| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2011-01-11 |
| タイトル |
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タイトル |
Reactive Oxygen Generated by NADPH Oxidase 1 (Nox1) Contributes to Cell Invasion by Regulating Matrix Metalloprotease-9 Production and Cell Migration |
| 言語 |
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言語 |
eng |
| DOI |
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関連識別子 |
https://doi.org/10.1074/jbc.M109.071779 |
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関連名称 |
10.1074/jbc.M109.071779 |
| 資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Shinohara, Masahiro
Adachi, Yoshifumi
Mitsushita, Junji
Kuwabara, Mitsuhiro
Nagasawa, Atsushi
Harada, Saori
Furuta, Shuichi
Zhang, Yugen
Seheli, Kajla
Miyazaki, Hitoshi
Kamata, Tohru
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| 出版者 |
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出版者 |
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 引用 |
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内容記述 |
JOURNAL OF BIOLOGICAL CHEMISTRY. 285(7):4481-4488 (2010) |
| 書誌情報 |
JOURNAL OF BIOLOGICAL CHEMISTRY
巻 285,
号 7,
p. 4481-4488,
発行日 2010-02-12
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| 内容記述 |
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内容記述タイプ |
Other |
|
内容記述 |
This research was originally published in J. Biol. Chem. Masahiro Shinohara, Yoshifumi Adachi, Junji Mitsushita, Mitsuhiro Kuwabara, Atsushi Nagasawa, Saori Harada, Shuichi Furuta, Yugen Zhang, Kajla Seheli, Hitoshi Miyazaki and Tohru Kamata. Enhancement of Reactive Oxygen Species Production and Chlamydial Infection by the Mitochondrial Nod-like Family Member NLRX1 . J. Biol. Chem. 2010 285: 41637-41645. Copyright (c) the American Society for Biochemistry and Molecular Biology |
| 抄録 |
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内容記述 |
A mediating role of the reactive oxygen species-generating enzyme Nox1 has been suggested for Ras oncogene transformation phenotypes including anchorage-independent cell growth, augmented angiogenesis, and tumorigenesis. However, little is known about whether Nox1 signaling regulates cell invasiveness. Here, we report that the cell invasion activity was augmented in K-Ras-transformed normal rat kidney cells and attenuated by transfection of Nox1 small interference RNAs(siRNAs) into the cells. Diphenyleneiodonium (DPI) or Nox1 siRNAs blocked up-regulation of matrix metalloprotease-9 at both protein and mRNA levels in K-Ras-transformed normal rat kidney cells. Furthermore, DPI and Nox1 siRNAs inhibited the activation of IKK alpha kinase and the degradation of I kappa B alpha, suppressing the NF kappa B-dependent matrix metalloprotease-9 promoter activity. Additionally, epidermal growth factor-stimulated migration of CaCO-2 cells was abolished by DPI and Nox1 siRNAs, indicating the requirement of Nox1 activity for the motogenic effect of epidermal growth factor. This Nox1 action was mediated by down-regulation of the Rho activity through the low molecular weight protein-tyrosine phosphatase-p190RhoGAP-dependent mechanism. Taken together, our findings define a mediating role of Nox1-generated reactive oxygen species in cell invasion processes, most notably metalloprotease production and cell motile activity. |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0021-9258 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00251083 |
| PubMed |
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識別子タイプ |
PMID |
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関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/20018867 |
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関連名称 |
20018867 |
| 権利 |
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権利情報 |
Copyright (c) 2010 The American Society for Biochemistry and Molecular Biology, Inc. |
| 出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000275274800026 |
Reactive_Oxygen_Generated_NADPH_Oxidase1.pdf (1.2 MB)
