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Blood culture is one of the most important tests to detect and identify the pathogens causing BSI. In this study, we performed a retrospective case series analysis of 644 laboratoryconfirmed episodes of bacteraemia at Shinshu University Hospital between 1 July 2001 and 31 December 2005. Although bacterial culture examinations decreased in number during these 5 years, blood cultures gradually increased. Positive rates went up from 8.1\uff05 to 12.6\uff05. The most commonly isolated microorganisms were coagulase-negative staphylococci (CNS)(23.1\uff05), Staphylococcus aureus (14.7\uff05), Klebsiella species(9.3\uff05) and Escherichia coli (9.1\uff05). Staphylococcus species accounted for 37.8\uff05 of all microorganisms detected. The proportion of the microorganisms did not change remarkably during the study period. The microorganisms commonly cultured were different among the departments because infected sites were specific for each department. 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