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The search for an understanding of the causes of genetic variants and mutations has resulted in the development of a simple laboratory technique, known as the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, for the detection of single nucleotide polymorphisms (SNPs). PCR-RFLP allows rapid detection of point mutations after the genomic sequences are amplified by PCR. The mutation is discriminated by digestion with specific restriction endonucleases and is identified by gel electrophoresis after staining with ethidium bromide (EtBr). This convenient and simple method is inexpensive and accurate for SNP genotyping and especially useful in small basic research studies of complex genetic diseases. The whole protocol takes only a day to carry out."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ota,  M", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8451", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fukushima,  H", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8452", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kulski,  JK", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8453", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Inoko,  H", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8454", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "SNP_detection.pdf", "filesize": [{"value": "263.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 263000.0, "url": {"label": "SNP_detection.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/3987/files/SNP_detection.pdf"}, "version_id": "5416f521-329d-469b-868d-541a004025c0"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["461/462"], "permalink_uri": "http://hdl.handle.net/10091/1014", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-03-31"}, "publish_date": "2008-03-31", "publish_status": "0", "recid": "3987", "relation": {}, "relation_version_is_last": true, "title": ["Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism"], "weko_shared_id": -1}