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  1. 050 医学部, 大学院医学系研究科
  2. 0501 学術論文

Culture medium type affects endocytosis of multi-walled carbon nanotubes in BEAS-2B cells and subsequent biological response

http://hdl.handle.net/10091/17038
http://hdl.handle.net/10091/17038
0cd1d982-9cb5-4d12-b7d9-2850ff9295bf
名前 / ファイル ライセンス アクション
Culture_medium_type_affects_endocytosis_multi-walled_carbon_nanotubes.pdf Culture_medium_type_affects_endocytosis_multi-walled_carbon_nanotubes.pdf (1.3 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2013-05-31
タイトル
タイトル Culture medium type affects endocytosis of multi-walled carbon nanotubes in BEAS-2B cells and subsequent biological response
言語
言語 eng
DOI
関連識別子 https://doi.org/10.1016/j.tiv.2013.04.012
関連名称 10.1016/j.tiv.2013.04.012
キーワード
主題 Multi-walled carbon nanotube, BEAS-2B cells, Normal human bronchial epithelial cells, Cytotoxicity, Endocytosis
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Haniu, Hisao

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Saito, Naoto

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Matsuda, Yoshikazu

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en Matsuda, Yoshikazu

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Tsukahara, Tamotsu

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Maruyama, Kayo

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Usui, Yuki

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en Usui, Yuki

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Aoki, Kaoru

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Takanashi, Seiji

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Kobayashi, Shinsuke

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Nomura, Hiroki

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Okamoto, Masanori

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Shimizu, Masayuki

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Kato, Hiroyuki

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信州大学研究者総覧へのリンク
氏名 Haniu, Hisao
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.jakpPmSa.html
信州大学研究者総覧へのリンク
氏名 Saito, Naoto
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.WpkhuUkh.html
信州大学研究者総覧へのリンク
氏名 Aoki, Kaoru
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.WeDpbhym.html
信州大学研究者総覧へのリンク
氏名 Kato, Hiroyuki
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.WVkCbaSp.html
出版者
出版者 Elsevier
引用
内容記述 Toxicology in Vitro. 27(6):1679-1685 (2013)
書誌情報 Toxicology in Vitro

巻 27, 号 6, p. 1679-1685, 発行日 2013-09
抄録
内容記述 We examined the cytotoxicity of multi-walled carbon nanotubes (MWCNTs) and the resulting cytokine secretion in BEAS-2B cells or normal human bronchial epithelial cells (HBEpCs) in two types of culture media (Ham’s F12 containing 10% FBS [Ham’s F12] and serum-free growth medium [SFGM]). Cellular uptake of MWCNT was observed by fluorescent microscopy and analyzed using flow cytometry. Moreover, we evaluated whether MWCNT uptake was suppressed by 2 types of endocytosis inhibitors. We found that BEAS-2B cells cultured in Ham’s F12 and HBEpCs cultured in SFGM showed similar biological responses, but BEAS-2B cells cultured in SFGM did not internalize MWCNTs, and the 50% inhibitory concentration value, i.e., the cytotoxicity, was increased by more than 10-fold. MWCNT uptake was suppressed by a clathrin-mediated endocytosis inhibitor and a caveolae-mediated endocytosis inhibitor in BEAS-2B cells cultured in Ham’s F12 and HBEpCs cultured in SFGM. In conclusion, we suggest that BEAS-2B cells cultured in a medium containing serum should be used for the safety evaluation of nanomaterials as a model of normal human bronchial epithelial cells. However, the culture medium composition may affect the proteins that are expressed on the cytoplasmic membrane, which may influence the biological response to MWCNTs.
資源タイプ(コンテンツの種類)
ISSN
収録物識別子タイプ PISSN
収録物識別子 0887-2333
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10678594
PubMed
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/23648666
関連名称 23648666
権利
権利情報 Copyright© 2013 The Authors. Published by Elsevier Ltd. / This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
WoS
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000324847800010
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Cite as

Haniu, Hisao, Saito, Naoto, Matsuda, Yoshikazu, Tsukahara, Tamotsu, Maruyama, Kayo, Usui, Yuki, Aoki, Kaoru, Takanashi, Seiji, Kobayashi, Shinsuke, Nomura, Hiroki, Okamoto, Masanori, Shimizu, Masayuki, Kato, Hiroyuki, 2013, Culture medium type affects endocytosis of multi-walled carbon nanotubes in BEAS-2B cells and subsequent biological response: Elsevier, 1679–1685 p.

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