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Hepatocyte Differentiation from Human ES Cells using the Simple Embryoid Body Formation Method and the Staged-Additional Cocktail
http://hdl.handle.net/10091/15953
http://hdl.handle.net/10091/1595312bd70b8-0f1b-43be-bd21-f7915a0e0859
| 名前 / ファイル | ライセンス | アクション |
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Hepatocyte_Differentiation_Human_ES_Cells.pdf (544.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2012-09-11 | |||||
| タイトル | ||||||
| タイトル | Hepatocyte Differentiation from Human ES Cells using the Simple Embryoid Body Formation Method and the Staged-Additional Cocktail | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| DOI | ||||||
| 関連識別子 | https://doi.org/10.1100/tsw.2009.97 | |||||
| 関連名称 | 10.1100/tsw.2009.97 | |||||
| キーワード | ||||||
| 主題 | STEM-CELLS, HEPATIC DIFFERENTIATION, IN-VITRO, SPECIFICATION, ESTABLISHMENT, BODIES, LINES, LIVER | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Sasaki, Katsunori
× Sasaki, Katsunori× Ichikawa, Hinako× Takei, Shunsuke× No, Hee Sung× Tomotsune, Daihachiro× Kano, Yoshiya× Yokoyama, Tadayuki× Sirasawa, Sakiko× Mogi, Akimi× Yoshie, Susumu× Sakaki, Shujiro× Yamada, Satoshi× Matsumoto, Ken× Mizuguchi, Masahiro× Yue, Fengming× Tanaka, Yoshiki |
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| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Tomotsune, Daihachiro | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OekCuNkh.html | |||||
| 出版者 | ||||||
| 出版者 | THESCIENTIFICWORLD LTD | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | TheScientificWorldJOURNAL. 9:884-890 (2009) | |||||
| 書誌情報 |
TheScientificWorldJOURNAL 巻 9, p. 884-890, 発行日 2009 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To induce hepatocytes from human embryonic stem (hES) cells easily and effectively, a simple suspension culture method that separates ES colonies with a scraper and transfers them into newly developed, nonadherent MPC (2-methacryloyloxyethyl phosphorylcholine) plates, and the staged-additional cocktail method, including growth factors, cytokines, and Lanford serum-free medium, were developed and evaluated mainly by morphological analysis. The formed embryoid bodies (EBs) showed compact cellular agglomeration until day 4 and later formed coeloms in their interior. RT-PCR (reverse transcriptase-polymerase chain reaction) analysis showed that they are gene markers of the three germ layers. Mesenchymal cells with rough endoplasmic reticulum (rER) and extracellular matrix (ECM), and without junctions, were recognized in the interior of the EBs by transmission electron microscopy (TEM) in addition to epithelial cells. When they were stimulated by the staged-additional cocktail, they expressed albumin-positive immunoreactivity, indocyanine green (ICG) uptake, and typical ultrastructures of the hepatocytes, including bile canaliculi. These results indicate that these combined methods promote EB formation and hepatocyte differentiation from hES cells. | |||||
| 資源タイプ（コンテンツの種類） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 1537-744X | |||||
| PubMed | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/19734962 | |||||
| 関連名称 | 19734962 | |||||
| 権利 | ||||||
| 権利情報 | Copyright © 2009 Katsunori Sasaki et al. | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| WoS | ||||||
| 表示名 | Web of Science | |||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000273679300012 | |||||
