| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2011-01-17 |
| タイトル |
|
|
タイトル |
Polyclonality of BRAF Mutations in Acquired Melanocytic Nevi |
| 言語 |
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|
言語 |
eng |
| DOI |
|
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|
関連識別子 |
https://doi.org/10.1093/jnci/djp309 |
|
|
関連名称 |
10.1093/jnci/djp309 |
| 資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Lin, Jingrong
Takata, Minoru
Murata, Hiroshi
Goto, Yasufumi
Kido, Kenji
Ferrone, Soldano
Saida, Toshiaki
|
| 出版者 |
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出版者 |
OXFORD UNIV PRESS INC |
| 引用 |
|
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内容記述 |
JOURNAL OF THE NATIONAL CANCER INSTITUTE. 101(20):1423-1427 (2009) |
| 書誌情報 |
JOURNAL OF THE NATIONAL CANCER INSTITUTE
巻 101,
号 20,
p. 1423-1427,
発行日 2009-10-21
|
| 抄録 |
|
|
内容記述 |
Melanocytic nevi are thought to be senescent clones of melanocytes that have acquired an oncogenic BRAF mutation. BRAF mutation is considered to be a crucial step in the initiation of melanocyte transformation. However, using immunomagnetic separation or laser-capture microdissection, we examined BRAF mutations in sets of approximately 50 single cells isolated from acquired melanocytic nevi from 13 patients and found a substantial number of nevus cells that contained wild-type BRAF mixed with nevus cells that contained BRAF(V600E). Furthermore, we simultaneously amplified BRAF exon 15 and a neighboring single nucleotide polymorphism (SNP), rs7801086, from nevus cell samples obtained from four patients who were heterozygous for this SNP. Subcloning and sequencing of the polymerase chain reaction products showed that both SNP alleles harbored the BRAF(V600E) mutation, indicating that the same BRAF(V600E) mutation originated from different cells. The polyclonality of BRAF mutations in acquired melanocytic nevi suggests that mutation of BRAF may not be an initial event in melanocyte transformation. |
| 資源タイプ（コンテンツの種類） |
|
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0027-8874 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00702998 |
| PubMed |
|
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/19752400 |
|
|
関連名称 |
19752400 |
| 権利 |
|
|
権利情報 |
Copyright (c) The Author 2009. Published by Oxford University Press. |
| 出版タイプ |
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|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| WoS |
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|
URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000271108300011 |
Polyclonality_BRAF_mutations_Acquired_Melanocytic_Nevi.pdf (750.9 kB)
