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Objective: The aim of the study was to determine if cells derived from HAMCs support the structural and functional reconstruction of freeze-injured mouse bladders. Design, setting, and participants: HAMCs were harvested from an amnion membrane, and cells were cultured for 7 d prior to injection into the freeze-injured bladder walls of nude mice. Intervention: Three days prior to implantation, the posterior bladder walls were freeze injured for 30 s. The cultured HAMC-derived cells (0.5 x 10(5) cells per 50 mu l) were implanted into the injured regions. Control bladders received a cell-free injection. At 1, 2, 4, and 6 wk after the cell implantation, the experimental bladders were extirpated. Measurements: The bladder tissues were examined by immunohistochemistry for alpha-smooth muscle actin (SMA). The HAMC-derived cells were detected by antihuman nuclei antibody (HuNu). Separately, bladder muscle strips were examined for contractile responses to potassium. 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Objective: The aim of the study was to determine if cells derived from HAMCs support the structural and functional reconstruction of freeze-injured mouse bladders. Design, setting, and participants: HAMCs were harvested from an amnion membrane, and cells were cultured for 7 d prior to injection into the freeze-injured bladder walls of nude mice. Intervention: Three days prior to implantation, the posterior bladder walls were freeze injured for 30 s. The cultured HAMC-derived cells (0.5 x 10(5) cells per 50 mu l) were implanted into the injured regions. Control bladders received a cell-free injection. At 1, 2, 4, and 6 wk after the cell implantation, the experimental bladders were extirpated. Measurements: The bladder tissues were examined by immunohistochemistry for alpha-smooth muscle actin (SMA). The HAMC-derived cells were detected by antihuman nuclei antibody (HuNu). Separately, bladder muscle strips were examined for contractile responses to potassium. Results and limitations: At 1 wk after implantation, the HAMC-derived cells, which were detected by HuNu, differentiated into muscular layers composed of SMA-positive cells. From 2 to 6 wk after implantation, abundant layers of SMA-positive and HuNu-positive cells developed. In control bladders, few SMA-positive cells remained at the injured regions at 1 wk, but by 6 wk, more were present. At 1 wk, the contractile responses to potassium of the cell-implanted bladders were significantly higher than those of the control-injected ones. Control-injected bladders also recovered by 6 wk, but the rate of recovery was slower. Conclusions: Freeze-injured mouse bladders implanted with HAMC-derived cells recovered morphology and function faster than control-injected bladders. (C) 2009 European Association of Urology. Published by Elsevier B. V. 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Differentiation of Smooth Muscle Cells from Human Amniotic Mesenchymal Cells Implanted in the Freeze-Injured Mouse Urinary Bladder
http://hdl.handle.net/10091/16879
http://hdl.handle.net/10091/16879a1625110-2a28-49f5-9685-5353520d006c
名前 / ファイル | ライセンス | アクション |
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Differentiation_Smooth_Muscle_Cells_Human_Amniotic_Mesenchymal_Cells.pdf (1.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-04-01 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Differentiation of Smooth Muscle Cells from Human Amniotic Mesenchymal Cells Implanted in the Freeze-Injured Mouse Urinary Bladder | |||||
言語 | ||||||
言語 | eng | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.eururo.2009.12.031 | |||||
関連名称 | 10.1016/j.eururo.2009.12.031 | |||||
キーワード | ||||||
主題 | Regeneration, Cell transplantation, Mice, Urinary bladder, Bladder injury | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Minagawa, Tomonori
× Minagawa, Tomonori× Imamura, Tetsuya× Igawa, Yasuhiko× Aizawa, Naoki× Ishizuka, Osamu× Nishizawa, Osamu |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Minagawa, Tomonori | |||||
URL | https://soar-rd.shinshu-u.ac.jp/profile/ja.jCfagUkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Imamura, Tetsuya | |||||
URL | https://soar-rd.shinshu-u.ac.jp/profile/ja.ypACOmyC.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Nishizawa, Osamu | |||||
URL | https://soar-rd.shinshu-u.ac.jp/profile/ja.WeyeWmyU.html | |||||
出版者 | ||||||
出版者 | ELSEVIER SCIENCE BV | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | EUROPEAN UROLOGY. 58(2):299-306 (2010) | |||||
書誌情報 |
EUROPEAN UROLOGY 巻 58, 号 2, p. 299-306, 発行日 2010-08 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: The multipotency of human amniotic mesenchymal cells (HAMCs) has been reported, but the role of HAMCs in urinary tract regeneration is unknown. Objective: The aim of the study was to determine if cells derived from HAMCs support the structural and functional reconstruction of freeze-injured mouse bladders. Design, setting, and participants: HAMCs were harvested from an amnion membrane, and cells were cultured for 7 d prior to injection into the freeze-injured bladder walls of nude mice. Intervention: Three days prior to implantation, the posterior bladder walls were freeze injured for 30 s. The cultured HAMC-derived cells (0.5 x 10(5) cells per 50 mu l) were implanted into the injured regions. Control bladders received a cell-free injection. At 1, 2, 4, and 6 wk after the cell implantation, the experimental bladders were extirpated. Measurements: The bladder tissues were examined by immunohistochemistry for alpha-smooth muscle actin (SMA). The HAMC-derived cells were detected by antihuman nuclei antibody (HuNu). Separately, bladder muscle strips were examined for contractile responses to potassium. Results and limitations: At 1 wk after implantation, the HAMC-derived cells, which were detected by HuNu, differentiated into muscular layers composed of SMA-positive cells. From 2 to 6 wk after implantation, abundant layers of SMA-positive and HuNu-positive cells developed. In control bladders, few SMA-positive cells remained at the injured regions at 1 wk, but by 6 wk, more were present. At 1 wk, the contractile responses to potassium of the cell-implanted bladders were significantly higher than those of the control-injected ones. Control-injected bladders also recovered by 6 wk, but the rate of recovery was slower. Conclusions: Freeze-injured mouse bladders implanted with HAMC-derived cells recovered morphology and function faster than control-injected bladders. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0302-2838 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00639847 | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://pubmed.ncbi.nlm.nih.gov/20064685 | |||||
関連名称 | 20064685 | |||||
権利 | ||||||
権利情報 | Copyright© 2009 European Association of Urology. Published by Elsevier B. V. | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000279745100026 |