WEKO3
AND
アイテム
{"_buckets": {"deposit": "e60c794f-3213-4c11-9f14-a66f723887da"}, "_deposit": {"id": "3780", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "3780"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00003780", "sets": ["461:462"]}, "author_link": ["6991", "6992", "6993", "6994", "6995", "6996", "6997", "6998", "6999", "7000", "7001", "7002", "7003"], "item_1628147817048": {"attribute_name": "\u51fa\u7248\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "1685", "bibliographicPageStart": "1679", "bibliographicVolumeNumber": "27", "bibliographic_titles": [{"bibliographic_title": "Toxicology in Vitro"}]}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "We examined the cytotoxicity of multi-walled carbon nanotubes (MWCNTs) and the resulting cytokine secretion in BEAS-2B cells or normal human bronchial epithelial cells (HBEpCs) in two types of culture media (Ham\u2019s F12 containing 10% FBS [Ham\u2019s F12] and serum-free growth medium [SFGM]). Cellular uptake of MWCNT was observed by fluorescent microscopy and analyzed using flow cytometry. Moreover, we evaluated whether MWCNT uptake was suppressed by 2 types of endocytosis inhibitors. We found that BEAS-2B cells cultured in Ham\u2019s F12 and HBEpCs cultured in SFGM showed similar biological responses, but BEAS-2B cells cultured in SFGM did not internalize MWCNTs, and the 50% inhibitory concentration value, i.e., the cytotoxicity, was increased by more than 10-fold. MWCNT uptake was suppressed by a clathrin-mediated endocytosis inhibitor and a caveolae-mediated endocytosis inhibitor in BEAS-2B cells cultured in Ham\u2019s F12 and HBEpCs cultured in SFGM. In conclusion, we suggest that BEAS-2B cells cultured in a medium containing serum should be used for the safety evaluation of nanomaterials as a model of normal human bronchial epithelial cells. However, the culture medium composition may affect the proteins that are expressed on the cytoplasmic membrane, which may influence the biological response to MWCNTs.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "Toxicology in Vitro. 27(6):1679-1685 (2013)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Haniu, Hisao", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.jakpPmSa.html"}, {"subitem_link_text": "Saito, Naoto", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.WpkhuUkh.html"}, {"subitem_link_text": "Aoki, Kaoru", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.WeDpbhym.html"}, {"subitem_link_text": "Kato, Hiroyuki", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.WVkCbaSp.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000324847800010"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "23648666"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/23648666", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/j.tiv.2013.04.012"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.tiv.2013.04.012", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "Copyright\u00a9 2013 The Authors. Published by Elsevier Ltd. / This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0887-2333", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0887-2333", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10678594", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Multi-walled carbon nanotube; BEAS-2B cells; Normal human bronchial epithelial cells; Cytotoxicity; Endocytosis"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "BRONCHIAL EPITHELIAL-CELLS; CELLULAR UPTAKE; IN-VITRO; NANOMATERIALS; TRANSFORMATION; NANOPARTICLES; CYTOTOXICITY; EXPRESSION; TOXICITY; VIRUS"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "We examined the cytotoxicity of multi-walled carbon nanotubes (MWCNTs) and the resulting cytokine secretion in BEAS-2B cells or normal human bronchial epithelial cells (HBEpCs) in two types of culture media (Ham\u0027s F12 containing 10% FBS [Ham\u0027s F12] and serum-free growth medium [SFGM]). Cellular uptake of MWCNT was observed by fluorescent microscopy and analyzed using flow cytometry. Moreover, we evaluated whether MWCNT uptake was suppressed by 2 types of endocytosis inhibitors. We found that BEAS-2B cells cultured in Ham\u0027s F12 and HBEpCs cultured in SFGM showed similar biological responses, but BEAS-2B cells cultured in SFGM did not internalize MWCNTs, and the 50% inhibitory concentration value, i.e., the cytotoxicity, was increased by more than 10-fold. MWCNT uptake was suppressed by a clathrin-mediated endocytosis inhibitor and a caveolae-mediated endocytosis inhibitor in BEAS-2B cells cultured in Ham\u0027s F12 and HBEpCs cultured in SFGM. In conclusion, we suggest that BEAS-2B cells cultured in a medium containing serum should be used for the safety evaluation of nanomaterials as a model of normal human bronchial epithelial cells. However, the culture medium composition may affect the proteins that are expressed on the cytoplasmic membrane, which may influence the biological response to MWCNTs. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Haniu, Hisao", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6991", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saito, Naoto", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6992", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsuda, Yoshikazu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6993", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsukahara, Tamotsu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6994", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Maruyama, Kayo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6995", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Usui, Yuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6996", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Aoki, Kaoru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6997", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takanashi, Seiji", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6998", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kobayashi, Shinsuke", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6999", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nomura, Hiroki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7000", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okamoto, Masanori", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7001", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimizu, Masayuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7002", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato, Hiroyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7003", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Culture_medium_type_affects_endocytosis_multi-walled_carbon_nanotubes.pdf", "filesize": [{"value": "1.