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  1. 050 医学部, 大学院医学系研究科
  2. 0507 学位論文
  3. 博士(医学)

Differentiation of Monkey Embryonic Stem Cells to Hepatocytes by Feeder-Free Dispersion Culture and Expression Analyses of Cytochrome P450 Enzymes Responsible for Drug Metabolism

http://hdl.handle.net/10091/17667
6ac42003-34fa-4945-bcb7-7d4bcc2d2c89
名前 / ファイル ライセンス アクション
H24Otsu1155_Maruyama.pdf H24Otsu1155_Maruyama.pdf (2.1 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-06-27
タイトル
言語 en
タイトル Differentiation of Monkey Embryonic Stem Cells to Hepatocytes by Feeder-Free Dispersion Culture and Expression Analyses of Cytochrome P450 Enzymes Responsible for Drug Metabolism
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Maruyama, Junya

× Maruyama, Junya

WEKO 17324

Maruyama, Junya

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Matsunaga, Tamihide

× Matsunaga, Tamihide

WEKO 17325

Matsunaga, Tamihide

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Yamaori, Satoshi

× Yamaori, Satoshi

WEKO 17326

Yamaori, Satoshi

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Sakamoto, Sakae

× Sakamoto, Sakae

WEKO 17327

Sakamoto, Sakae

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Kamada, Noboru

× Kamada, Noboru

WEKO 17328

Kamada, Noboru

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Nakamura, Katsunori

× Nakamura, Katsunori

WEKO 17329

Nakamura, Katsunori

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Kikuchi, Shinji

× Kikuchi, Shinji

WEKO 17330

Kikuchi, Shinji

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Ohmori, Shigeru

× Ohmori, Shigeru

WEKO 17331

Ohmori, Shigeru

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出版者
出版者 PHARMACEUTICAL SOC JAPAN
引用
内容記述タイプ Other
内容記述 BIOLOGICAL & PHARMACEUTICAL BULLETIN. 36(2) 292-298 (2013)
書誌情報 BIOLOGICAL & PHARMACEUTICAL BULLETIN

巻 36, 号 2, p. 292-298, 発行日 2013-02
内容記述
内容記述タイプ Other
内容記述 信州大学博士(医学)・学位論文・平成25年2月13日授与(乙第1155号)・丸山 順也
抄録
内容記述タイプ Abstract
内容記述 We reported previously that monkey embryonic stem cells (ESCs) were differentiated into hepatocytes by formation of embryoid bodies (EBs). However, this EB formation method is not always efficient for assays using a large number of samples simultaneously. A dispersion culture system, one of the differentiation methods without EB formation, is able to more efficiently provide a large number of feeder-free undifferentiated cells. A previous study demonstrated the effectiveness of the Rho-associated kinase inhibitor Y-27632 for feeder-free dispersion culture and induction of differentiation of monkey ESCs into neural cells. In the present study, the induction of differentiation of cynomolgus monkey ESCs (cmESCs) into hepatocytes was performed by the dispersion culture method, and the expression and drug inducibility of cytochrome P450 (CYP) enzymes in these hepatocytes were examined. The cmESCs were successfully differentiated into hepatocytes under feeder-free dispersion culture conditions supplemented with Y-27632. The hepatocytes differentiated from cmESCs expressed the mRNAs for three hepatocyte marker genes (alpha-fetoprotein, albumin, CYP7A1) and several CYP enzymes, as measured by real-time polymerase chain reaction. In particular, the basal expression of cmCYP3A4 (3A8) in these hepatocytes was detected at mRNA and enzyme activity (testosterone 6 beta-hydroxylation) levels. Furthermore, the expression and activity of cmCYP3A4 (3A8) were significantly upregulated by rifampicin. These results indicated the effectiveness of Y-27632 supplementation for feeder-free dispersed culture and induction of differentiation into hepatocytes, and the expression of functional CYP enzyme(s) in cmESC-derived hepatic cells.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1248/bpb.b12-00866
関連名称
関連名称 10.1248/bpb.b12-00866
権利
権利情報 Copyright (c) 2013 The Pharmaceutical Society of Japan
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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