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Analysis of plasma or purified fibrinogen from the heterozygous propositus revealed that the amount of variant gamma-chain was lower than that of normal gamma-chain. In order to examine the basis for this difference, we transfected Chinese hamster ovary cells and established stable cell lines that expressed both chains, gamma Delta/gamma N, only the normal chain, gamma N, and only the variant chain, gamma Delta. We measured fibrinogen concentration of confluent cultures by ELISA. We found the ratios of the concentrations in the media to the concentrations in the cell lysates of gamma Delta, gamma Delta/gamma N, and gamma N-cells were 0.42, 0.60, and 1.00, respectively. We measured the concentrations of the gamma Delta and gamma N chains by densitometric analysis of samples following separation by SIDS-PAGE and found the fraction of gamma Delta-chains in cell lysates was always greater than the fraction in the respective culture media. We examined the kinetics of fibrinogen synthesis, assembly and secretion in pulse-chase experiments,and found that the gamma Delta-chain was assembled into intact fibrinogen at a rate similar to assembly of the gamma N-chain into normal fibrinogen, but was secreted into the medium at a slightly slower rate than normal fibrinogen. Considered together, these experiments indicate secretion of the variant fibrinogen was slightly impaired. These results suggest that the reduced level of gamma Delta 319,320 fibrinogen in the plasma of the Otsu patient arises from modestly impaired secretion of this variant fibrinogen."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kani, S", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8959", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Terasawa, F", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8960", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Lord, ST", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8961", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tozuka, M", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8962", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ota, H", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8963", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okumura, N", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8964", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Katsuyama, T", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8965", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Rv-Kani.pdf", "filesize": [{"value": "636.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 636100.0, "url": {"label": "Rv-Kani.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/4061/files/Rv-Kani.pdf"}, "version_id": "6b721546-b87a-44f7-a12a-26d328ff0efe"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "In vitro expression demonstrates impaired secretion of the gamma Asn319, Asp320 deletion variant fibrinogen", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "In vitro expression demonstrates impaired secretion of the gamma Asn319, Asp320 deletion variant fibrinogen", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["462"], "permalink_uri": "http://hdl.handle.net/10091/193", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-04-25"}, "publish_date": "2007-04-25", "publish_status": "0", "recid": "4061", "relation": {}, "relation_version_is_last": true, "title": ["In vitro expression demonstrates impaired secretion of the gamma Asn319, Asp320 deletion variant fibrinogen"], "weko_shared_id": -1}
In vitro expression demonstrates impaired secretion of the gamma Asn319, Asp320 deletion variant fibrinogen
http://hdl.handle.net/10091/193
http://hdl.handle.net/10091/1935ce314c2-21d7-4047-ab2d-8d6e21e41cf4
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-04-25 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | In vitro expression demonstrates impaired secretion of the gamma Asn319, Asp320 deletion variant fibrinogen | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Kani, S
× Kani, S× Terasawa, F× Lord, ST× Tozuka, M× Ota, H× Okumura, N× Katsuyama, T |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Ota, H | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.WNkCHhkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Okumura, N | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OVnmgCkh.html | |||||
出版者 | ||||||
出版者 | SCHATTAUER GMBH-VERLAG MEDIZIN NATURWISSENSCHAFTEN | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | THROMBOSIS AND HAEMOSTASIS. 94(1): 53-59 (2005) | |||||
書誌情報 |
Thrombosis and Haemostasis 巻 94, 号 1, p. 53-59, 発行日 2005-07 |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | This article is not an exact copy of the original published article in [THROMBOSIS AND HAEMOSTASIS]. The definitive publisher-authenticated version of [THROMBOSIS AND HAEMOSTASIS 94;53-59,2005] is available online at: [http://www.schattauer.de/] | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0340-6245 | |||||
書誌レコードID | ||||||
識別子タイプ | NCID | |||||
関連識別子 | BA20605267 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1160/TH05-02-0134 | |||||
関連名称 | 10.1160/TH05-02-0134 | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
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