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Bone Marrow-Derived Cells Implanted into Radiation-Injured Urinary Bladders Reconstruct Functional Bladder Tissues in Rats
http://hdl.handle.net/10091/17583
a9c9d4a5-7068-49a4-84ad-6da3d59746ff
名前 / ファイル | ライセンス | アクション | |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-06-10 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Bone Marrow-Derived Cells Implanted into Radiation-Injured Urinary Bladders Reconstruct Functional Bladder Tissues in Rats | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Imamura, Tetsuya
× Imamura, Tetsuya× Ishizuka, Osamu× Lei, Zhang× Hida, Shigeaki× Sudha, Gautam Silwal× Kato, Haruaki× Nishizawa, Osamu |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Imamura, Tetsuya | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ypACOmyC.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Ishizuka, Osamu | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.gmfaHekh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Nishizawa, Osamu | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.WeyeWmyU.html | |||||
出版者 | ||||||
出版者 | MARY ANN LIEBERT INC | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | TISSUE ENGINEERING PART A. 18(15-16):1698-1709 (2012) | |||||
書誌情報 |
TISSUE ENGINEERING PART A 巻 18, 号 15-16, p. 1698-1709, 発行日 2012-08 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The purpose of this study was to determine whether bone marrow-derived cells implanted into radiation-injured urinary bladders could reconstruct functional bladder tissues. The pelvic region of anesthetized female Sprague-Dawley (SD) rats was irradiated with 2 Gy once a week for 5 weeks. After the last irradiation, the rats were maintained for 2 weeks. Bone marrow cells were harvested from the femurs of donor male green fluorescence protein (GFP)-transfected SD rats and cultured for 7 days. Two weeks after the last radiation exposure, the cultured adherent, proliferating bone marrow-derived cells were implanted into the walls of irradiated urinary bladders. For controls, cell-free solutions were similarly injected. Four weeks after donor cell or control implantations, cystometric, histological, and immunohistochemical investigations were performed. Two weeks after the last irradiation, the smooth muscle layers and nerve fibers of the irradiated urinary bladders were disorganized. The proportions of smooth muscle layer and nerve fiber areas were significantly decreased compared with sham-irradiated urinary bladders. In addition, the remaining smooth muscle cells within the irradiated urinary bladders expressed P4HB, an indicator of collagen synthesis. In the cystometric investigations, the voiding interval of irradiated rats was irregularly prolonged, 7.92 +/- 1.09 min, and the residual volume, 0.13 +/- 0.03 mL, was significantly higher compared with the sham-irradiated rats (5.50 +/- 0.43mL and 0.05 +/- 0.01 mL). After 4 weeks, the smooth muscle layers and nerve fibers in the cell-free control urinary bladders remained similar to the pre-implanted irradiated urinary bladders; however, the cell-implanted urinary bladders contained reconstructed smooth muscle layers and nerve fibers, the proportions of each were significantly higher than those in the cell-free injected controls. The expression of P4HB within the cell-implanted urinary bladders decreased. Some GFP-positive implanted cells differentiated into smooth muscle-and nerve-like cells and became organized into the reconstructed tissues. The voiding interval of the cell-implanted rats, 5.46 +/- 0.33 min, was regular and similar to that of the sham-irradiated rats, and significantly less than that of the cell-free injected controls, 7.39 +/- 0.54 min. The residual volume, 0.04 +/- 0.01 mL, was similar to that of the sham-irradiated rats and significantly decreased compared with that of the cell-free injected controls, 0.15 +/- 0.05 mL. Therefore, the implantation of bone marrow-derived cells is a potentially useful treatment for radiotherapy-induced urinary dysfunctions. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 1937-3341 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12306003 | |||||
PubMed | ||||||
関連識別子 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://pubmed.ncbi.nlm.nih.gov/22480258 | |||||
関連名称 | ||||||
関連名称 | 22480258 | |||||
DOI | ||||||
関連識別子 | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1089/ten.tea.2012.0061 | |||||
関連名称 | ||||||
関連名称 | 10.1089/ten.tea.2012.0061 | |||||
権利 | ||||||
権利情報 | This is a copy of an article published in the Tissue Engineering Part A. Copyright© 2012 Mary Ann Liebert, Inc.; Tissue Engineering Part A is available online at: http://online.liebertpub.com. | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000307999100016 |