 Springer New York
引用
内容記述タイプ Other
内容記述 Methods in Molecular Biology. Biji T, Kurien; R, Hal Scofield eds. 1312, Springer New York, 2015, p. 473-479.
書誌情報 Methods in Molecular Biology

巻 1312, p. 473-479, 発行日 2015
抄録
内容記述タイプ Abstract
内容記述 In data presentation of biochemical investigation the amount of a target protein is shown in the y-axis against the x-axis representing time, concentrations of various agents, or other parameters. Western blot is a versatile and convenient tool in such an analysis to quantify and display the amount of proteins. In western blot, so-called housekeeping gene product(s), or "housekeeping proteins," are widely used as internal standards. The rationale of using housekeeping proteins for standardization of western blot is based on the assumption that the expression of chosen housekeeping gene is always constant, which could be false under certain physiological or pathological conditions. We have devised a two-dimensional gel-based standardization method in which the protein content of each sample is determined by scanning the total protein density of two-dimensional gels and the expression of each protein is quantified as the density ratio of each protein divided by the density of the total proteins on the two-dimensional gel. The advantage of this standardization method is that it is not based on any presumed "housekeeping proteins" that are supposed to be being expressed constantly under all physiological conditions. We will show that the total density of a two-dimensional gel can render a reliable protein standardization parameter by running western blot analysis on one of the proteins analyzed by two-dimensional gels.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Book chapter
ISSN
関連タイプ isPartOf
識別子タイプ ISSN
関連識別子 1064-3745
PubMed
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/26044030/
関連名称 26044030
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1007/978-1-4939-2694-7_49
関連名称 10.1007/978-1-4939-2694-7_49
権利
権利情報 The final publication is available at Springer via https://doi.org/10.1007/978-1-4939-2694-7_49
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 09:32:24.240188
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

Haniu, Hisao, Watanabe, Daisuke, Kawashima, Yusuke, Matsumoto, Hiroyuki, 2015, Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis: Springer New York, p. 473–479.

Loading...

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR 2.0
  • OAI-PMH JPCOAR 1.0
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3