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Methods: We developed a strain of gmLAB, Lactococcus lactis NZ9000 (NZ-HO), which secretes the anti-inflammatory molecule recombinant mouse heme oxygenase-1 (rmHO-1). The effects of short-term continuous oral dosing with NZ-HO were evaluated in mice with dextran sulfate sodium (DSS)-induced acute colitis as a model of inflammatory bowel diseases (IBD). Results: We identified the secretion of rmHO-1 by NZ-HO. rmHO-1 was biologically active as determined with spectroscopy. Viable NZ-HO was directly delivered to the colon via oral administration, and rmHO-1 was secreted onto the colonic mucosa in mice. Acute colitis in mice was induced by free drinking of 3 % DSS in water and was accompanied by an increase in the disease activity index score and histopathological changes. Daily oral administration of NZ-HO significantly improved these colitis-associated symptoms. In addition, NZ-HO significantly increased production of the anti-inflammatory cytokine interleukin (IL)-10 and decreased the expression of pro-inflammatory cytokines such as IL-1 alpha and IL-6 in the colon compared to a vector control strain. Conclusions: Oral administration of NZ-HO alleviates DSS-induced acute colitis in mice. 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Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice
http://hdl.handle.net/10091/00018785
http://hdl.handle.net/10091/00018785ad35fc8b-b3ab-41c7-843a-93a49a3e2e2e
名前 / ファイル | ライセンス | アクション |
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Oral_delivery_Lactococcus_lactis_secretes.pdf (6.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-04-22 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice | |||||
言語 | ||||||
言語 | eng | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1186/s12934-015-0378-2 | |||||
関連名称 | 10.1186/s12934-015-0378-2 | |||||
キーワード | ||||||
主題 | Lactococcus lactis, Heme oxygenase-1, Acute colitis, Interleukin-10, Mice, Inflammatory bowel disease | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Shigemori, Suguru
× Shigemori, Suguru× Watanabe, Takafumi× Kudoh, Kai× Ihara, Masaki× Nigar, Shireen× Yamamoto, Yoshinari× Suda, Yoshihito× Sato, Takashi× Kitazawa, Haruki× Shimosato, Takeshi |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Ihara, Masaki | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jpkaWUyV.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Shimosato, Takeshi | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html | |||||
出版者 | ||||||
出版者 | BIOMED CENTRAL LTD | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | MICROBIAL CELL FACTORIES. 14:189 (2015) | |||||
書誌情報 |
MICROBIAL CELL FACTORIES 巻 14, 発行日 2015-11-15 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Mucosal delivery of therapeutic proteins using genetically modified strains of lactic acid bacteria (gmLAB) is being investigated as a new therapeutic strategy. Methods: We developed a strain of gmLAB, Lactococcus lactis NZ9000 (NZ-HO), which secretes the anti-inflammatory molecule recombinant mouse heme oxygenase-1 (rmHO-1). The effects of short-term continuous oral dosing with NZ-HO were evaluated in mice with dextran sulfate sodium (DSS)-induced acute colitis as a model of inflammatory bowel diseases (IBD). Results: We identified the secretion of rmHO-1 by NZ-HO. rmHO-1 was biologically active as determined with spectroscopy. Viable NZ-HO was directly delivered to the colon via oral administration, and rmHO-1 was secreted onto the colonic mucosa in mice. Acute colitis in mice was induced by free drinking of 3 % DSS in water and was accompanied by an increase in the disease activity index score and histopathological changes. Daily oral administration of NZ-HO significantly improved these colitis-associated symptoms. In addition, NZ-HO significantly increased production of the anti-inflammatory cytokine interleukin (IL)-10 and decreased the expression of pro-inflammatory cytokines such as IL-1 alpha and IL-6 in the colon compared to a vector control strain. Conclusions: Oral administration of NZ-HO alleviates DSS-induced acute colitis in mice. Our results suggest that NZ-HO may be a useful mucosal therapeutic agent for treating IBD. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1475-2859 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12039472 | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://pubmed.ncbi.nlm.nih.gov/26608030/ | |||||
関連名称 | 26608030 | |||||
権利 | ||||||
権利情報 | Copyright © Shigemori et al. 2015 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000365270300002 |