{"_buckets": {"deposit": "bf93905e-fb63-4714-b5e0-2bbf9bd33394"}, "_deposit": {"id": "3997", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "3997"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00003997", "sets": ["462"]}, "author_link": ["8498", "8499", "8500", "8501", "8502"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "155", "bibliographicPageStart": "149", "bibliographicVolumeNumber": "17", "bibliographic_titles": [{"bibliographic_title": "Transgenic research"}]}]}, "item_6_description_19": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "The original publication is available at www.springerlink.com.", "subitem_description_type": "Other"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_31": {"attribute_name": "フォーマット：mimeタイプ", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "TRANSGENIC RESEARCH. 17(1): 149-155 (2008)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "Shindo, T", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.OCLpOayV.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000251869000013"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "SPRINGER"}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1007/s11248-007-9134-7"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1007/s11248-007-9134-7", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0962-8819", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0962-8819", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10873487", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "crude DNA solution; real-time PCR; SYBR green; zygosity; transgenic mice; gene-targeted mice"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "QUANTITATIVE PCR; TRANSGENIC MICE; ACCURATE DETERMINATION; MOUSE EMBRYOS; COPY NUMBERS; EXPRESSION; CURVES"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "We examined whether crude DNA extracts prepared from gene-engineered mouse tissues are suitable as a template for zygosity determination by SYBR Green real-time genomic PCR. A crude DNA solution was prepared by brief incubation with lysis buffer containing ear, tail, or fetus of ROSA26 mouse, a gene-trapped strain carrying the beta-galactosidase (beta-gal) gene. Five serially diluted crude DNA samples (original, 2-, 4-, 8-, 16-diluted) were next prepared and then subjected to three-step (95 degrees C, 60 degrees C and 72 degrees C) reactions of real-time PCR to detect the beta-gal gene and the receptor-activity-modifying protein 3 (ramp3) gene (as an internal reference gene). The slopes of standard curves obtained from the real-time PCR indicated that amplification efficiency was approximately 99%, and the efficiencies of target and reference were almost equal. With this system, we next determined the zygosity of mice derived from mating heterozygous ROSA26 females and males, and found a sharp distinction in zygosity, wild-type, heterozygous and homozygous. Assessment of crude DNA samples from other gene-engineered mice including B6ZP3Cre-Tg, B6rAM-Tg, and Ramp2-gene-targeted strains revealed that our method was effective for determination of zygosity. The present method is more convenient and rapid than formerly published methods employing purified genomic DNA as a template. Our method will be particularly useful for experiments requiring rapid and accurate genotyping of gene-modified animals/fetuses."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sakurai,  T", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8498", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kamiyoshi,  A", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8499", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe,  S", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8500", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato,  M", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8501", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shindo,  T", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8502", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "zygosity_determination.pdf", "filesize": [{"value": "319.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 319100.0, "url": {"label": "zygosity_determination.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/3997/files/zygosity_determination.pdf"}, "version_id": "a795f7c3-ea4a-4b07-8aee-9daae3f2e16a"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Rapid zygosity determination in mice by SYBR Green real-time genomic PCR of a crude DNA solution", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Rapid zygosity determination in mice by SYBR Green real-time genomic PCR of a crude DNA solution", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["462"], "permalink_uri": "http://hdl.handle.net/10091/974", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-05"}, "publish_date": "2008-02-05", "publish_status": "0", "recid": "3997", "relation": {}, "relation_version_is_last": true, "title": ["Rapid zygosity determination in mice by SYBR Green real-time genomic PCR of a crude DNA solution"], "weko_shared_id": -1}