| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-04-01 |
| タイトル |
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タイトル |
Hrs Recognizes a Hydrophobic Amino Acid Cluster in Cytokine Receptors during Ubiquitin-independent Endosomal Sorting |
| 言語 |
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言語 |
eng |
| DOI |
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関連識別子 |
https://doi.org/10.1074/jbc.M110.191924 |
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関連名称 |
10.1074/jbc.M110.191924 |
| 資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Amano, Yuji
Yamashita, Yuki
Kojima, Katsuhiko
Yoshino, Kazuhisa
Tanaka, Nobuyuki
Sugamura, Kazuo
Takeshita, Toshikazu
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| 信州大学研究者総覧へのリンク |
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氏名 |
Amano, Yuji |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.jUnFumkh.html |
| 信州大学研究者総覧へのリンク |
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氏名 |
Kojima, Katsuhiko |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.OCcFuakh.html |
| 信州大学研究者総覧へのリンク |
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氏名 |
Yoshino, Kazuhisa |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.WhfFuNkh.html |
| 信州大学研究者総覧へのリンク |
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氏名 |
Takeshita, Toshikazu |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.uVLaOmSF.html |
| 出版者 |
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出版者 |
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 引用 |
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内容記述 |
JOURNAL OF BIOLOGICAL CHEMISTRY. 286(17):15458-15472 (2011) |
| 書誌情報 |
JOURNAL OF BIOLOGICAL CHEMISTRY
巻 286,
号 17,
p. 15458-15472,
発行日 2011-04-29
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| 抄録 |
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内容記述 |
Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a component of the ESCRT-0 protein complex that captures ubiquitylated cargo proteins and sorts them to the lysosomal pathway. Although Hrs acts as a key transporter for ubiquitin-dependent endosomal sorting, we previously reported that Hrs is also involved in ubiquitin-independent endosomal sorting of interleukin-2 receptor beta (IL-2R beta). Here, we show direct interactions between bacterially expressed Hrs and interleukin-4 receptor alpha (IL-4R alpha), indicating that their binding is not required for ubiquitylation of the receptors, similar to the case for IL-2R beta. Examinations of the Hrs binding regions of the receptors reveal that a hydrophobic amino acid cluster in both IL-2R beta and IL-4R alpha is essential for the binding. Whereas the wild-type receptors are delivered to LAMP1-positive late endosomes, mutant receptors lacking the hydrophobic amino acid cluster are sorted to lysobisphosphatidic acid-positive late endosomes rather than LAMP1-positive late endosomes. We also show that the degradation of these mutant receptors is attenuated. Accordingly, Hrs functions during ubiquitin-independent endosomal sorting of the receptors by recognizing the hydrophobic amino acid cluster. These findings suggest the existence of a group of cargo proteins that have this hydrophobic amino acid cluster as a ubiquitin-independent sorting signal. |
| 資源タイプ（コンテンツの種類） |
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| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0021-9258 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00251083 |
| PubMed |
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識別子タイプ |
PMID |
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関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/21362618 |
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関連名称 |
21362618 |
| 権利 |
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権利情報 |
This research was originally published in The Journal of Biological Chemistry. Amano Y, et al. Hrs Recognizes a Hydrophobic Amino Acid Cluster in Cytokine Receptors during Ubiquitin-independent Endosomal Sorting. The Journal of Biological Chemistry. 2011; 286:15458-15472. © the American Society for Biochemistry and Molecular Biology. |
| 出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000289788300074 |
HRS_RECOGNIZES_HYDROPHOBIC_AMINO_ACID_CLUSTER.pdf (4.1 MB)
