{"_buckets": {"deposit": "5ca0505b-d15d-4813-8bd8-9f69724dda23"}, "_deposit": {"id": "3989", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "3989"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00003989", "sets": ["461:462"]}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageEnd": "1367", "bibliographicPageStart": "1359", "bibliographicVolumeNumber": "2", "bibliographic_titles": [{"bibliographic_title": "JOURNAL OF THROMBOSIS AND HAEMOSTASIS"}]}]}, "item_6_description_19": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "This is an electronic version of an Article published in Journal of Thrombosis and Haemostasis 2004; 2(8): 1359-1367", "subitem_description_type": "Other"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_31": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\uff1amime\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "JOURNAL OF THROMBOSIS AND HAEMOSTASIS. 2(8): 1359-1367 (2004)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Suzuki, T", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.HaLpuUkh.html"}, {"subitem_link_text": "Okumura, N", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.OVnmgCkh.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000223286700025"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "BLACKWELL PUBL LTD"}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1111/j.1538-7836.2004.00844.x"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.1111/j.1538-7836.2004.00844.x", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1538-7933", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1538-7933", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11811179", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "D : D interface; fibrin polymerization; recombinant fibrinogen; variant fibrinogen; XIIIa-catalyzed cross-linking of fibrin"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "IMPAIRED POLYMERIZATION; TOKYO-II; DYSFIBRINOGENS; PURIFICATION; SUBSTITUTION; CALCIUM; DOMAIN; SITES; CHAIN; ARG"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Background and objectives: We have previously reported that recombinant gamma275Cys fibrinogen exhibits a marked impairment of functions as well as aberrant fibrin clot and bundle structures, as compared with wild-type, gamma275Arg, and plasma fibrinogen from a heterozygous proband. Since gammaArg275His mutations have also been reported in 10 families, we synthesized recombinant gamma275His fibrinogen and gamma275Ala fibrinogen (as a control) and analyzed and compared them with gamma275Cys and gamma275Arg. Methods: A variant gamma-chain expression plasmid was transfected into Chinese hamster ovary cells expressing normal human fibrinogen Aalpha- and Bbeta-chains. After purification of the recombinant variant fibrinogens, we performed functional analyzes for thrombin-catalyzed fibrin polymerization and factor XIIIa (FXIIIa)-catalyzed gamma-gamma dimer formation from fibrin or fibrinogen and also ultrastructural analysis of fibrin clots and bundles. Results: By comparison with both gamma275His and gamma275Ala fibrinogens, recombinant gamma275Cys fibrinogen exhibited a more impaired gamma-gamma dimer formation from fibrin or fibrinogen, a more aberrant fibrin clot structure, and thicker fibers in fibrin bundles. In 1:1 mixtures of gamma275Arg and gamma275Cys fibrinogens or gamma275Arg and gamma275His fibrinogens, thrombin-catalyzed fibrin polymerization and both fibrin clot and fiber structures showed some compensation (as compared with gamma275Cys or gamma275His alone). Conclusion: These results strongly suggest that an amino acid substitution of gamma275Arg alone disrupts D:D interactions in thrombin -catalyzed fibrin polymerization and the formation of fibrin bundles and fibrin clots. Moreover, the existence of a subsequent disulfide-linked Cys in gamma275C fibrinogen augments the impairment caused by a His or Ala substitution."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hirota-Kawadobora,  M"}], "nameIdentifiers": [{"nameIdentifier": "8459", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Terasawa,  F"}], "nameIdentifiers": [{"nameIdentifier": "8460", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki,  T"}], "nameIdentifiers": [{"nameIdentifier": "8461", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tozuka,  M"}], "nameIdentifiers": [{"nameIdentifier": "8462", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sano,  K"}], "nameIdentifiers": [{"nameIdentifier": "8463", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okumura,  N"}], "nameIdentifiers": [{"nameIdentifier": "8464", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "\uff52\uff52275CHA-FIG.pdf", "filesize": [{"value": "2.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2200000.0, "url": {"label": "\uff52\uff52275CHA-FIG.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/3989/files/\uff52\uff52275CHA-FIG.pdf"}, "version_id": "bb5816e3-1567-4241-88d0-3e08cb99ff0e"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Comparison of thrombin-catalyzed fibrin polymerization and Factor XIIIa-catalyzed cross-linking of fibrin among three recombinant variant fibrinogens, gamma 275C, gamma 275H, and gamma 275A", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Comparison of thrombin-catalyzed fibrin polymerization and Factor XIIIa-catalyzed cross-linking of fibrin among three recombinant variant fibrinogens, gamma 275C, gamma 275H, and gamma 275A"}]}, "item_type_id": "6", "owner": "1", "path": ["461/462"], "permalink_uri": "http://hdl.handle.net/10091/1010", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2008-03-27"}, "publish_date": "2008-03-27", "publish_status": "0", "recid": "3989", "relation": {}, "relation_version_is_last": true, "title": ["Comparison of thrombin-catalyzed fibrin polymerization and Factor XIIIa-catalyzed cross-linking of fibrin among three recombinant variant fibrinogens, gamma 275C, gamma 275H, and gamma 275A"], "weko_shared_id": 3}