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1300000.0, "url": {"label": "Culture_medium_type_affects_endocytosis_multi-walled_carbon_nanotubes.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/3780/files/Culture_medium_type_affects_endocytosis_multi-walled_carbon_nanotubes.pdf"}, "version_id": "6a1a2455-eacf-4178-93ad-308ac89d3ec7"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Multi-walled carbon nanotube", "subitem_subject_scheme": "Other"}, {"subitem_subject": "BEAS-2B cells", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Normal human bronchial epithelial cells", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cytotoxicity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Endocytosis", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Culture medium type affects endocytosis of multi-walled carbon nanotubes in BEAS-2B cells and subsequent biological response", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Culture medium type affects endocytosis of multi-walled carbon nanotubes in BEAS-2B cells and subsequent biological response", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["461/462"], "permalink_uri": "http://hdl.handle.net/10091/17038", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2013-05-31"}, "publish_date": "2013-05-31", "publish_status": "0", "recid": "3780", "relation": {}, "relation_version_is_last": true, "title": ["Culture medium type affects endocytosis of multi-walled carbon nanotubes in BEAS-2B cells and subsequent biological response"], "weko_shared_id": -1}
Culture medium type affects endocytosis of multi-walled carbon nanotubes in BEAS-2B cells and subsequent biological response
http://hdl.handle.net/10091/17038
0cd1d982-9cb5-4d12-b7d9-2850ff9295bf
名前 / ファイル | ライセンス | アクション | |
---|---|---|---|
![]() |
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2013-05-31 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Culture medium type affects endocytosis of multi-walled carbon nanotubes in BEAS-2B cells and subsequent biological response | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Multi-walled carbon nanotube | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | BEAS-2B cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Normal human bronchial epithelial cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cytotoxicity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Endocytosis | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Haniu, Hisao
× Haniu, Hisao× Saito, Naoto× Matsuda, Yoshikazu× Tsukahara, Tamotsu× Maruyama, Kayo× Usui, Yuki× Aoki, Kaoru× Takanashi, Seiji× Kobayashi, Shinsuke× Nomura, Hiroki× Okamoto, Masanori× Shimizu, Masayuki× Kato, Hiroyuki |
|||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Haniu, Hisao | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jakpPmSa.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Saito, Naoto | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.WpkhuUkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Aoki, Kaoru | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.WeDpbhym.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Kato, Hiroyuki | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.WVkCbaSp.html | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Toxicology in Vitro. 27(6):1679-1685 (2013) | |||||
書誌情報 |
Toxicology in Vitro 巻 27, 号 6, p. 1679-1685, 発行日 2013-09 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We examined the cytotoxicity of multi-walled carbon nanotubes (MWCNTs) and the resulting cytokine secretion in BEAS-2B cells or normal human bronchial epithelial cells (HBEpCs) in two types of culture media (Ham’s F12 containing 10% FBS [Ham’s F12] and serum-free growth medium [SFGM]). Cellular uptake of MWCNT was observed by fluorescent microscopy and analyzed using flow cytometry. Moreover, we evaluated whether MWCNT uptake was suppressed by 2 types of endocytosis inhibitors. We found that BEAS-2B cells cultured in Ham’s F12 and HBEpCs cultured in SFGM showed similar biological responses, but BEAS-2B cells cultured in SFGM did not internalize MWCNTs, and the 50% inhibitory concentration value, i.e., the cytotoxicity, was increased by more than 10-fold. MWCNT uptake was suppressed by a clathrin-mediated endocytosis inhibitor and a caveolae-mediated endocytosis inhibitor in BEAS-2B cells cultured in Ham’s F12 and HBEpCs cultured in SFGM. In conclusion, we suggest that BEAS-2B cells cultured in a medium containing serum should be used for the safety evaluation of nanomaterials as a model of normal human bronchial epithelial cells. However, the culture medium composition may affect the proteins that are expressed on the cytoplasmic membrane, which may influence the biological response to MWCNTs. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0887-2333 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10678594 | |||||
PubMed | ||||||
関連識別子 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://pubmed.ncbi.nlm.nih.gov/23648666 | |||||
関連名称 | ||||||
関連名称 | 23648666 | |||||
DOI | ||||||
関連識別子 | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.tiv.2013.04.012 | |||||
関連名称 | ||||||
関連名称 | 10.1016/j.tiv.2013.04.012 | |||||
権利 | ||||||
権利情報 | Copyright© 2013 The Authors. Published by Elsevier Ltd. / This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000324847800010 |