{"_buckets": {"deposit": "fa29a700-f826-4dbb-b939-54c158d35521"}, "_deposit": {"id": "18017", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "18017"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00018017"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-11-15", "bibliographicIssueDateType": "Issued"}, "bibliographicVolumeNumber": "14", "bibliographic_titles": [{"bibliographic_title": "MICROBIAL CELL FACTORIES"}]}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Background: Mucosal delivery of therapeutic proteins using genetically modified strains of lactic acid bacteria (gmLAB) is being investigated as a new therapeutic strategy. Methods: We developed a strain of gmLAB, Lactococcus lactis NZ9000 (NZ-HO), which secretes the anti-inflammatory molecule recombinant mouse heme oxygenase-1 (rmHO-1). The effects of short-term continuous oral dosing with NZ-HO were evaluated in mice with dextran sulfate sodium (DSS)-induced acute colitis as a model of inflammatory bowel diseases (IBD). Results: We identified the secretion of rmHO-1 by NZ-HO. rmHO-1 was biologically active as determined with spectroscopy. Viable NZ-HO was directly delivered to the colon via oral administration, and rmHO-1 was secreted onto the colonic mucosa in mice. Acute colitis in mice was induced by free drinking of 3 % DSS in water and was accompanied by an increase in the disease activity index score and histopathological changes. Daily oral administration of NZ-HO significantly improved these colitis-associated symptoms. In addition, NZ-HO significantly increased production of the anti-inflammatory cytokine interleukin (IL)-10 and decreased the expression of pro-inflammatory cytokines such as IL-1 alpha and IL-6 in the colon compared to a vector control strain. Conclusions: Oral administration of NZ-HO alleviates DSS-induced acute colitis in mice. Our results suggest that NZ-HO may be a useful mucosal therapeutic agent for treating IBD.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "MICROBIAL CELL FACTORIES. 14:189 (2015)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Ihara, Masaki", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.jpkaWUyV.html"}, {"subitem_link_text": "Shimosato, Takeshi", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000365270300002"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "BIOMED CENTRAL LTD"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "26608030"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=26608030", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1186/s12934-015-0378-2"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.1186/s12934-015-0378-2", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "Copyright \u00a9 Shigemori et al. 2015"}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1475-2859", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1475-2859", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12039472", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Lactococcus lactis; Heme oxygenase-1; Acute colitis; Interleukin-10; Mice; Inflammatory bowel disease"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "INFLAMMATORY-BOWEL-DISEASE; CARBON-MONOXIDE; MURINE COLITIS; INTESTINAL INFLAMMATION; ACID BACTERIA; INTERLEUKIN-10 RECEPTOR; THERAPEUTIC TARGET; CHRONIC SILICOSIS; DEGRADATION; EXPRESSION"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Background: Mucosal delivery of therapeutic proteins using genetically modified strains of lactic acid bacteria (gmLAB) is being investigated as a new therapeutic strategy. Methods: We developed a strain of gmLAB, Lactococcus lactis NZ9000 (NZ-HO), which secretes the anti-inflammatory molecule recombinant mouse heme oxygenase-1 (rmHO-1). The effects of short-term continuous oral dosing with NZ-HO were evaluated in mice with dextran sulfate sodium (DSS)-induced acute colitis as a model of inflammatory bowel diseases (IBD). Results: We identified the secretion of rmHO-1 by NZ-HO. rmHO-1 was biologically active as determined with spectroscopy. Viable NZ-HO was directly delivered to the colon via oral administration, and rmHO-1 was secreted onto the colonic mucosa in mice. Acute colitis in mice was induced by free drinking of 3 % DSS in water and was accompanied by an increase in the disease activity index score and histopathological changes. Daily oral administration of NZ-HO significantly improved these colitis-associated symptoms. In addition, NZ-HO significantly increased production of the anti-inflammatory cytokine interleukin (IL)-10 and decreased the expression of pro-inflammatory cytokines such as IL-1 alpha and IL-6 in the colon compared to a vector control strain. Conclusions: Oral administration of NZ-HO alleviates DSS-induced acute colitis in mice. Our results suggest that NZ-HO may be a useful mucosal therapeutic agent for treating IBD."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Shigemori,  Suguru"}], "nameIdentifiers": [{"nameIdentifier": "50381", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe,  Takafumi"}], "nameIdentifiers": [{"nameIdentifier": "50382", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kudoh,  Kai"}], "nameIdentifiers": [{"nameIdentifier": "50383", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ihara,  Masaki"}], "nameIdentifiers": [{"nameIdentifier": "50384", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nigar,  Shireen"}], "nameIdentifiers": [{"nameIdentifier": "50385", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamamoto,  Yoshinari"}], "nameIdentifiers": [{"nameIdentifier": "50386", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suda,  Yoshihito"}], "nameIdentifiers": [{"nameIdentifier": "50387", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato,  Takashi"}], "nameIdentifiers": [{"nameIdentifier": "50388", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitazawa,  Haruki"}], "nameIdentifiers": [{"nameIdentifier": "50389", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimosato,  Takeshi"}], "nameIdentifiers": [{"nameIdentifier": "50390", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-04-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Oral_delivery_Lactococcus_lactis_secretes.pdf", "filesize": [{"value": "6.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 6000000.0, "url": {"label": "Oral_delivery_Lactococcus_lactis_secretes.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/18017/files/Oral_delivery_Lactococcus_lactis_secretes.pdf"}, "version_id": "6d46e95b-7d8d-4614-ab62-435afb139266"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Lactococcus lactis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Heme oxygenase-1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Acute colitis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Interleukin-10", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Mice", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Inflammatory bowel disease", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice"}]}, "item_type_id": "6", "owner": "1", "path": ["1016/1017"], "permalink_uri": "http://hdl.handle.net/10091/00018785", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2016-04-22"}, "publish_date": "2016-04-22", "publish_status": "0", "recid": "18017", "relation": {}, "relation_version_is_last": true, "title": ["Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice"], "weko_shared_id": 3}