{"_buckets": {"deposit": "3408a3ce-a03c-419b-ac01-a9f5a975184c"}, "_deposit": {"id": "4044", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "4044"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00004044", "sets": ["462"]}, "author_link": ["8809", "8810", "8811", "8812", "8813", "8814"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "99", "bibliographicPageStart": "91", "bibliographicVolumeNumber": "43", "bibliographic_titles": [{"bibliographic_title": "HEPATOLOGY"}]}]}, "item_6_description_19": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Copyright © 2006 American Association for the Study of Liver Diseases", "subitem_description_type": "Other"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_31": {"attribute_name": "フォーマット：mimeタイプ", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "HEPATOLOGY. 43(1): 91-99 (2006)", "subitem_description_type": "Other"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000234460000014"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "JOHN WILEY \u0026 SONS INC"}]}, "item_6_relation_46": {"attribute_name": "他の資源との関係：URI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "http://www3.interscience.wiley.com/cgi-bin/jhome/106570044"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www3.interscience.wiley.com/cgi-bin/jhome/106570044", "subitem_relation_type_select": "URI"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1002/hep.20917"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1002/hep.20917", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0270-9139", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0270-9139", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10620324", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "IMMUNOGLOBULIN-M ANTIBODY; T-LYMPHOCYTE RESPONSE; IMMUNE-RESPONSES; VIRAL CLEARANCE; NEUTRALIZING ANTIBODY; INTERFERON-ALPHA; SURFACE-ANTIGEN; LIVER-DISEASE; ELISPOT ASSAY; CORE PROTEIN"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "To investigate the quantitative characteristics of humoral immunity in patients with hepatitis C, we established an enzyme-linked immunosorbent spot (ELISpot) assay for detection of anti-hepatitis C virus (HCV)-secreting B cells. Receiver operating characteristic curve analysis demonstrated 100% specificity and 58% to 92% sensitivity for detecting B-cell responses to NS5b, NS3, E2, and core antigens. The median sum of anti-HCV-secreting B cells to all HCV antigens tested was significantly higher in 39 patients with chronic hepatitis C (47.3 spot forming cells [SFCs]/10(6) peripheral blood mononuclear cells [PBMCs]) than in 9 recovered subjects (15.3 SFCs/10(6) PBMCs; P = .05) or 11 uninfected controls (5-3 SFCs/10(6) PBMCs; P \u003c .001); the significant difference (P = .018) in chronic versus recovered patients was in reactivity to nonstructural antigens NS3 and NS5b. Anti-HCV immunoglubulin M (IgM)-secreting B cells were also readily detected and persisted decades into HCV infection; there was no difference in IgM-positive cells between chronic and recovered patients. ELISpot reactivity to genotype 1-derived antigens was equivalent in patients of genotypes 1, 2, and 3. There was significant correlation between the numbers of anti-HCV IgG-secreting B cells and serum aminotransferase and to the level of circulating antibody. In conclusion, ELISpot assays can be adapted to study B-cell as well as T-cell responses to HCV. Measurement at the single-cell level suggests that humoral immunity plays a minor role in recovery from HCV infection and that B-cell immunity is strongest in those with persistent infection."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Umemura,  T", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8809", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Wang,  RYH", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8810", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Schechterly,  C", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8811", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shih,  JWK", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8812", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kiyosawa,  K", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8813", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Alter,  HJ", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8814", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Quantitave analysis of anti-hepatitis c.pdf", "filesize": [{"value": "225.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 225900.0, "url": {"label": "Quantitave analysis of anti-hepatitis c.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/4044/files/Quantitave analysis of anti-hepatitis c.pdf"}, "version_id": "745a230e-5377-4f4b-ba31-0127121b299b"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Quantitative analysis of anti-hepatitis C virus anti body-secreting B cells in patients with chronic hepatitis C", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Quantitative analysis of anti-hepatitis C virus anti body-secreting B cells in patients with chronic hepatitis C", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["462"], "permalink_uri": "http://hdl.handle.net/10091/228", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-05-15"}, "publish_date": "2007-05-15", "publish_status": "0", "recid": "4044", "relation": {}, "relation_version_is_last": true, "title": ["Quantitative analysis of anti-hepatitis C virus anti body-secreting B cells in patients with chronic hepatitis C"], "weko_shared_id": -1}