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  1. 050 医学部, 大学院医学系研究科
  2. 0501 学術論文

Scapinin, the Protein Phosphatase 1 Binding Protein, Enhances Cell Spreading and Motility by Interacting with the Actin Cytoskeleton

http://hdl.handle.net/10091/10807
http://hdl.handle.net/10091/10807
c1cb4f1c-49a1-4344-babb-361381c6c261
名前 / ファイル ライセンス アクション
Scapinin_Protein_Phosphatase.pdf Scapinin_Protein_Phosphatase.pdf (478.0 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2010-11-30
タイトル
タイトル Scapinin, the Protein Phosphatase 1 Binding Protein, Enhances Cell Spreading and Motility by Interacting with the Actin Cytoskeleton
言語
言語 eng
DOI
関連識別子 https://doi.org/10.1371/journal.pone.0004247
関連名称 10.1371/journal.pone.0004247
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Sagara, Junji

× Sagara, Junji

en Sagara, Junji

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Arata, Toshiaki

× Arata, Toshiaki

en Arata, Toshiaki

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Taniguchi, Shunichiro

× Taniguchi, Shunichiro

en Taniguchi, Shunichiro

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信州大学研究者総覧へのリンク
氏名 Taniguchi, Shunichiro
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HCyUHCDV.html
出版者
出版者 PUBLIC LIBRARY SCIENCE
引用
内容記述 PLOS ONE. 4(1):e4247 (2009)
書誌情報 PLOS ONE

巻 4, 号 1, 発行日 2009-01-22
内容記述
内容記述タイプ Other
内容記述 Copyright (c) 2009 Sagara et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
抄録
内容記述 Scapinin, also named phactr3, is an actin and protein phosphatase 1 (PP1) binding protein, which is expressed in the adult brain and some tumor cells. At present, the role(s) of scapinin in the brain and tumors are poorly understood. We show that the RPEL-repeat domain of scapinin, which is responsible for its direct interaction with actin, inhibits actin polymerization in vitro. Next, we established a Hela cell line, where scapinin expression was induced by tetracycline. In these cells, expression of scapinin stimulated cell spreading and motility. Scapinin was colocalized with actin at the edge of spreading cells. To explore the roles of the RPEL-repeat and PP1-binding domains, we expressed wild-type and mutant scapinins as fusion proteins with green fluorescence protein (GFP) in Cos7 cells. Expression of GFP-scapinin (wild type) also stimulated cell spreading, but mutation in the RPEL-repeat domain abolished both the actin binding and the cell spreading activity. PP1-binding deficient mutants strongly induced cell retraction. Long and branched cytoplasmic processes were developed during the cell retraction. These results suggest that scapinin enhances cell spreading and motility through direct interaction with actin and that PP1 plays a regulatory role in scapinin-induced morphological changes.
資源タイプ(コンテンツの種類)
ISSN
収録物識別子タイプ PISSN
収録物識別子 1932-6203
PubMed
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/19158953
関連名称 19158953
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
WoS
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000265482200004
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Cite as

Sagara, Junji, Arata, Toshiaki, Taniguchi, Shunichiro, 2009, Scapinin, the Protein Phosphatase 1 Binding Protein, Enhances Cell Spreading and Motility by Interacting with the Actin Cytoskeleton: PUBLIC LIBRARY SCIENCE.

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