| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-05-31 |
| タイトル |
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|
タイトル |
Culture medium type affects endocytosis of multi-walled carbon nanotubes in BEAS-2B cells and subsequent biological response |
| 言語 |
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言語 |
eng |
| DOI |
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関連識別子 |
https://doi.org/10.1016/j.tiv.2013.04.012 |
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関連名称 |
10.1016/j.tiv.2013.04.012 |
| キーワード |
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主題 |
Multi-walled carbon nanotube, BEAS-2B cells, Normal human bronchial epithelial cells, Cytotoxicity, Endocytosis |
| 資源タイプ |
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|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Haniu, Hisao
Saito, Naoto
Matsuda, Yoshikazu
Tsukahara, Tamotsu
Maruyama, Kayo
Usui, Yuki
Aoki, Kaoru
Takanashi, Seiji
Kobayashi, Shinsuke
Nomura, Hiroki
Okamoto, Masanori
Shimizu, Masayuki
Kato, Hiroyuki
|
| 信州大学研究者総覧へのリンク |
|
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氏名 |
Haniu, Hisao |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.jakpPmSa.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Saito, Naoto |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.WpkhuUkh.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Aoki, Kaoru |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.WeDpbhym.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Kato, Hiroyuki |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.WVkCbaSp.html |
| 出版者 |
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出版者 |
Elsevier |
| 引用 |
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内容記述 |
Toxicology in Vitro. 27(6):1679-1685 (2013) |
| 書誌情報 |
Toxicology in Vitro
巻 27,
号 6,
p. 1679-1685,
発行日 2013-09
|
| 抄録 |
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内容記述 |
We examined the cytotoxicity of multi-walled carbon nanotubes (MWCNTs) and the resulting cytokine secretion in BEAS-2B cells or normal human bronchial epithelial cells (HBEpCs) in two types of culture media (Ham’s F12 containing 10% FBS [Ham’s F12] and serum-free growth medium [SFGM]). Cellular uptake of MWCNT was observed by fluorescent microscopy and analyzed using flow cytometry. Moreover, we evaluated whether MWCNT uptake was suppressed by 2 types of endocytosis inhibitors. We found that BEAS-2B cells cultured in Ham’s F12 and HBEpCs cultured in SFGM showed similar biological responses, but BEAS-2B cells cultured in SFGM did not internalize MWCNTs, and the 50% inhibitory concentration value, i.e., the cytotoxicity, was increased by more than 10-fold. MWCNT uptake was suppressed by a clathrin-mediated endocytosis inhibitor and a caveolae-mediated endocytosis inhibitor in BEAS-2B cells cultured in Ham’s F12 and HBEpCs cultured in SFGM. In conclusion, we suggest that BEAS-2B cells cultured in a medium containing serum should be used for the safety evaluation of nanomaterials as a model of normal human bronchial epithelial cells. However, the culture medium composition may affect the proteins that are expressed on the cytoplasmic membrane, which may influence the biological response to MWCNTs. |
| 資源タイプ（コンテンツの種類） |
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| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0887-2333 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA10678594 |
| PubMed |
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識別子タイプ |
PMID |
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関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/23648666 |
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関連名称 |
23648666 |
| 権利 |
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権利情報 |
Copyright© 2013 The Authors. Published by Elsevier Ltd. / This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| WoS |
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|
URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000324847800010 |
Culture_medium_type_affects_endocytosis_multi-walled_carbon_nanotubes.pdf (1.3 MB)
