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The proximal C-terminus of alpha(1C) subunits is necessary for junctional membrane targeting of cardiac L-type calcium channels
http://hdl.handle.net/10091/16907
http://hdl.handle.net/10091/169075ca60f9e-5550-4e95-9bc6-ee3fee1999cd
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Proximal_C-terminus_α1C_subunits_necessary_junctional_membrane-targeting.pdf (1.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||||
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| 公開日 | 2013-04-01 | |||||||||||||||||||||||
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| タイトル | The proximal C-terminus of alpha(1C) subunits is necessary for junctional membrane targeting of cardiac L-type calcium channels | |||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||
| DOI | ||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1042/BJ20120773 | |||||||||||||||||||||||
| 関連名称 | 10.1042/BJ20120773 | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | cardiac myocyte, Ca-v 1.2, couplon, excitation-contraction coupling, junctional membrane complex | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| タイプ | journal article | |||||||||||||||||||||||
| 著者 |
Nakada, Tsutomu
× Nakada, Tsutomu
× Flucher, Bernhard E.
× Kashihara, Toshihide
× Sheng, Xiaona
× Shibazaki, Toshihide
× Horiuchi-Hirose, Miwa
× Gomi, Simmon
× Hirose, Masamichi
× Yamada, Mitsuhiko
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| 信州大学研究者総覧へのリンク | ||||||||||||||||||||||||
| 氏名 | Nakada, Tsutomu | |||||||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OVkhuNkh.html | |||||||||||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||||||||||
| 氏名 | Yamada, Mitsuhiko | |||||||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jhSCupca.html | |||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||
| 出版者 | PORTLAND PRESS LTD | |||||||||||||||||||||||
| 引用 | ||||||||||||||||||||||||
| 内容記述 | BIOCHEMICAL JOURNAL. 448:221-231 (2012) | |||||||||||||||||||||||
| 書誌情報 |
BIOCHEMICAL JOURNAL 巻 448, p. 221-231, 発行日 2012-12 |
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| 抄録 | ||||||||||||||||||||||||
| 内容記述 | In cardiac myocytes, LTCCs (L-type calcium channels) form a functional signalling complex with ryanodine receptors at the JM (junctional membrane). Although the specific localization of LTCCs to the JM is critical for excitation-contraction coupling. their targeting mechanism is unclear. Transient transfection of GFP (green fluorescent protein)-alpha(1S) or GFP-alpha(1C) but not P/Q-type calcium channel alpha(1A), in dysgenic (alpha(1S)-null) GLT myotubes results in correct targeting of these LTCCs to the JMs and restoration of action-potential-induced Ca2+ transients. To identify the sequences of alpha(1C) responsible for JM targeting, we generated a range of alpha(1C)-alpha(1A) chimaeras, deletion mutants and alanine substitution mutants and studied their targeting properties in GLT myotubes. The results revealed that amino acids L-1681 QAGLRTL(1688) and P(1693)EIRRAIS(1700), predicted to form two adjacent alpha-helices in the proximal C-terminus, are necessary for the JM targeting of alpha(1C). The efficiency of restoration of action-potential-induced Ca2+ transients in GLT myotubes was significantly decreased by mutations in the targeting motif. JM targeting was not disrupted by the distal C-terminus of alpha(1C) which binds to the second alpha-helix. Therefore we have identified a new structural motif in the C-terminus of alpha(1C) that mediates the targeting of cardiac LTCCs to JMs independently of the interaction between proximal and distal C-termini of alpha(1C). | |||||||||||||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||||||||||||
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| 収録物識別子タイプ | PISSN | |||||||||||||||||||||||
| 収録物識別子 | 0264-6021 | |||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA10649144 | |||||||||||||||||||||||
| PubMed | ||||||||||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||||||||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/22928916 | |||||||||||||||||||||||
| 関連名称 | 22928916 | |||||||||||||||||||||||
| 権利 | ||||||||||||||||||||||||
| 権利情報 | This research was originally published in BIOCHEMICAL JOURNAL. Nakada T. et al., The proximal C-terminus of α1C subunits is necessary for junctional membrane targeting of cardiac L-type calcium channels. Biochem. J. 2012; 448: 221-231 (C) the Biochemical Society (2012). | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||||||
| WoS | ||||||||||||||||||||||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000311878200005 | |||||||||||||||||||||||
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Nakada, Tsutomu, Flucher, Bernhard E., Kashihara, Toshihide, Sheng, Xiaona, Shibazaki, Toshihide, Horiuchi-Hirose, Miwa, Gomi, Simmon, Hirose, Masamichi, Yamada, Mitsuhiko, 2012, The proximal C-terminus of alpha(1C) subunits is necessary for junctional membrane targeting of cardiac L-type calcium channels: PORTLAND PRESS LTD, 221–231 p.
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