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The search for an understanding of the causes of genetic variants and mutations has resulted in the development of a simple laboratory technique, known as the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, for the detection of single nucleotide polymorphisms (SNPs). PCR-RFLP allows rapid detection of point mutations after the genomic sequences are amplified by PCR. The mutation is discriminated by digestion with specific restriction endonucleases and is identified by gel electrophoresis after staining with ethidium bromide (EtBr). This convenient and simple method is inexpensive and accurate for SNP genotyping and especially useful in small basic research studies of complex genetic diseases. 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  1. 050 医学部, 大学院医学系研究科
  2. 0501 学術論文

Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism

http://hdl.handle.net/10091/1014
06353564-6549-4ab1-8514-7e311337cc05
名前 / ファイル ライセンス アクション
SNP_detection.pdf SNP_detection.pdf (263.0 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-03-31
タイトル
言語 en
タイトル Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Ota, M

× Ota, M

WEKO 8451

en Ota, M

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Fukushima, H

× Fukushima, H

WEKO 8452

en Fukushima, H

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Kulski, JK

× Kulski, JK

WEKO 8453

en Kulski, JK

Search repository
Inoko, H

× Inoko, H

WEKO 8454

en Inoko, H

Search repository
信州大学研究者総覧へのリンク
氏名 Ota, M
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.ZefVOFkh.html
出版者
出版者 NATURE PUBLISHING GROUP
引用
内容記述タイプ Other
内容記述 NATURE PROTOCOLS. 2(11): 2857-2864 (2007)
書誌情報 NATURE PROTOCOLS

巻 2, 号 11, p. 2857-2864, 発行日 2007
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ PISSN
収録物識別子 1745-2473
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11976000
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1038/nprot.2007.407
関連名称
関連名称 10.1038/nprot.2007.407
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000253140000022
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