{"_buckets": {"deposit": "a7293c47-fff8-4b6f-9f41-c0930fee42ff"}, "_deposit": {"id": "8069", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "8069"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00008069"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "306", "bibliographicPageStart": "299", "bibliographicVolumeNumber": "58", "bibliographic_titles": [{"bibliographic_title": "EUROPEAN UROLOGY"}]}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Background: The multipotency of human amniotic mesenchymal cells (HAMCs) has been reported, but the role of HAMCs in urinary tract regeneration is unknown. Objective: The aim of the study was to determine if cells derived from HAMCs support the structural and functional reconstruction of freeze-injured mouse bladders. Design, setting, and participants: HAMCs were harvested from an amnion membrane, and cells were cultured for 7 d prior to injection into the freeze-injured bladder walls of nude mice. Intervention: Three days prior to implantation, the posterior bladder walls were freeze injured for 30 s. The cultured HAMC-derived cells (0.5 x 10(5) cells per 50 mu l) were implanted into the injured regions. Control bladders received a cell-free injection. At 1, 2, 4, and 6 wk after the cell implantation, the experimental bladders were extirpated. Measurements: The bladder tissues were examined by immunohistochemistry for alpha-smooth muscle actin (SMA). The HAMC-derived cells were detected by antihuman nuclei antibody (HuNu). Separately, bladder muscle strips were examined for contractile responses to potassium. Results and limitations: At 1 wk after implantation, the HAMC-derived cells, which were detected by HuNu, differentiated into muscular layers composed of SMA-positive cells. From 2 to 6 wk after implantation, abundant layers of SMA-positive and HuNu-positive cells developed. In control bladders, few SMA-positive cells remained at the injured regions at 1 wk, but by 6 wk, more were present. At 1 wk, the contractile responses to potassium of the cell-implanted bladders were significantly higher than those of the control-injected ones. Control-injected bladders also recovered by 6 wk, but the rate of recovery was slower. Conclusions: Freeze-injured mouse bladders implanted with HAMC-derived cells recovered morphology and function faster than control-injected bladders.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "EUROPEAN UROLOGY. 58(2):299-306 (2010)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Minagawa, Tomonori", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.jCfagUkh.html"}, {"subitem_link_text": "Imamura, Tetsuya", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.ypACOmyC.html"}, {"subitem_link_text": "Nishizawa, Osamu", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.WeyeWmyU.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000279745100026"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "ELSEVIER SCIENCE BV"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "20064685"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=20064685", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/j.eururo.2009.12.031"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.1016/j.eururo.2009.12.031", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "Copyright\u00a9 2009 European Association of Urology. Published by Elsevier B. V."}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0302-2838", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0302-2838", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00639847", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Regeneration; Cell transplantation; Mice; Urinary bladder; Bladder injury"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "STEM-CELLS; FLUID; REGENERATION; MEMBRANE; THERAPY"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Background: The multipotency of human amniotic mesenchymal cells (HAMCs) has been reported, but the role of HAMCs in urinary tract regeneration is unknown. Objective: The aim of the study was to determine if cells derived from HAMCs support the structural and functional reconstruction of freeze-injured mouse bladders. Design, setting, and participants: HAMCs were harvested from an amnion membrane, and cells were cultured for 7 d prior to injection into the freeze-injured bladder walls of nude mice. Intervention: Three days prior to implantation, the posterior bladder walls were freeze injured for 30 s. The cultured HAMC-derived cells (0.5 x 10(5) cells per 50 mu l) were implanted into the injured regions. Control bladders received a cell-free injection. At 1, 2, 4, and 6 wk after the cell implantation, the experimental bladders were extirpated. Measurements: The bladder tissues were examined by immunohistochemistry for alpha-smooth muscle actin (SMA). The HAMC-derived cells were detected by antihuman nuclei antibody (HuNu). Separately, bladder muscle strips were examined for contractile responses to potassium. Results and limitations: At 1 wk after implantation, the HAMC-derived cells, which were detected by HuNu, differentiated into muscular layers composed of SMA-positive cells. From 2 to 6 wk after implantation, abundant layers of SMA-positive and HuNu-positive cells developed. In control bladders, few SMA-positive cells remained at the injured regions at 1 wk, but by 6 wk, more were present. At 1 wk, the contractile responses to potassium of the cell-implanted bladders were significantly higher than those of the control-injected ones. Control-injected bladders also recovered by 6 wk, but the rate of recovery was slower. Conclusions: Freeze-injured mouse bladders implanted with HAMC-derived cells recovered morphology and function faster than control-injected bladders. (C) 2009 European Association of Urology. Published by Elsevier B. V. All rights reserved."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Minagawa,  Tomonori"}], "nameIdentifiers": [{"nameIdentifier": "22409", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imamura,  Tetsuya"}], "nameIdentifiers": [{"nameIdentifier": "22410", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Igawa,  Yasuhiko"}], "nameIdentifiers": [{"nameIdentifier": "22411", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Aizawa,  Naoki"}], "nameIdentifiers": [{"nameIdentifier": "22412", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishizuka,  Osamu"}], "nameIdentifiers": [{"nameIdentifier": "22413", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishizawa,  Osamu"}], "nameIdentifiers": [{"nameIdentifier": "22414", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Differentiation_Smooth_Muscle_Cells_Human_Amniotic_Mesenchymal_Cells.pdf", "filesize": [{"value": "1.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1800000.0, "url": {"label": "Differentiation_Smooth_Muscle_Cells_Human_Amniotic_Mesenchymal_Cells.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/8069/files/Differentiation_Smooth_Muscle_Cells_Human_Amniotic_Mesenchymal_Cells.pdf"}, "version_id": "114dd067-5053-4358-a568-c2d4c7c87c4d"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Regeneration", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cell transplantation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Mice", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Urinary bladder", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Bladder injury", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Differentiation of Smooth Muscle Cells from Human Amniotic Mesenchymal Cells Implanted in the Freeze-Injured Mouse Urinary Bladder", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Differentiation of Smooth Muscle Cells from Human Amniotic Mesenchymal Cells Implanted in the Freeze-Injured Mouse Urinary Bladder"}]}, "item_type_id": "6", "owner": "1", "path": ["882/883"], "permalink_uri": "http://hdl.handle.net/10091/16879", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2013-04-01"}, "publish_date": "2013-04-01", "publish_status": "0", "recid": "8069", "relation": {}, "relation_version_is_last": true, "title": ["Differentiation of Smooth Muscle Cells from Human Amniotic Mesenchymal Cells Implanted in the Freeze-Injured Mouse Urinary Bladder"], "weko_shared_id": null}