{"_buckets": {"deposit": "66d81489-2f88-4b17-b692-38696d0e3ca5"}, "_deposit": {"id": "3787", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "3787"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00003787", "sets": ["462"]}, "author_link": ["7035", "7036", "7037", "7038", "7039", "7040", "7041", "7042", "7043"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-12", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "231", "bibliographicPageStart": "221", "bibliographicVolumeNumber": "448", "bibliographic_titles": [{"bibliographic_title": "BIOCHEMICAL JOURNAL"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In cardiac myocytes, LTCCs (L-type calcium channels) form a functional signalling complex with ryanodine receptors at the JM (junctional membrane). Although the specific localization of LTCCs to the JM is critical for excitation-contraction coupling. their targeting mechanism is unclear. Transient transfection of GFP (green fluorescent protein)-alpha(1S) or GFP-alpha(1C) but not P/Q-type calcium channel alpha(1A), in dysgenic (alpha(1S)-null) GLT myotubes results in correct targeting of these LTCCs to the JMs and restoration of action-potential-induced Ca2+ transients. To identify the sequences of alpha(1C) responsible for JM targeting, we generated a range of alpha(1C)-alpha(1A) chimaeras, deletion mutants and alanine substitution mutants and studied their targeting properties in GLT myotubes. The results revealed that amino acids L-1681 QAGLRTL(1688) and P(1693)EIRRAIS(1700), predicted to form two adjacent alpha-helices in the proximal C-terminus, are necessary for the JM targeting of alpha(1C). The efficiency of restoration of action-potential-induced Ca2+ transients in GLT myotubes was significantly decreased by mutations in the targeting motif. JM targeting was not disrupted by the distal C-terminus of alpha(1C) which binds to the second alpha-helix. Therefore we have identified a new structural motif in the C-terminus of alpha(1C) that mediates the targeting of cardiac LTCCs to JMs independently of the interaction between proximal and distal C-termini of alpha(1C).", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "BIOCHEMICAL JOURNAL. 448:221-231 (2012)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "Nakada, Tsutomu", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.OVkhuNkh.html"}, {"subitem_link_text": "Yamada, Mitsuhiko", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.jhSCupca.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000311878200005"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "PORTLAND PRESS LTD"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "22928916"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/22928916", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1042/BJ20120773"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1042/BJ20120773", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This research was originally published in BIOCHEMICAL JOURNAL. Nakada T. et al., The proximal C-terminus of α1C subunits is necessary for junctional membrane targeting of cardiac L-type calcium channels. Biochem. 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Although the specific localization of LTCCs to the JM is critical for excitation-contraction coupling. their targeting mechanism is unclear. Transient transfection of GFP (green fluorescent protein)-alpha(1S) or GFP-alpha(1C) but not P/Q-type calcium channel alpha(1A), in dysgenic (alpha(1S)-null) GLT myotubes results in correct targeting of these LTCCs to the JMs and restoration of action-potential-induced Ca2+ transients. To identify the sequences of alpha(1C) responsible for JM targeting, we generated a range of alpha(1C)-alpha(1A) chimaeras, deletion mutants and alanine substitution mutants and studied their targeting properties in GLT myotubes. The results revealed that amino acids L-1681 QAGLRTL(1688) and P(1693)EIRRAIS(1700), predicted to form two adjacent alpha-helices in the proximal C-terminus, are necessary for the JM targeting of alpha(1C). The efficiency of restoration of action-potential-induced Ca2+ transients in GLT myotubes was significantly decreased by mutations in the targeting motif. JM targeting was not disrupted by the distal C-terminus of alpha(1C) which binds to the second alpha-helix. Therefore we have identified a new structural motif in the C-terminus of alpha(1C) that mediates the targeting of cardiac LTCCs to JMs independently of the interaction between proximal and distal C-termini of alpha(1C)."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nakada,  Tsutomu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7035", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Flucher,  Bernhard E.", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7036", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kashihara,  Toshihide", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7037", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sheng,  Xiaona", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7038", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shibazaki,  Toshihide", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7039", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Horiuchi-Hirose,  Miwa", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7040", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Gomi,  Simmon", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7041", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirose,  Masamichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7042", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamada,  Mitsuhiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7043", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Proximal_C-terminus_α1C_subunits_necessary_junctional_membrane-targeting.pdf", "filesize": [{"value": "1.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1600000.0, "url": {"label": "Proximal_C-terminus_α1C_subunits_necessary_junctional_membrane-targeting.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/3787/files/Proximal_C-terminus_α1C_subunits_necessary_junctional_membrane-targeting.pdf"}, "version_id": "ab8d516e-05fe-4e44-9afc-5cba2c293b1e"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "cardiac myocyte", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ca-v 1.2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "couplon", "subitem_subject_scheme": "Other"}, {"subitem_subject": "excitation-contraction coupling", "subitem_subject_scheme": "Other"}, {"subitem_subject": "junctional membrane complex", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "The proximal C-terminus of alpha(1C) subunits is necessary for junctional membrane targeting of cardiac L-type calcium channels", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "The proximal C-terminus of alpha(1C) subunits is necessary for junctional membrane targeting of cardiac L-type calcium channels", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["462"], "permalink_uri": "http://hdl.handle.net/10091/16907", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2013-04-01"}, "publish_date": "2013-04-01", "publish_status": "0", "recid": "3787", "relation": {}, "relation_version_is_last": true, "title": ["The proximal C-terminus of alpha(1C) subunits is necessary for junctional membrane targeting of cardiac L-type calcium channels"], "weko_shared_id": -1}