{"_buckets": {"deposit": "1e79803d-10d4-4b6d-9331-681b75038c05"}, "_deposit": {"id": "3988", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "3988"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00003988", "sets": ["462"]}, "author_link": ["8455", "8456", "8457", "8458"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-06-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "11", "bibliographicPageEnd": "4163", "bibliographicPageStart": "4157", "bibliographicVolumeNumber": "103", "bibliographic_titles": [{"bibliographic_title": "BLOOD"}]}]}, "item_6_description_19": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "This research was originally published in Blood. Authors. Okumura, N; Gorkun, OV; Terasawa, F; Lord, ST Title. Blood. 2004; 103(12): 4157-4163. © by the American Society of Hematology.", "subitem_description_type": "Other"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_31": {"attribute_name": "フォーマット：mimeタイプ", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "BLOOD. 103(11): 4157-4163 (2004)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "Okumura, N", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.OVnmgCkh.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000221657600032"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "AMER SOC HEMATOLOGY"}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1182/blood.-2003-12-4296"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1182/blood.-2003-12-4296", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0006-4971", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0006-4971", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00567156", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "CALCIUM-BINDING SITE; GLY-HIS-ARG; FRAGMENT-D; IMPAIRED POLYMERIZATION; VLISSINGEN/FRANKFURT IV; RECOMBINANT FIBRINOGENS; ABNORMAL FIBRINOGEN; CRYSTAL-STRUCTURES; BLOOD-COAGULATION; MATSUMOTO-II"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Crystallographic structures indicate that gamma-chain residue Asn308 participates in D:D interactions and indeed substitutions of gammaAsn308 with lysine or isoleucine have been identified in dysfibrinogens with impaired polymerization. To probe the role of Asn308 in polymerization, we synthesized 3 variant fibrinogens: gammaAsn308 changed to lysine (gammaN308K), isoleucine (gammaN3081), and alanine (gammaN308A). We measured thrombin-catalyzed polymerization by turbidity, fibrinopeptide release by high-performance liquid chromatography, and factor Xilla-catalyzed crosslinking by sodium dodecyl sulfate-polyacryl-amide gel electrophoresis. In the absence of added calcium, polymerization was clearly impaired with all 3 variants. In contrast, at 0.1 mM calcium, only polymerization of gammaN3081\u003c remained markedly abnormal. The release of thrombin-catalyzed fibrinopeptide B (FpB) was delayed in the absence of calcium, whereas at 1 mM calcium FpB release was delayed only with gammaN308K. Factor Alla-catalyzed gamma-gamma dimer formation was delayed with fibrinogen (in absence of thrombin), whereas with fibrin (in presence of thrombin) gamma-gamma dimer formation of only gammaN308K was delayed. These data corroborate the recognized link between FpB release and polymerization. They show fibrin cross-link formation likely depends on the structure of protofibrils. Together, our results show substitution of Asn308 with a hydrophobic residue altered neither polymer formation nor polymer structure at physiologic calcium concentrations, whereas substitution with lysine altered both. (C) 2004 by The American Society of Hematology"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Okumura,  N", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8455", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Gorkun,  OV", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8456", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Terasawa,  F", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8457", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Lord,  ST", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8458", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "r308Variant(BLOOD)+Fig.pdf", "filesize": [{"value": "595.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 595400.0, "url": {"label": "r308Variant(BLOOD)+Fig.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/3988/files/r308Variant(BLOOD)+Fig.pdf"}, "version_id": "c93dbb7a-a092-4e3b-8f08-7260881a1faf"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Substitution of the gamma-chain Asn308 disturbs the D : D interface affecting fibrin polymerization, fibrinopeptide B release, and FXIIIa-catalyzed cross-linking", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Substitution of the gamma-chain Asn308 disturbs the D : D interface affecting fibrin polymerization, fibrinopeptide B release, and FXIIIa-catalyzed cross-linking", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["462"], "permalink_uri": "http://hdl.handle.net/10091/1011", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-03-27"}, "publish_date": "2008-03-27", "publish_status": "0", "recid": "3988", "relation": {}, "relation_version_is_last": true, "title": ["Substitution of the gamma-chain Asn308 disturbs the D : D interface affecting fibrin polymerization, fibrinopeptide B release, and FXIIIa-catalyzed cross-linking"], "weko_shared_id": -1}