| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2015-06-02 |
| タイトル |
|
|
タイトル |
Recombinant gamma T305A fibrinogen indicates severely impaired fibrin polymerization due to the aberrant function of hole 'a' and calcium binding sites |
| 言語 |
|
|
言語 |
eng |
| DOI |
|
|
|
関連識別子 |
https://doi.org/10.1016/j.thromres.2014.06.002 |
|
|
関連名称 |
10.1016/j.thromres.2014.06.002 |
| キーワード |
|
|
主題 |
hypodysfibrinogenemia, fibrin polymerization, high affinity calcium binding sites, hole 'a', D:D interaction sites |
| 資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Ikeda, Minami
Kobayashi, Tamaki
Arai, Shinpei
Mukai, Saki
Takezawa, Yuka
Terasawa, Fumiko
Okumura, Nobuo
|
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Okumura, Nobuo |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.OVnmgCkh.html |
| 出版者 |
|
|
出版者 |
PERGAMON-ELSEVIER SCIENCE LTD |
| 引用 |
|
|
内容記述 |
THROMBOSIS RESEARCH. 134(2):518-525 (2014) |
| 書誌情報 |
THROMBOSIS RESEARCH
巻 134,
号 2,
p. 518-525,
発行日 2014-08
|
| 抄録 |
|
|
内容記述 |
Introduction: We examined a 6-month-old girl with inherited fibrinogen abnormality and no history of bleeding or thrombosis. Routine coagulation screening tests showed a markedly low level of plasma fibrinogen determined by functional measurement and also a low level by antigenic measurement (functional/antigenic ratio = 0.295), suggesting hypodysfibrinogenemia. Materials and methods: DNA sequence analysis was performed, and gamma T305A fibrinogen was synthesized in Chinese hamster ovary cells based on the results. We then functionally analyzed and compared with that of nearby recombinant gamma N308K fibrinogen. Results: DNA sequence analysis revealed a heterozygous gamma T305A substitution (mature protein residue number). The gamma T305A fibrinogen indicated markedly impaired thrombin-catalyzed fibrin polymerization both in the presence or absence of 1 mM calcium ion compared with that of gamma N308K fibrinogen. Protection of plasmin degradation in the presence of calcium ion or Gly-Pro-Arg-Pro peptide (analogue for so-called knob 'A') and factor XIIIa-catalyzed fibrinogen crosslinking demonstrated that the calcium binding sites, hole 'a' and D:D interaction sites were all markedly impaired, whereas gamma N308K was impaired at the latter two sites. Molecular modeling demonstrated that gamma T305 is localized at a shorter distance than gamma N308 from the high affinity calcium binding site and hole 'a'. Conclusion: Our findings suggest that gamma T305 might be important for construction of the overall structure of the. module of fibrinogen. Substitution of gamma T305A leads to both dysfibrinogenemic and hypofibrinogenemic characterization, namely hypodysfibrinogenemia. We have already reported that recombinant gamma T305A fibrinogen was synthesized normally and secreted slightly, but was significantly reduced. |
| 資源タイプ（コンテンツの種類） |
|
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0049-3848 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00863148 |
| PubMed |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/24968960 |
|
|
関連名称 |
24968960 |
| 権利 |
|
|
権利情報 |
© 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| WoS |
|
|
URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000341309200049 |
Recombinant_gamma_T305A.pdf (1.1 MB)
